Copper-containing nitrite reductase from Pseudomonas chlororaphis DSM 50135

Detalhes bibliográficos
Autor(a) principal: Moura, Isabel
Data de Publicação: 2004
Outros Autores: Pinho, Dora, Besson, Stéphane, Brondino, Carlos D., Castro, Baltazar de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/1652
Resumo: Eur. J. Biochem. 271, 2361–2369 (2004)
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spelling Copper-containing nitrite reductase from Pseudomonas chlororaphis DSM 50135Evidence for modulation of the rate of intramolecular electron transfer through nitrite binding to the type 2 copper centerCopper nitrite reductaseEPRRedox-titrationType 1 copperType 2 copperEur. J. Biochem. 271, 2361–2369 (2004)The nitrite reductase (Nir) isolated from Pseudomonas chlororaphis DSM 50135 is a blue enzyme, with type 1 and type 2 copper centers, as in all copper-containing Nirs described so far. For the first time, a direct determination of the reduction potentials of both copper centers in a Cu-Nir was performed: type 2 copper (T2Cu), 172 mV and type 1 copper (T1Cu), 298 mV at pH 7.6. Although the obtained values seem to be inconsistent with the established electrontransfer mechanism, EPR data indicate that the binding of nitrite to the T2Cu center increases its potential, favoring the electron-transfer process. Analysis of the EPR spectrum of the turnover form of the enzyme also suggests that the electron-transfer process between T1Cu and T2Cu is the fastest of the three redox processes involved in the catalysis: (a) reduction of T1Cu; (b) oxidation of T1Cu by T2Cu; and(c) reoxidation of T2Cu by NO2 –. Electrochemical experiments showthat azurin from the same organism can donate electrons to this enzyme.Blackwell PublishingRUNMoura, IsabelPinho, DoraBesson, StéphaneBrondino, Carlos D.Castro, Baltazar de2008-09-22T14:45:32Z20042004-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/1652eng1432-1033info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T03:31:37Zoai:run.unl.pt:10362/1652Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:14:46.708991Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Copper-containing nitrite reductase from Pseudomonas chlororaphis DSM 50135
Evidence for modulation of the rate of intramolecular electron transfer through nitrite binding to the type 2 copper center
title Copper-containing nitrite reductase from Pseudomonas chlororaphis DSM 50135
spellingShingle Copper-containing nitrite reductase from Pseudomonas chlororaphis DSM 50135
Moura, Isabel
Copper nitrite reductase
EPR
Redox-titration
Type 1 copper
Type 2 copper
title_short Copper-containing nitrite reductase from Pseudomonas chlororaphis DSM 50135
title_full Copper-containing nitrite reductase from Pseudomonas chlororaphis DSM 50135
title_fullStr Copper-containing nitrite reductase from Pseudomonas chlororaphis DSM 50135
title_full_unstemmed Copper-containing nitrite reductase from Pseudomonas chlororaphis DSM 50135
title_sort Copper-containing nitrite reductase from Pseudomonas chlororaphis DSM 50135
author Moura, Isabel
author_facet Moura, Isabel
Pinho, Dora
Besson, Stéphane
Brondino, Carlos D.
Castro, Baltazar de
author_role author
author2 Pinho, Dora
Besson, Stéphane
Brondino, Carlos D.
Castro, Baltazar de
author2_role author
author
author
author
dc.contributor.none.fl_str_mv RUN
dc.contributor.author.fl_str_mv Moura, Isabel
Pinho, Dora
Besson, Stéphane
Brondino, Carlos D.
Castro, Baltazar de
dc.subject.por.fl_str_mv Copper nitrite reductase
EPR
Redox-titration
Type 1 copper
Type 2 copper
topic Copper nitrite reductase
EPR
Redox-titration
Type 1 copper
Type 2 copper
description Eur. J. Biochem. 271, 2361–2369 (2004)
publishDate 2004
dc.date.none.fl_str_mv 2004
2004-01-01T00:00:00Z
2008-09-22T14:45:32Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/1652
url http://hdl.handle.net/10362/1652
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1432-1033
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Blackwell Publishing
publisher.none.fl_str_mv Blackwell Publishing
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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