New potent membrane-targeting antibacterial peptides from viral capsid proteins

Detalhes bibliográficos
Autor(a) principal: Dias, Susana A.
Data de Publicação: 2017
Outros Autores: Freire, João M., Pérez-Peinado, Clara, Domingues, Marco M., Gaspar, Diana, Vale, Nuno, Gomes, Paula Gomes, Andreu, David, Henriques, Sónia T., Castanho, Miguel A. R. B., Veiga, Ana S.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/32531
Resumo: Copyright © 2017 Dias, Freire, Pérez-Peinado, Domingues, Gaspar, Vale, Gomes, Andreu, Henriques, Castanho and Veiga. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
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spelling New potent membrane-targeting antibacterial peptides from viral capsid proteinsAntimicrobial peptides (AMPs)Cell-penetrating peptides (CPPs)Minimum inhibitory concentration (MIC)Minimal bactericidal concentration (MBC)Membrane permeabilizationAtomic force microscopy (AFM)Copyright © 2017 Dias, Freire, Pérez-Peinado, Domingues, Gaspar, Vale, Gomes, Andreu, Henriques, Castanho and Veiga. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.The increasing prevalence of multidrug-resistant bacteria urges the development of new antibacterial agents. With a broad spectrum activity, antimicrobial peptides have been considered potential antibacterial drug leads. Using bioinformatic tools we have previously shown that viral structural proteins are a rich source for new bioactive peptide sequences, namely antimicrobial and cell-penetrating peptides. Here, we test the efficacy and mechanism of action of the most promising peptides among those previously identified against both Gram-positive and Gram-negative bacteria. Two cell-penetrating peptides, vCPP 0769 and vCPP 2319, have high antibacterial activity against Staphylococcus aureus, MRSA, Escherichia coli, and Pseudomonas aeruginosa, being thus multifunctional. The antibacterial mechanism of action of the two most active viral protein-derived peptides, vAMP 059 and vCPP 2319, was studied in detail. Both peptides act on both Gram-positive S. aureus and Gram-negative P. aeruginosa, with bacterial cell death occurring within minutes. Also, these peptides cause bacterial membrane permeabilization and damage of the bacterial envelope of P. aeruginosa cells. Overall, the results show that structural viral proteins are an abundant source for membrane-active peptides sequences with strong antibacterial properties.This work was supported by Fundação para a Ciência e a Tecnologia (FCT-MCTES, Portugal) projects PTDC/QEQMED/4412/2014 and UID/QUI/50006/2013, and by Marie Skłodowska-Curie Research and Innovation Staff Exchange (RISE): call H2020-MSCA-RISE-2014, Grant agreement 644167, 2015–2019. SD, JF, and DG acknowledge FCT for fellowships PD/BD/114425/2016, SFRH/BD/70423/2010, and SFRH/BPD/109010/2015, respectively, and MD for a grant (PTDC/BBB-BQB/3494/2014). ASV and NV acknowledge FCT for funding within the FCT Investigator Programme, IF/00803/2012 and IF/00092/2014, respectively. CP-P acknowledges financial support from the Spanish Ministry of Economy and Competitiveness, through grant AGL2014-52395-C2-2-R and the “María de Maeztu” Programme for Units of Excellence in R&D (MDM-2014-0370). PG acknowledges “Comissão de Coordenação e Desenvolvimento Regional do Norte (CCDR-N)/NORTE2020/Portugal 2020” for funding through project DESignBIOtechHealth (ref. Norte-01-0145-FEDER-000024). SH is the recipient of an Australian Research Council Future Fellowship (FT150100398).Frontiers MediaRepositório da Universidade de LisboaDias, Susana A.Freire, João M.Pérez-Peinado, ClaraDomingues, Marco M.Gaspar, DianaVale, NunoGomes, Paula GomesAndreu, DavidHenriques, Sónia T.Castanho, Miguel A. R. B.Veiga, Ana S.2018-04-02T11:26:53Z20172017-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/32531engFrontiers in Microbiology, May 2017 | Volume 8 | Article 7751664-302X10.3389/fmicb.2017.00775info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T16:26:53Zoai:repositorio.ul.pt:10451/32531Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:47:49.123239Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv New potent membrane-targeting antibacterial peptides from viral capsid proteins
title New potent membrane-targeting antibacterial peptides from viral capsid proteins
spellingShingle New potent membrane-targeting antibacterial peptides from viral capsid proteins
Dias, Susana A.
Antimicrobial peptides (AMPs)
Cell-penetrating peptides (CPPs)
Minimum inhibitory concentration (MIC)
Minimal bactericidal concentration (MBC)
Membrane permeabilization
Atomic force microscopy (AFM)
title_short New potent membrane-targeting antibacterial peptides from viral capsid proteins
title_full New potent membrane-targeting antibacterial peptides from viral capsid proteins
title_fullStr New potent membrane-targeting antibacterial peptides from viral capsid proteins
title_full_unstemmed New potent membrane-targeting antibacterial peptides from viral capsid proteins
title_sort New potent membrane-targeting antibacterial peptides from viral capsid proteins
author Dias, Susana A.
author_facet Dias, Susana A.
Freire, João M.
Pérez-Peinado, Clara
Domingues, Marco M.
Gaspar, Diana
Vale, Nuno
Gomes, Paula Gomes
Andreu, David
Henriques, Sónia T.
Castanho, Miguel A. R. B.
Veiga, Ana S.
author_role author
author2 Freire, João M.
Pérez-Peinado, Clara
Domingues, Marco M.
Gaspar, Diana
Vale, Nuno
Gomes, Paula Gomes
Andreu, David
Henriques, Sónia T.
Castanho, Miguel A. R. B.
Veiga, Ana S.
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Dias, Susana A.
Freire, João M.
Pérez-Peinado, Clara
Domingues, Marco M.
Gaspar, Diana
Vale, Nuno
Gomes, Paula Gomes
Andreu, David
Henriques, Sónia T.
Castanho, Miguel A. R. B.
Veiga, Ana S.
dc.subject.por.fl_str_mv Antimicrobial peptides (AMPs)
Cell-penetrating peptides (CPPs)
Minimum inhibitory concentration (MIC)
Minimal bactericidal concentration (MBC)
Membrane permeabilization
Atomic force microscopy (AFM)
topic Antimicrobial peptides (AMPs)
Cell-penetrating peptides (CPPs)
Minimum inhibitory concentration (MIC)
Minimal bactericidal concentration (MBC)
Membrane permeabilization
Atomic force microscopy (AFM)
description Copyright © 2017 Dias, Freire, Pérez-Peinado, Domingues, Gaspar, Vale, Gomes, Andreu, Henriques, Castanho and Veiga. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
publishDate 2017
dc.date.none.fl_str_mv 2017
2017-01-01T00:00:00Z
2018-04-02T11:26:53Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/32531
url http://hdl.handle.net/10451/32531
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Frontiers in Microbiology, May 2017 | Volume 8 | Article 775
1664-302X
10.3389/fmicb.2017.00775
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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