Exploiting intrinsinc fluorescence spectroscopy to discriminate Acinetobacter calcoaceticus- Acinetobacter baumannii complex species
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/44513 |
Resumo: | Several spectroscopic techniques, as infrared, Raman, fluorescence and/or mass spectrometry, have been tested in the context of bacterial typing being the degree of success highly dependent of the taxonomic level. Instrinsic fluorescence spectroscopy, due to the natural bacterial fluorophores, have been claimed to be a reliable alternative to the standard typing methods with some published works reporting its success. In this work we evaluate the ability of this technique to discriminate four closely related species belonging to the so-called Acinetobacter calcoaceticus Acinetobacter baumannii complex. Single-point and two dimensional fluorescence spectral data were acquired at room temperature and 25ºC. Spectra were analysed by partial least squares discriminant analysis and soft independent modelling of class analogy. The percentage of correct species assignments, ranging from 4.2 - 97.7%, is highly dependent of the experimental conditions and the data analysis. It seems that the results benefit from a strictly temperature control being those achieved with two-dimensional data slightly better. Nevertheless, it was impossible to achieve a satisfactory percentage of correct assignments for the four species simultaneously pointing to several limitations of this technique for such purposes. |
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Exploiting intrinsinc fluorescence spectroscopy to discriminate Acinetobacter calcoaceticus- Acinetobacter baumannii complex speciesScience & TechnologySeveral spectroscopic techniques, as infrared, Raman, fluorescence and/or mass spectrometry, have been tested in the context of bacterial typing being the degree of success highly dependent of the taxonomic level. Instrinsic fluorescence spectroscopy, due to the natural bacterial fluorophores, have been claimed to be a reliable alternative to the standard typing methods with some published works reporting its success. In this work we evaluate the ability of this technique to discriminate four closely related species belonging to the so-called Acinetobacter calcoaceticus Acinetobacter baumannii complex. Single-point and two dimensional fluorescence spectral data were acquired at room temperature and 25ºC. Spectra were analysed by partial least squares discriminant analysis and soft independent modelling of class analogy. The percentage of correct species assignments, ranging from 4.2 - 97.7%, is highly dependent of the experimental conditions and the data analysis. It seems that the results benefit from a strictly temperature control being those achieved with two-dimensional data slightly better. Nevertheless, it was impossible to achieve a satisfactory percentage of correct assignments for the four species simultaneously pointing to several limitations of this technique for such purposes.This work received financial support from the European Union (FEDER funds POCI/01/0145/FEDER/007265) and National Funds (FCT/MEC, Fundação para a Ciência e Tecnologia and Ministério da Educação e Ciência) under the Partnership Agreement PT2020 UID/QUI/50006/2013. Cristina Quintelas was supported by a postdoctoral grant (SFRH/BPD/101338/2014). Clara Sousa was funded through the NORTE-01-0145-FEDER-000024 – “New Technologies for three Health Challenges of Modern Societies: Diabetes, Drug Abuse and Kidney Diseases”. Thanks are due to Alexandr Nemec from the Laboratory of Bacterial Genetics, National Institute of Public Health, Prague – Czech Republic, for providing all the bacterial isolates used in this work.Royal Society of ChemistryUniversidade do MinhoSilva, Ana SofiaQuintelas, CristinaFerreira, Eugénio C.Nemec, AlexandrLopes, João A.Sousa, C.2017-01-142017-01-14T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/44513engSilva, Ana Sofia; Quintelas, Cristina; Ferreira, Eugénio C.; Nemec, Alexandr; Lopes, João A.; Sousa, C., Exploiting intrinsinc fluorescence spectroscopy to discriminate Acinetobacter calcoaceticus- Acinetobacter baumannii complex species. RSC Advances, 7, 8581-8588, 20172046-20692046-206910.1039/c6ra27531ahttp://pubs.rsc.org/en/journals/journalissues/rainfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:45:43Zoai:repositorium.sdum.uminho.pt:1822/44513Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:43:36.632222Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Exploiting intrinsinc fluorescence spectroscopy to discriminate Acinetobacter calcoaceticus- Acinetobacter baumannii complex species |
title |
Exploiting intrinsinc fluorescence spectroscopy to discriminate Acinetobacter calcoaceticus- Acinetobacter baumannii complex species |
spellingShingle |
Exploiting intrinsinc fluorescence spectroscopy to discriminate Acinetobacter calcoaceticus- Acinetobacter baumannii complex species Silva, Ana Sofia Science & Technology |
title_short |
Exploiting intrinsinc fluorescence spectroscopy to discriminate Acinetobacter calcoaceticus- Acinetobacter baumannii complex species |
title_full |
Exploiting intrinsinc fluorescence spectroscopy to discriminate Acinetobacter calcoaceticus- Acinetobacter baumannii complex species |
title_fullStr |
Exploiting intrinsinc fluorescence spectroscopy to discriminate Acinetobacter calcoaceticus- Acinetobacter baumannii complex species |
title_full_unstemmed |
Exploiting intrinsinc fluorescence spectroscopy to discriminate Acinetobacter calcoaceticus- Acinetobacter baumannii complex species |
title_sort |
Exploiting intrinsinc fluorescence spectroscopy to discriminate Acinetobacter calcoaceticus- Acinetobacter baumannii complex species |
author |
Silva, Ana Sofia |
author_facet |
Silva, Ana Sofia Quintelas, Cristina Ferreira, Eugénio C. Nemec, Alexandr Lopes, João A. Sousa, C. |
author_role |
author |
author2 |
Quintelas, Cristina Ferreira, Eugénio C. Nemec, Alexandr Lopes, João A. Sousa, C. |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Silva, Ana Sofia Quintelas, Cristina Ferreira, Eugénio C. Nemec, Alexandr Lopes, João A. Sousa, C. |
dc.subject.por.fl_str_mv |
Science & Technology |
topic |
Science & Technology |
description |
Several spectroscopic techniques, as infrared, Raman, fluorescence and/or mass spectrometry, have been tested in the context of bacterial typing being the degree of success highly dependent of the taxonomic level. Instrinsic fluorescence spectroscopy, due to the natural bacterial fluorophores, have been claimed to be a reliable alternative to the standard typing methods with some published works reporting its success. In this work we evaluate the ability of this technique to discriminate four closely related species belonging to the so-called Acinetobacter calcoaceticus Acinetobacter baumannii complex. Single-point and two dimensional fluorescence spectral data were acquired at room temperature and 25ºC. Spectra were analysed by partial least squares discriminant analysis and soft independent modelling of class analogy. The percentage of correct species assignments, ranging from 4.2 - 97.7%, is highly dependent of the experimental conditions and the data analysis. It seems that the results benefit from a strictly temperature control being those achieved with two-dimensional data slightly better. Nevertheless, it was impossible to achieve a satisfactory percentage of correct assignments for the four species simultaneously pointing to several limitations of this technique for such purposes. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-01-14 2017-01-14T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/44513 |
url |
http://hdl.handle.net/1822/44513 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Silva, Ana Sofia; Quintelas, Cristina; Ferreira, Eugénio C.; Nemec, Alexandr; Lopes, João A.; Sousa, C., Exploiting intrinsinc fluorescence spectroscopy to discriminate Acinetobacter calcoaceticus- Acinetobacter baumannii complex species. RSC Advances, 7, 8581-8588, 2017 2046-2069 2046-2069 10.1039/c6ra27531a http://pubs.rsc.org/en/journals/journalissues/ra |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Royal Society of Chemistry |
publisher.none.fl_str_mv |
Royal Society of Chemistry |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799132993257734144 |