Characterization of old portuguese grapevine varieties using microsatellite markers

Detalhes bibliográficos
Autor(a) principal: Alifragkis, Antonios D.
Data de Publicação: 2012
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.5/8614
Resumo: Mestrado Vinifera EuroMaster - Instituto Superior de Agronomia
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spelling Characterization of old portuguese grapevine varieties using microsatellite markersvitis viniferacultivar identificationmicrosatellitesDNA genotypingphylogenyMestrado Vinifera EuroMaster - Instituto Superior de AgronomiaGrapevine is an economically important crop cultivated all over the world. The long history of cultivation, the high ability of hybridization and the asexual propagation of selected plants has lead to the formation of numerous cultivars. The taxonomic classification of Vitaceae is inconvenient due to the high number of species within the Vitaceae family as well as the high polymorphism level of different cultivars. The classical ampelographic tools proved to be insufficient leading to an increasing number of synonymous and homonymous cultivars. Molecular methods for the identification and classification of grapevine were first used in 1970’s. Till then a plethora of new tools allows the correct and conclusive identification based on DNA analysis methods. The new techniques used can help to improve and optimize germplasm collections all over the world while also creating a database in a common language as a reference for the researchers. In the current study we dealt with the identification of 24 grapevine cultivars using 20 SSR markers. The marker VVIv37 failed to amplify due to a mistake in the primer sequence. All remaining markers gave satisfactory results allowing to identify 150 total alleles ranging from 3-13 alleles per locus. The observed Heterozygosity was 73%. We observed 5 cases of synonymy and 1 case of homonymy among the studied cultivars. The genetic distances of the cultivars lead to the designing of a phylogenetic tree revealing the relations among the cultivarsISA/ULDias, José Eduardo EirasLopes, Carlos Manuel AntunesRepositório da Universidade de LisboaAlifragkis, Antonios D.2015-12-31T01:30:11Z20122012-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10400.5/8614engAlifragkis. A.D. - Characterization of old portuguese grapevine varieties using microsatellite markers. Lisboa: ISA, 2012, 157 p.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-12-24T01:32:23Zoai:www.repository.utl.pt:10400.5/8614Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T16:55:34.707038Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Characterization of old portuguese grapevine varieties using microsatellite markers
title Characterization of old portuguese grapevine varieties using microsatellite markers
spellingShingle Characterization of old portuguese grapevine varieties using microsatellite markers
Alifragkis, Antonios D.
vitis vinifera
cultivar identification
microsatellites
DNA genotyping
phylogeny
title_short Characterization of old portuguese grapevine varieties using microsatellite markers
title_full Characterization of old portuguese grapevine varieties using microsatellite markers
title_fullStr Characterization of old portuguese grapevine varieties using microsatellite markers
title_full_unstemmed Characterization of old portuguese grapevine varieties using microsatellite markers
title_sort Characterization of old portuguese grapevine varieties using microsatellite markers
author Alifragkis, Antonios D.
author_facet Alifragkis, Antonios D.
author_role author
dc.contributor.none.fl_str_mv Dias, José Eduardo Eiras
Lopes, Carlos Manuel Antunes
Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Alifragkis, Antonios D.
dc.subject.por.fl_str_mv vitis vinifera
cultivar identification
microsatellites
DNA genotyping
phylogeny
topic vitis vinifera
cultivar identification
microsatellites
DNA genotyping
phylogeny
description Mestrado Vinifera EuroMaster - Instituto Superior de Agronomia
publishDate 2012
dc.date.none.fl_str_mv 2012
2012-01-01T00:00:00Z
2015-12-31T01:30:11Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.5/8614
url http://hdl.handle.net/10400.5/8614
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Alifragkis. A.D. - Characterization of old portuguese grapevine varieties using microsatellite markers. Lisboa: ISA, 2012, 157 p.
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv ISA/UL
publisher.none.fl_str_mv ISA/UL
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799131034591166464

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