Biochemical Characterization of the Copper Nitrite Reductase from Neisseria gonorrhoeae

Detalhes bibliográficos
Autor(a) principal: Barreiro, Daniela S.
Data de Publicação: 2023
Outros Autores: Oliveira, Ricardo N. S., Pauleta, Sofia R.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/157966
Resumo: Publisher Copyright: © 2023 by the authors.
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spelling Biochemical Characterization of the Copper Nitrite Reductase from Neisseria gonorrhoeaeAniAcopper enzymecopper nitrite reductasehyperthermostableNeisserianitrite reductionspectroscopyT1 copper centerT2 copper centerthermostabilityBiochemistryMolecular BiologyBiophysicsSDG 3 - Good Health and Well-beingPublisher Copyright: © 2023 by the authors.The copper-containing nitrite reductase from Neisseria gonorrhoeae has been shown to play a critical role in the infection mechanism of this microorganism by producing NO and abolishing epithelial exfoliation. This enzyme is a trimer with a type 1 copper center per subunit and a type 2 copper center in the subunits interface, with the latter being the catalytic site. The two centers were characterized for the first time by EPR and CD spectroscopy, showing that the type 1 copper center has a high rhombicity due to its lower symmetry and more tetragonal structure, while the type 2 copper center has the usual properties, but with a smaller hyperfine coupling constant (A// = 10.5 mT). The thermostability of the enzyme was analyzed by differential scanning calorimetry, which shows a single endothermic transition in the thermogram, with a maximum at 94 °C, while the CD spectra in the visible region indicate the presence of the type 1 copper center up to 80 °C. The reoxidation of the N. gonorrhoeae copper-containing nitrite reductase in the presence of nitrite were analyzed by visible spectroscopy and showed a pH dependence, being higher at pH 5.5–6.0. The high thermostability of this enzyme may be important to maintaining a high activity in the extracellular space and to making it less susceptible to denaturation and proteolysis, contributing to the proliferation of N. gonorrhoeae.DQ - Departamento de QuímicaUCIBIO - Applied Molecular Biosciences UnitRUNBarreiro, Daniela S.Oliveira, Ricardo N. S.Pauleta, Sofia R.2023-09-18T22:13:43Z2023-08-042023-08-04T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article15application/pdfhttp://hdl.handle.net/10362/157966eng2218-273XPURE: 71829200https://doi.org/10.3390/biom13081215info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:40:14Zoai:run.unl.pt:10362/157966Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:56:55.912372Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Biochemical Characterization of the Copper Nitrite Reductase from Neisseria gonorrhoeae
title Biochemical Characterization of the Copper Nitrite Reductase from Neisseria gonorrhoeae
spellingShingle Biochemical Characterization of the Copper Nitrite Reductase from Neisseria gonorrhoeae
Barreiro, Daniela S.
AniA
copper enzyme
copper nitrite reductase
hyperthermostable
Neisseria
nitrite reduction
spectroscopy
T1 copper center
T2 copper center
thermostability
Biochemistry
Molecular Biology
Biophysics
SDG 3 - Good Health and Well-being
title_short Biochemical Characterization of the Copper Nitrite Reductase from Neisseria gonorrhoeae
title_full Biochemical Characterization of the Copper Nitrite Reductase from Neisseria gonorrhoeae
title_fullStr Biochemical Characterization of the Copper Nitrite Reductase from Neisseria gonorrhoeae
title_full_unstemmed Biochemical Characterization of the Copper Nitrite Reductase from Neisseria gonorrhoeae
title_sort Biochemical Characterization of the Copper Nitrite Reductase from Neisseria gonorrhoeae
author Barreiro, Daniela S.
author_facet Barreiro, Daniela S.
Oliveira, Ricardo N. S.
Pauleta, Sofia R.
author_role author
author2 Oliveira, Ricardo N. S.
Pauleta, Sofia R.
author2_role author
author
dc.contributor.none.fl_str_mv DQ - Departamento de Química
UCIBIO - Applied Molecular Biosciences Unit
RUN
dc.contributor.author.fl_str_mv Barreiro, Daniela S.
Oliveira, Ricardo N. S.
Pauleta, Sofia R.
dc.subject.por.fl_str_mv AniA
copper enzyme
copper nitrite reductase
hyperthermostable
Neisseria
nitrite reduction
spectroscopy
T1 copper center
T2 copper center
thermostability
Biochemistry
Molecular Biology
Biophysics
SDG 3 - Good Health and Well-being
topic AniA
copper enzyme
copper nitrite reductase
hyperthermostable
Neisseria
nitrite reduction
spectroscopy
T1 copper center
T2 copper center
thermostability
Biochemistry
Molecular Biology
Biophysics
SDG 3 - Good Health and Well-being
description Publisher Copyright: © 2023 by the authors.
publishDate 2023
dc.date.none.fl_str_mv 2023-09-18T22:13:43Z
2023-08-04
2023-08-04T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/157966
url http://hdl.handle.net/10362/157966
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2218-273X
PURE: 71829200
https://doi.org/10.3390/biom13081215
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 15
application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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