A purification platform for antibodies and derived fragments using a de novo designed affinity adsorbent
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10362/141856 |
Resumo: | Fundação para a Ciência e Tecnologia (FCT) -PTDC/BII-BIO/28878/2017; co-financed by ERDF under the PT2020 Partnership Agreement LISBOA-01-0145-FEDER-028878; PD/BD/128251/2016 from MIT-PT PhD Bioengineering Systems to MJBM; SFRH/BPD/112543/2015 to AJMB; Applied Molecular Biosciences Unit – UCIBIO (UIDP/04378/2020 ; UIDB/04378/2020); CICECO-Aveiro Institute of Materials (UIDB/50011/2020; UIDP/50011/2020), financed by national funds from FCT. |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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7160 |
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A purification platform for antibodies and derived fragments using a de novo designed affinity adsorbentAffinityAntibody fragments, synthetic ligandsAntibody purificationCaptureAnalytical ChemistryFiltration and SeparationFundação para a Ciência e Tecnologia (FCT) -PTDC/BII-BIO/28878/2017; co-financed by ERDF under the PT2020 Partnership Agreement LISBOA-01-0145-FEDER-028878; PD/BD/128251/2016 from MIT-PT PhD Bioengineering Systems to MJBM; SFRH/BPD/112543/2015 to AJMB; Applied Molecular Biosciences Unit – UCIBIO (UIDP/04378/2020 ; UIDB/04378/2020); CICECO-Aveiro Institute of Materials (UIDB/50011/2020; UIDP/50011/2020), financed by national funds from FCT.Antibody technologies are the most representative success-case in the biopharmaceutical industry. Widely available purification technologies fail in providing a dedicated universal purification platform that can accommodate antibodies structural diversity, namely antibodies from non-human sources, as chicken IgY, and antigen-binding fragments. In this work, we took inspiration from natural and engineered antibody-binding ligands, to rationally design affinity adsorbents able to capture full-length antibodies and fragments. The one-pot Petasis and Ugi combinatorial reactions were sequentially employed to rapidly generate a library of putative solid-phase adsorbents. The best performing adsorbent yielded a single-step recovery, under mild conditions, of human and chicken whole antibodies, antigen-binding fragments and engineered single-domain antibodies from different complex feedstocks. Due to its simple preparation, the lead antibody adsorbent finds broad applicability as a universal purification platform to increase the availability of antibody technologies in research and development.DQ - Departamento de QuímicaUCIBIO - Applied Molecular Biosciences UnitRUNMatos, Manuel J. B.Trovão, FilipaGonçalves, JoãoRothbauer, UlrichFreire, Mara G.Barbosa, Arménio M. J. B.Pina, Ana SofiaRoque, Ana Cecília Afonso2023-02-19T01:31:50Z2021-06-152021-06-15T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/141856eng1383-5866PURE: 28483655https://doi.org/10.1016/j.seppur.2021.118476info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-05-22T18:03:27Zoai:run.unl.pt:10362/141856Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-05-22T18:03:27Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
A purification platform for antibodies and derived fragments using a de novo designed affinity adsorbent |
title |
A purification platform for antibodies and derived fragments using a de novo designed affinity adsorbent |
spellingShingle |
A purification platform for antibodies and derived fragments using a de novo designed affinity adsorbent Matos, Manuel J. B. Affinity Antibody fragments, synthetic ligands Antibody purification Capture Analytical Chemistry Filtration and Separation |
title_short |
A purification platform for antibodies and derived fragments using a de novo designed affinity adsorbent |
title_full |
A purification platform for antibodies and derived fragments using a de novo designed affinity adsorbent |
title_fullStr |
A purification platform for antibodies and derived fragments using a de novo designed affinity adsorbent |
title_full_unstemmed |
A purification platform for antibodies and derived fragments using a de novo designed affinity adsorbent |
title_sort |
A purification platform for antibodies and derived fragments using a de novo designed affinity adsorbent |
author |
Matos, Manuel J. B. |
author_facet |
Matos, Manuel J. B. Trovão, Filipa Gonçalves, João Rothbauer, Ulrich Freire, Mara G. Barbosa, Arménio M. J. B. Pina, Ana Sofia Roque, Ana Cecília Afonso |
author_role |
author |
author2 |
Trovão, Filipa Gonçalves, João Rothbauer, Ulrich Freire, Mara G. Barbosa, Arménio M. J. B. Pina, Ana Sofia Roque, Ana Cecília Afonso |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
DQ - Departamento de Química UCIBIO - Applied Molecular Biosciences Unit RUN |
dc.contributor.author.fl_str_mv |
Matos, Manuel J. B. Trovão, Filipa Gonçalves, João Rothbauer, Ulrich Freire, Mara G. Barbosa, Arménio M. J. B. Pina, Ana Sofia Roque, Ana Cecília Afonso |
dc.subject.por.fl_str_mv |
Affinity Antibody fragments, synthetic ligands Antibody purification Capture Analytical Chemistry Filtration and Separation |
topic |
Affinity Antibody fragments, synthetic ligands Antibody purification Capture Analytical Chemistry Filtration and Separation |
description |
Fundação para a Ciência e Tecnologia (FCT) -PTDC/BII-BIO/28878/2017; co-financed by ERDF under the PT2020 Partnership Agreement LISBOA-01-0145-FEDER-028878; PD/BD/128251/2016 from MIT-PT PhD Bioengineering Systems to MJBM; SFRH/BPD/112543/2015 to AJMB; Applied Molecular Biosciences Unit – UCIBIO (UIDP/04378/2020 ; UIDB/04378/2020); CICECO-Aveiro Institute of Materials (UIDB/50011/2020; UIDP/50011/2020), financed by national funds from FCT. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-15 2021-06-15T00:00:00Z 2023-02-19T01:31:50Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/141856 |
url |
http://hdl.handle.net/10362/141856 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
1383-5866 PURE: 28483655 https://doi.org/10.1016/j.seppur.2021.118476 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
mluisa.alvim@gmail.com |
_version_ |
1817545874698403840 |