To what extent do fluorophores bias the biological activity of peptides? A practical approach using membrane-active peptides as models
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2020 |
| Outros Autores: | , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/10451/57440 |
Resumo: | Copyright © 2020 Cavaco, Pérez-Peinado, Valle, Silva, Correia, Andreu, Castanho and Neves. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms |
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oai:repositorio.ul.pt:10451/57440 |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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7160 |
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To what extent do fluorophores bias the biological activity of peptides? A practical approach using membrane-active peptides as modelsAnticancer peptidesBBB peptide shuttlesFluorescenceFluorophoreLabelingCopyright © 2020 Cavaco, Pérez-Peinado, Valle, Silva, Correia, Andreu, Castanho and Neves. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these termsThe characterization of biologically active peptides relies heavily on the study of their efficacy, toxicity, mechanism of action, cellular uptake, or intracellular location, using both in vitro and in vivo studies. These studies frequently depend on the use of fluorescence-based techniques. Since most peptides are not intrinsically fluorescent, they are conjugated to a fluorophore. The conjugation may interfere with peptide properties, thus biasing the results. The selection of the most suitable fluorophore is highly relevant. Here, a comprehensive study with blood–brain barrier (BBB) peptide shuttles (PepH3 and PepNeg) and antimicrobial peptides (AMPs) (vCPP2319 and Ctn[15-34]), tested as anticancer peptides (ACPs), having different fluorophores, namely 5(6)-carboxyfluorescein (CF), rhodamine B (RhB), quasar 570 (Q570), or tide fluor 3 (TF3) attached is presented. The goal is the evaluation of the impact of the selected fluorophores on peptide performance, applying routinely used techniques to assess cytotoxicity/toxicity, secondary structure, BBB translocation, and cellular internalization. Our results show that some fluorophores significantly modulate peptide activity when compared with unlabeled peptides, being more noticeable in hydrophobic and charged fluorophores. This study highlights the need for a careful experimental design for fluorescently labeled molecules, such as peptides.The authors thank the Portuguese Funding Agency, Fundação para a Ciência e a Tecnologia, FCT IP, for financial support (grants: PD/BD/128281/2017, PTDC/BBB-NAN/1578/2014); European Union’s Horizon 2020 Research and Innovation Programme under grant agreement No 828774; and “la Caixa” Banking Foundation under the project code HR17-00409.Frontiers MediaRepositório da Universidade de LisboaCavaco, MarcoPérez-Peinado, ClaraValle, JavierSilva, RúbenCorreia, João D. G.Castanho, Miguel A. R. B.Neves, VeraAndreu, David2023-05-17T14:31:19Z20202020-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/57440engFrontiers in Bioengineering and Biotechnology, September 2020 | Volume 8 | Article 55203510.3389/fbioe.2020.5520352296-4185info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T17:05:50Zoai:repositorio.ul.pt:10451/57440Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T22:07:55.242771Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
To what extent do fluorophores bias the biological activity of peptides? A practical approach using membrane-active peptides as models |
| title |
To what extent do fluorophores bias the biological activity of peptides? A practical approach using membrane-active peptides as models |
| spellingShingle |
To what extent do fluorophores bias the biological activity of peptides? A practical approach using membrane-active peptides as models Cavaco, Marco Anticancer peptides BBB peptide shuttles Fluorescence Fluorophore Labeling |
| title_short |
To what extent do fluorophores bias the biological activity of peptides? A practical approach using membrane-active peptides as models |
| title_full |
To what extent do fluorophores bias the biological activity of peptides? A practical approach using membrane-active peptides as models |
| title_fullStr |
To what extent do fluorophores bias the biological activity of peptides? A practical approach using membrane-active peptides as models |
| title_full_unstemmed |
To what extent do fluorophores bias the biological activity of peptides? A practical approach using membrane-active peptides as models |
| title_sort |
To what extent do fluorophores bias the biological activity of peptides? A practical approach using membrane-active peptides as models |
| author |
Cavaco, Marco |
| author_facet |
Cavaco, Marco Pérez-Peinado, Clara Valle, Javier Silva, Rúben Correia, João D. G. Castanho, Miguel A. R. B. Neves, Vera Andreu, David |
| author_role |
author |
| author2 |
Pérez-Peinado, Clara Valle, Javier Silva, Rúben Correia, João D. G. Castanho, Miguel A. R. B. Neves, Vera Andreu, David |
| author2_role |
author author author author author author author |
| dc.contributor.none.fl_str_mv |
Repositório da Universidade de Lisboa |
| dc.contributor.author.fl_str_mv |
Cavaco, Marco Pérez-Peinado, Clara Valle, Javier Silva, Rúben Correia, João D. G. Castanho, Miguel A. R. B. Neves, Vera Andreu, David |
| dc.subject.por.fl_str_mv |
Anticancer peptides BBB peptide shuttles Fluorescence Fluorophore Labeling |
| topic |
Anticancer peptides BBB peptide shuttles Fluorescence Fluorophore Labeling |
| description |
Copyright © 2020 Cavaco, Pérez-Peinado, Valle, Silva, Correia, Andreu, Castanho and Neves. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms |
| publishDate |
2020 |
| dc.date.none.fl_str_mv |
2020 2020-01-01T00:00:00Z 2023-05-17T14:31:19Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10451/57440 |
| url |
http://hdl.handle.net/10451/57440 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
Frontiers in Bioengineering and Biotechnology, September 2020 | Volume 8 | Article 552035 10.3389/fbioe.2020.552035 2296-4185 |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.publisher.none.fl_str_mv |
Frontiers Media |
| publisher.none.fl_str_mv |
Frontiers Media |
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reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
| instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
| instacron_str |
RCAAP |
| institution |
RCAAP |
| reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799134632773419008 |