A purification platform for antibodies and derived fragments using a de novo designed affinity adsorbent

Detalhes bibliográficos
Autor(a) principal: Matos, Manuel J. B.
Data de Publicação: 2021
Outros Autores: Trovão, Filipa, Gonçalves, João, Rothbauer, Ulrich, Freire, Mara G., Barbosa, Arménio M. J. B., Pina, Ana Sofia, Roque, Ana Cecília Afonso
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/141856
Resumo: Fundação para a Ciência e Tecnologia (FCT) -PTDC/BII-BIO/28878/2017; co-financed by ERDF under the PT2020 Partnership Agreement LISBOA-01-0145-FEDER-028878; PD/BD/128251/2016 from MIT-PT PhD Bioengineering Systems to MJBM; SFRH/BPD/112543/2015 to AJMB; Applied Molecular Biosciences Unit – UCIBIO (UIDP/04378/2020 ; UIDB/04378/2020); CICECO-Aveiro Institute of Materials (UIDB/50011/2020; UIDP/50011/2020), financed by national funds from FCT.
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spelling A purification platform for antibodies and derived fragments using a de novo designed affinity adsorbentAffinityAntibody fragments, synthetic ligandsAntibody purificationCaptureAnalytical ChemistryFiltration and SeparationFundação para a Ciência e Tecnologia (FCT) -PTDC/BII-BIO/28878/2017; co-financed by ERDF under the PT2020 Partnership Agreement LISBOA-01-0145-FEDER-028878; PD/BD/128251/2016 from MIT-PT PhD Bioengineering Systems to MJBM; SFRH/BPD/112543/2015 to AJMB; Applied Molecular Biosciences Unit – UCIBIO (UIDP/04378/2020 ; UIDB/04378/2020); CICECO-Aveiro Institute of Materials (UIDB/50011/2020; UIDP/50011/2020), financed by national funds from FCT.Antibody technologies are the most representative success-case in the biopharmaceutical industry. Widely available purification technologies fail in providing a dedicated universal purification platform that can accommodate antibodies structural diversity, namely antibodies from non-human sources, as chicken IgY, and antigen-binding fragments. In this work, we took inspiration from natural and engineered antibody-binding ligands, to rationally design affinity adsorbents able to capture full-length antibodies and fragments. The one-pot Petasis and Ugi combinatorial reactions were sequentially employed to rapidly generate a library of putative solid-phase adsorbents. The best performing adsorbent yielded a single-step recovery, under mild conditions, of human and chicken whole antibodies, antigen-binding fragments and engineered single-domain antibodies from different complex feedstocks. Due to its simple preparation, the lead antibody adsorbent finds broad applicability as a universal purification platform to increase the availability of antibody technologies in research and development.DQ - Departamento de QuímicaUCIBIO - Applied Molecular Biosciences UnitRUNMatos, Manuel J. B.Trovão, FilipaGonçalves, JoãoRothbauer, UlrichFreire, Mara G.Barbosa, Arménio M. J. B.Pina, Ana SofiaRoque, Ana Cecília Afonso2023-02-19T01:31:50Z2021-06-152021-06-15T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/141856eng1383-5866PURE: 28483655https://doi.org/10.1016/j.seppur.2021.118476info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:19:11Zoai:run.unl.pt:10362/141856Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:50:04.658487Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv A purification platform for antibodies and derived fragments using a de novo designed affinity adsorbent
title A purification platform for antibodies and derived fragments using a de novo designed affinity adsorbent
spellingShingle A purification platform for antibodies and derived fragments using a de novo designed affinity adsorbent
Matos, Manuel J. B.
Affinity
Antibody fragments, synthetic ligands
Antibody purification
Capture
Analytical Chemistry
Filtration and Separation
title_short A purification platform for antibodies and derived fragments using a de novo designed affinity adsorbent
title_full A purification platform for antibodies and derived fragments using a de novo designed affinity adsorbent
title_fullStr A purification platform for antibodies and derived fragments using a de novo designed affinity adsorbent
title_full_unstemmed A purification platform for antibodies and derived fragments using a de novo designed affinity adsorbent
title_sort A purification platform for antibodies and derived fragments using a de novo designed affinity adsorbent
author Matos, Manuel J. B.
author_facet Matos, Manuel J. B.
Trovão, Filipa
Gonçalves, João
Rothbauer, Ulrich
Freire, Mara G.
Barbosa, Arménio M. J. B.
Pina, Ana Sofia
Roque, Ana Cecília Afonso
author_role author
author2 Trovão, Filipa
Gonçalves, João
Rothbauer, Ulrich
Freire, Mara G.
Barbosa, Arménio M. J. B.
Pina, Ana Sofia
Roque, Ana Cecília Afonso
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv DQ - Departamento de Química
UCIBIO - Applied Molecular Biosciences Unit
RUN
dc.contributor.author.fl_str_mv Matos, Manuel J. B.
Trovão, Filipa
Gonçalves, João
Rothbauer, Ulrich
Freire, Mara G.
Barbosa, Arménio M. J. B.
Pina, Ana Sofia
Roque, Ana Cecília Afonso
dc.subject.por.fl_str_mv Affinity
Antibody fragments, synthetic ligands
Antibody purification
Capture
Analytical Chemistry
Filtration and Separation
topic Affinity
Antibody fragments, synthetic ligands
Antibody purification
Capture
Analytical Chemistry
Filtration and Separation
description Fundação para a Ciência e Tecnologia (FCT) -PTDC/BII-BIO/28878/2017; co-financed by ERDF under the PT2020 Partnership Agreement LISBOA-01-0145-FEDER-028878; PD/BD/128251/2016 from MIT-PT PhD Bioengineering Systems to MJBM; SFRH/BPD/112543/2015 to AJMB; Applied Molecular Biosciences Unit – UCIBIO (UIDP/04378/2020 ; UIDB/04378/2020); CICECO-Aveiro Institute of Materials (UIDB/50011/2020; UIDP/50011/2020), financed by national funds from FCT.
publishDate 2021
dc.date.none.fl_str_mv 2021-06-15
2021-06-15T00:00:00Z
2023-02-19T01:31:50Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/141856
url http://hdl.handle.net/10362/141856
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1383-5866
PURE: 28483655
https://doi.org/10.1016/j.seppur.2021.118476
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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