Capsular typing of Streptococcus pneumoniae isolated in an Algerian hospital using a new multiplex PCR-based scheme
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.18/3730 |
Resumo: | We developed a new sequential multiplex-PCR-based typing scheme (MPBTS) for pneumococcal capsular classification. The serogroup/type of 37 control isolates obtained by the Quellung reaction, MPBTS, and nucleotide sequencing, were fully concordant. The serogroups/types of 75 invasive isolates determined by MPBTS, presented 100% specificity and 96% sensitivity, when compared with the Quellung reaction. |
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Capsular typing of Streptococcus pneumoniae isolated in an Algerian hospital using a new multiplex PCR-based schemeMultiplex PCRPneumococciQuellung ReactionSensitivitySpecificitySerogroup/typeResistência aos AntimicrobianosWe developed a new sequential multiplex-PCR-based typing scheme (MPBTS) for pneumococcal capsular classification. The serogroup/type of 37 control isolates obtained by the Quellung reaction, MPBTS, and nucleotide sequencing, were fully concordant. The serogroups/types of 75 invasive isolates determined by MPBTS, presented 100% specificity and 96% sensitivity, when compared with the Quellung reaction.H. Ziane was supported by a grant from Ministère de l'Enseignement Superieur et de la Recherche Scientifique in Algeria. V. Manageiro was supported by the grant SFRH/BPD/77486/2011, from Fundação para a Ciência e a Tecnologia, Lisbon, Portugal.ElsevierRepositório Científico do Instituto Nacional de SaúdeZiane, HanifaManageiro, VeraFerreira, EugéniaBektache, SoumiaTazir, MohamedCaniça, Manuela2020-12-01T01:30:10Z20152015-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.18/3730engJ Microbiol Methods. 2015 Dec;119:243-6. doi: 10.1016/j.mimet.2015.11.002. Epub 2015 Nov 4.0167-701210.1016/j.mimet.2015.11.002info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-20T15:39:57Zoai:repositorio.insa.pt:10400.18/3730Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:38:34.339080Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Capsular typing of Streptococcus pneumoniae isolated in an Algerian hospital using a new multiplex PCR-based scheme |
title |
Capsular typing of Streptococcus pneumoniae isolated in an Algerian hospital using a new multiplex PCR-based scheme |
spellingShingle |
Capsular typing of Streptococcus pneumoniae isolated in an Algerian hospital using a new multiplex PCR-based scheme Ziane, Hanifa Multiplex PCR Pneumococci Quellung Reaction Sensitivity Specificity Serogroup/type Resistência aos Antimicrobianos |
title_short |
Capsular typing of Streptococcus pneumoniae isolated in an Algerian hospital using a new multiplex PCR-based scheme |
title_full |
Capsular typing of Streptococcus pneumoniae isolated in an Algerian hospital using a new multiplex PCR-based scheme |
title_fullStr |
Capsular typing of Streptococcus pneumoniae isolated in an Algerian hospital using a new multiplex PCR-based scheme |
title_full_unstemmed |
Capsular typing of Streptococcus pneumoniae isolated in an Algerian hospital using a new multiplex PCR-based scheme |
title_sort |
Capsular typing of Streptococcus pneumoniae isolated in an Algerian hospital using a new multiplex PCR-based scheme |
author |
Ziane, Hanifa |
author_facet |
Ziane, Hanifa Manageiro, Vera Ferreira, Eugénia Bektache, Soumia Tazir, Mohamed Caniça, Manuela |
author_role |
author |
author2 |
Manageiro, Vera Ferreira, Eugénia Bektache, Soumia Tazir, Mohamed Caniça, Manuela |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Repositório Científico do Instituto Nacional de Saúde |
dc.contributor.author.fl_str_mv |
Ziane, Hanifa Manageiro, Vera Ferreira, Eugénia Bektache, Soumia Tazir, Mohamed Caniça, Manuela |
dc.subject.por.fl_str_mv |
Multiplex PCR Pneumococci Quellung Reaction Sensitivity Specificity Serogroup/type Resistência aos Antimicrobianos |
topic |
Multiplex PCR Pneumococci Quellung Reaction Sensitivity Specificity Serogroup/type Resistência aos Antimicrobianos |
description |
We developed a new sequential multiplex-PCR-based typing scheme (MPBTS) for pneumococcal capsular classification. The serogroup/type of 37 control isolates obtained by the Quellung reaction, MPBTS, and nucleotide sequencing, were fully concordant. The serogroups/types of 75 invasive isolates determined by MPBTS, presented 100% specificity and 96% sensitivity, when compared with the Quellung reaction. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015 2015-01-01T00:00:00Z 2020-12-01T01:30:10Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.18/3730 |
url |
http://hdl.handle.net/10400.18/3730 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
J Microbiol Methods. 2015 Dec;119:243-6. doi: 10.1016/j.mimet.2015.11.002. Epub 2015 Nov 4. 0167-7012 10.1016/j.mimet.2015.11.002 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799132122713161728 |