Identification of pesticides in forensic samples by mass spectrometry

Detalhes bibliográficos
Autor(a) principal: Ezeigwe, Francisca Chinemerem
Data de Publicação: 2021
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/120740
Resumo: The illegal use of pesticide formulations for the intentional/non-intentional poisoning of domestic/wild animals is, unfortunately, very common. This is a non-selective form of killing that affectsboth target and non-target species, endangers the environment and constitutes a risk in terms of publichealth. This study was undergone under the scope of a protocol established between the Laboratório de PolíciaCientífica da Polícia Judiciária (LPC/PJ) and Instituto Superior Técnico (IST) with the ultimate goal of establishing analytical protocols based on liquid chromatography low resolution tandem mass spectrometry (LC-MS/MS) analysis suitable for the identification of pesticides in forensic samples suspected of being involved in criminal animal poisoning. Towards this end, a data base of LC-MS/MS data of distinct pesticide standards was first constructed. The choice of pesticides included in the data base was based on the previous knowledge of their common use for illegal killing of domestic and wild animals in Portugal and Spain. Several samples provided by LPC/PJ were then analysed under the same conditions used for pesticide standards. This enabled the identification of pesticides in 10 forensic samples. Therefore, the methodology developed revealed to be suitable for the identification of pesticides in forensic samples.
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spelling Identification of pesticides in forensic samples by mass spectrometrypesticides identificationmass spectrometryLC-MS analysisanimal poisoningforensic samplesDomínio/Área Científica::Engenharia e Tecnologia::Engenharia QuímicaThe illegal use of pesticide formulations for the intentional/non-intentional poisoning of domestic/wild animals is, unfortunately, very common. This is a non-selective form of killing that affectsboth target and non-target species, endangers the environment and constitutes a risk in terms of publichealth. This study was undergone under the scope of a protocol established between the Laboratório de PolíciaCientífica da Polícia Judiciária (LPC/PJ) and Instituto Superior Técnico (IST) with the ultimate goal of establishing analytical protocols based on liquid chromatography low resolution tandem mass spectrometry (LC-MS/MS) analysis suitable for the identification of pesticides in forensic samples suspected of being involved in criminal animal poisoning. Towards this end, a data base of LC-MS/MS data of distinct pesticide standards was first constructed. The choice of pesticides included in the data base was based on the previous knowledge of their common use for illegal killing of domestic and wild animals in Portugal and Spain. Several samples provided by LPC/PJ were then analysed under the same conditions used for pesticide standards. This enabled the identification of pesticides in 10 forensic samples. Therefore, the methodology developed revealed to be suitable for the identification of pesticides in forensic samples.O uso ilegal de formulações de pesticidas para o envenenamento intencional ou não intencional deanimais domésticosou selvagens é, infelizmente, frequente. Esta é uma forma não seletiva de matar que afeta não só as espécies alvo como outras espécies. Esta prática põe em risco o meio ambiente econstitui um perigo em termos de saúde pública.. Este trabalho foi efetuado no âmbito de um protocolo estabelecido entre Laboratório de Polícia Científica da Polícia Judiciária (LPC/PJ) e o Instituto Superior Técnico (IST) e tem como objetivo estabelecer protocolos analíticos baseados na técnica de cromatografia líquida acoplada à espectrometria de massa de baixa resolução de tandem (LC-MS/MS) para a identificação de pesticidasem amostras forenses, de casos suspeitos de uso de pesticidas para o envenenamento de animais. Para tal, foi primeiro construída uma base de dados de LC-MS/MS com vários pesticidas padrão. A escolha dos pesticidas a incluir nesta base de dados teve como base o conhecimento prévio de seu uso comum num contexto de intoxicação de animais domésticos e selvagens em Portugal e Espanha. Várias amostras cedidas pelo LPC/PJ, que foram obtidas de casos onde havia a suspeita da utilizaçãode pesticidas para a intoxicação de animais, foram depois analisadas nas mesmas condições experimentais utilizadas para as amostras padrão. Esta estratégia revelou ser adequada para identificação de alguns pesticidas em amostras forenses, tendo-se identificado pesticidas em 10 das amostras forenses analisadas.Antunes, AlexandraCaldeira, Maria JoãoRUNEzeigwe, Francisca Chinemerem2021-07-09T15:35:01Z2021-052021-05-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10362/120740enginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:03:13Zoai:run.unl.pt:10362/120740Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:44:26.435588Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Identification of pesticides in forensic samples by mass spectrometry
title Identification of pesticides in forensic samples by mass spectrometry
spellingShingle Identification of pesticides in forensic samples by mass spectrometry
Ezeigwe, Francisca Chinemerem
pesticides identification
mass spectrometry
LC-MS analysis
animal poisoning
forensic samples
Domínio/Área Científica::Engenharia e Tecnologia::Engenharia Química
title_short Identification of pesticides in forensic samples by mass spectrometry
title_full Identification of pesticides in forensic samples by mass spectrometry
title_fullStr Identification of pesticides in forensic samples by mass spectrometry
title_full_unstemmed Identification of pesticides in forensic samples by mass spectrometry
title_sort Identification of pesticides in forensic samples by mass spectrometry
author Ezeigwe, Francisca Chinemerem
author_facet Ezeigwe, Francisca Chinemerem
author_role author
dc.contributor.none.fl_str_mv Antunes, Alexandra
Caldeira, Maria João
RUN
dc.contributor.author.fl_str_mv Ezeigwe, Francisca Chinemerem
dc.subject.por.fl_str_mv pesticides identification
mass spectrometry
LC-MS analysis
animal poisoning
forensic samples
Domínio/Área Científica::Engenharia e Tecnologia::Engenharia Química
topic pesticides identification
mass spectrometry
LC-MS analysis
animal poisoning
forensic samples
Domínio/Área Científica::Engenharia e Tecnologia::Engenharia Química
description The illegal use of pesticide formulations for the intentional/non-intentional poisoning of domestic/wild animals is, unfortunately, very common. This is a non-selective form of killing that affectsboth target and non-target species, endangers the environment and constitutes a risk in terms of publichealth. This study was undergone under the scope of a protocol established between the Laboratório de PolíciaCientífica da Polícia Judiciária (LPC/PJ) and Instituto Superior Técnico (IST) with the ultimate goal of establishing analytical protocols based on liquid chromatography low resolution tandem mass spectrometry (LC-MS/MS) analysis suitable for the identification of pesticides in forensic samples suspected of being involved in criminal animal poisoning. Towards this end, a data base of LC-MS/MS data of distinct pesticide standards was first constructed. The choice of pesticides included in the data base was based on the previous knowledge of their common use for illegal killing of domestic and wild animals in Portugal and Spain. Several samples provided by LPC/PJ were then analysed under the same conditions used for pesticide standards. This enabled the identification of pesticides in 10 forensic samples. Therefore, the methodology developed revealed to be suitable for the identification of pesticides in forensic samples.
publishDate 2021
dc.date.none.fl_str_mv 2021-07-09T15:35:01Z
2021-05
2021-05-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/120740
url http://hdl.handle.net/10362/120740
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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