Synthesis and biological evaluation of curcumin-based fluorophores

Detalhes bibliográficos
Autor(a) principal: Ferreira, Joana Raquel Mendes
Data de Publicação: 2021
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10773/30594
Resumo: Curcumin is a natural compound obtained from the rhizome of Curcuma longa. It has several properties of clinical interest such as antitumoral and antimicrobial properties and has been suggested as a photosensitizer in photodynamic therapy as an alternative therapy. Curcumin derivatives have also been used as fluorescent markers of protein aggregates in neurodegenerative diseases. In this work, a family of curcumin derivatives was synthesized, characterized and their photophysical properties were studied. The biological evaluation of these derivatives was studied in two cell models, the phytopathogenic fungus Fusarium oxysporum and the human cell line SH-SY5Y used in studies of neurodegenerative diseases, using cytotoxicity tests and fluorescence microscopy. The curcuminoids described were not toxic in the dark to the cell models under study and the location of some derivatives within the cells of the SH-SY5Y cell line is suggested.
id RCAP_f35953f7380768e2ce606509d8bb6bc6
oai_identifier_str oai:ria.ua.pt:10773/30594
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling Synthesis and biological evaluation of curcumin-based fluorophoresFluorescenceFluorophoreCurcuminBoron complexFusarium oxysporumSHSY5Y cell lineCell imagingFluorescence microscopyCurcumin is a natural compound obtained from the rhizome of Curcuma longa. It has several properties of clinical interest such as antitumoral and antimicrobial properties and has been suggested as a photosensitizer in photodynamic therapy as an alternative therapy. Curcumin derivatives have also been used as fluorescent markers of protein aggregates in neurodegenerative diseases. In this work, a family of curcumin derivatives was synthesized, characterized and their photophysical properties were studied. The biological evaluation of these derivatives was studied in two cell models, the phytopathogenic fungus Fusarium oxysporum and the human cell line SH-SY5Y used in studies of neurodegenerative diseases, using cytotoxicity tests and fluorescence microscopy. The curcuminoids described were not toxic in the dark to the cell models under study and the location of some derivatives within the cells of the SH-SY5Y cell line is suggested.A curcumina é um composto natural obtido do rizoma de Curcuma longa. Esta apresenta diversas propriedades clínicas, como propriedades antitumurais e antimicrobianas, e tem sido sugerida como fotossensibilizador em terapia fotodinâmica como terapia alternativa. Derivados de curcumina têm também sido usados como marcadores fluorescentes de agregados proteicos em doenças neurodegenerativas. Neste trabalho, uma família de derivados de curcumina foi sintetizada, caracterizada e foram estudadas as suas propriedades fotofísicas. A avaliação biológica destes derivados foi estudada em dois modelos celulares, o fungo fitopatogénico Fusarium oxysporum e a linha celular humana SH-SY5Y usada em estudos de doenças neurodegenerativas, recorrendo a testes de citotoxicidade e a microscopia de fluorescência. Os curcuminóides descritos não se mostraram tóxicos no escuro para os modelos celulares em estudo e ainda é sugerida a localização de alguns derivados no interior das células da linha celular SH-SY5Y.2023-01-27T00:00:00Z2021-01-26T00:00:00Z2021-01-26info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10773/30594engFerreira, Joana Raquel Mendesinfo:eu-repo/semantics/embargoedAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-02-22T11:59:05Zoai:ria.ua.pt:10773/30594Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:02:38.702578Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Synthesis and biological evaluation of curcumin-based fluorophores
title Synthesis and biological evaluation of curcumin-based fluorophores
spellingShingle Synthesis and biological evaluation of curcumin-based fluorophores
Ferreira, Joana Raquel Mendes
Fluorescence
Fluorophore
Curcumin
Boron complex
Fusarium oxysporum
SHSY5Y cell line
Cell imaging
Fluorescence microscopy
title_short Synthesis and biological evaluation of curcumin-based fluorophores
title_full Synthesis and biological evaluation of curcumin-based fluorophores
title_fullStr Synthesis and biological evaluation of curcumin-based fluorophores
title_full_unstemmed Synthesis and biological evaluation of curcumin-based fluorophores
title_sort Synthesis and biological evaluation of curcumin-based fluorophores
author Ferreira, Joana Raquel Mendes
author_facet Ferreira, Joana Raquel Mendes
author_role author
dc.contributor.author.fl_str_mv Ferreira, Joana Raquel Mendes
dc.subject.por.fl_str_mv Fluorescence
Fluorophore
Curcumin
Boron complex
Fusarium oxysporum
SHSY5Y cell line
Cell imaging
Fluorescence microscopy
topic Fluorescence
Fluorophore
Curcumin
Boron complex
Fusarium oxysporum
SHSY5Y cell line
Cell imaging
Fluorescence microscopy
description Curcumin is a natural compound obtained from the rhizome of Curcuma longa. It has several properties of clinical interest such as antitumoral and antimicrobial properties and has been suggested as a photosensitizer in photodynamic therapy as an alternative therapy. Curcumin derivatives have also been used as fluorescent markers of protein aggregates in neurodegenerative diseases. In this work, a family of curcumin derivatives was synthesized, characterized and their photophysical properties were studied. The biological evaluation of these derivatives was studied in two cell models, the phytopathogenic fungus Fusarium oxysporum and the human cell line SH-SY5Y used in studies of neurodegenerative diseases, using cytotoxicity tests and fluorescence microscopy. The curcuminoids described were not toxic in the dark to the cell models under study and the location of some derivatives within the cells of the SH-SY5Y cell line is suggested.
publishDate 2021
dc.date.none.fl_str_mv 2021-01-26T00:00:00Z
2021-01-26
2023-01-27T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10773/30594
url http://hdl.handle.net/10773/30594
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/embargoedAccess
eu_rights_str_mv embargoedAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799137681737777152

Registros relacionados