Age-associated distribution of normal B-cell and plasma cell subsets in peripheral blood
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2018 |
| Outros Autores: | , , , , , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/10451/53064 |
Resumo: | © 2018 The Authors. Published by Elsevier Inc. on behalf of the American Academy of Allergy, Asthma & Immunology. This is an open access article under the CC BY-NC- ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
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Age-associated distribution of normal B-cell and plasma cell subsets in peripheral bloodIgH isotypeImmunoglobulinsAge-related valuesFlow cytometryMemory B cellsNormal B cellsPlasma cellsReference rangesSubclass© 2018 The Authors. Published by Elsevier Inc. on behalf of the American Academy of Allergy, Asthma & Immunology. This is an open access article under the CC BY-NC- ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).Background: Humoral immunocompetence develops stepwise throughout life and contributes to individual susceptibility to infection, immunodeficiency, autoimmunity, and neoplasia. Immunoglobulin heavy chain (IgH) isotype serum levels can partly explain such age-related differences, but their relationship with the IgH isotype distribution within memory B-cell (MBC) and plasma cell (PCs) compartments remains to be investigated. Objective: We studied the age-related distribution of MBCs and PCs expressing different IgH isotypes in addition to the immature/transitional and naive B-cell compartments. Methods: B-cell and PC subsets and plasma IgH isotype levels were studied in cord blood (n = 19) and peripheral blood (n = 215) from healthy donors aged 0 to 90 years by using flow cytometry and nephelometry, respectively. Results: IgH-switched MBCs expressing IgG1, IgG2, IgG3, IgA1, and IgA2 were already detected in cord blood and newborns at very low counts, whereas CD27+IgM++IgD+ MBCs only became detectable at 1 to 5 months and remained stable until 2 to 4 years, and IgD MBCs peaked at 2 to 4 years, with both populations decreasing thereafter. MBCs expressing IgH isotypes of the second immunoglobulin heavy chain constant region (IGHC) gene block (IgG1, IgG3, and IgA1) peaked later during childhood (2-4 years), whereas MBCs expressing third IGHC gene block immunoglobulin isotypes (IgG2, IgG4, and IgA2) reached maximum values during adulthood. PCs were already detected in newborns, increasing in number until 6 to 11 months for IgM, IgG1, IgG2, IgG3, IgA1, and IgA2; until 2 to 4 years for IgD; and until 5 to 9 years for IgG4 and decreasing thereafter. For most IgH isotypes (except IgD and IgG4), maximum plasma levels were reached after PC and MBC counts peaked. Conclusions: PC counts reach maximum values early in life, followed by MBC counts and plasma IgH isotypes. Importantly, IgH isotypes from different IGHC gene blocks show different patterns, probably reflecting consecutive cycles of IgH isotype switch recombination through life.E.B. was supported by a grant from Junta de Castilla y León (Fondo Social Europeo, ORDEN EDU/346/2013, Valladolid, Spain). This work was supported by the CB16/12/00400 grant (CIBERONC, Instituto de Salud Carlos III, Ministerio de Economía y Competitividad, Madrid, Spain, and FONDOS FEDER) and the FIS PI12/00905-FEDER grant from the Fondo de Investigaciones Sanitarias of Instituto de Salud Carlos III (Madrid, Spain). O.P. and T.K. were supported by the Ministry of Education, Youth and Sports (NPU I no. LO1604 and 15-28541A). The coordination and innovation processes of this study were supported by the EuroFlow Consortium.ElsevierRepositório da Universidade de LisboaBlanco, ElenaPérez-Andrés, MartínArriba-Méndez, SoniaContreras-Sanfeliciano, TeresaCriado, IgnacioPelak, OndrejSerra-Caetano, AnaRomero, AlfonsoPuig, NoemíRemesal, AnaTorres Canizales, JuanLópez-Granados, EduardoKalina, TomasSousa, Ana E.van Zelm, Mennovan der Burg, Mirjamvan Dongen, Jacques J. M.Orfao, Alberto2022-05-19T13:27:52Z20182018-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/53064engJ Allergy Clin Immunol. 2018 Jun;141(6):2208-2219.e160091-674910.1016/j.jaci.2018.02.0171097-6825info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T16:58:37Zoai:repositorio.ul.pt:10451/53064Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T22:04:01.288007Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
Age-associated distribution of normal B-cell and plasma cell subsets in peripheral blood |
| title |
Age-associated distribution of normal B-cell and plasma cell subsets in peripheral blood |
| spellingShingle |
Age-associated distribution of normal B-cell and plasma cell subsets in peripheral blood Blanco, Elena IgH isotype Immunoglobulins Age-related values Flow cytometry Memory B cells Normal B cells Plasma cells Reference ranges Subclass |
| title_short |
Age-associated distribution of normal B-cell and plasma cell subsets in peripheral blood |
| title_full |
Age-associated distribution of normal B-cell and plasma cell subsets in peripheral blood |
| title_fullStr |
Age-associated distribution of normal B-cell and plasma cell subsets in peripheral blood |
| title_full_unstemmed |
Age-associated distribution of normal B-cell and plasma cell subsets in peripheral blood |
| title_sort |
Age-associated distribution of normal B-cell and plasma cell subsets in peripheral blood |
| author |
Blanco, Elena |
| author_facet |
Blanco, Elena Pérez-Andrés, Martín Arriba-Méndez, Sonia Contreras-Sanfeliciano, Teresa Criado, Ignacio Pelak, Ondrej Serra-Caetano, Ana Romero, Alfonso Puig, Noemí Remesal, Ana Torres Canizales, Juan López-Granados, Eduardo Kalina, Tomas Sousa, Ana E. van Zelm, Menno van der Burg, Mirjam van Dongen, Jacques J. M. Orfao, Alberto |
| author_role |
author |
| author2 |
Pérez-Andrés, Martín Arriba-Méndez, Sonia Contreras-Sanfeliciano, Teresa Criado, Ignacio Pelak, Ondrej Serra-Caetano, Ana Romero, Alfonso Puig, Noemí Remesal, Ana Torres Canizales, Juan López-Granados, Eduardo Kalina, Tomas Sousa, Ana E. van Zelm, Menno van der Burg, Mirjam van Dongen, Jacques J. M. Orfao, Alberto |
| author2_role |
author author author author author author author author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Repositório da Universidade de Lisboa |
| dc.contributor.author.fl_str_mv |
Blanco, Elena Pérez-Andrés, Martín Arriba-Méndez, Sonia Contreras-Sanfeliciano, Teresa Criado, Ignacio Pelak, Ondrej Serra-Caetano, Ana Romero, Alfonso Puig, Noemí Remesal, Ana Torres Canizales, Juan López-Granados, Eduardo Kalina, Tomas Sousa, Ana E. van Zelm, Menno van der Burg, Mirjam van Dongen, Jacques J. M. Orfao, Alberto |
| dc.subject.por.fl_str_mv |
IgH isotype Immunoglobulins Age-related values Flow cytometry Memory B cells Normal B cells Plasma cells Reference ranges Subclass |
| topic |
IgH isotype Immunoglobulins Age-related values Flow cytometry Memory B cells Normal B cells Plasma cells Reference ranges Subclass |
| description |
© 2018 The Authors. Published by Elsevier Inc. on behalf of the American Academy of Allergy, Asthma & Immunology. This is an open access article under the CC BY-NC- ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
| publishDate |
2018 |
| dc.date.none.fl_str_mv |
2018 2018-01-01T00:00:00Z 2022-05-19T13:27:52Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10451/53064 |
| url |
http://hdl.handle.net/10451/53064 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
J Allergy Clin Immunol. 2018 Jun;141(6):2208-2219.e16 0091-6749 10.1016/j.jaci.2018.02.017 1097-6825 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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Elsevier |
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Elsevier |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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