Size fractionation by slalom chromatography and hydrodynamic chromatography
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10198/7011 |
Resumo: | Hydrodynamic chromatography, also called separation by flow, is based on the use of the parabolic flow profile occurring in open capillaries or in the pores from a column filled with non-porous particles. The hydrodynamic chromatography separation medium, if any, is much simpler than that from size exclusion chromatography (porous particles), the former technique being used in the size-fractionation of many colloids and macromolecules. The transition between hydrodynamic chromatography (obtained using low flow rates) and slalom chromatography (obtained using high flow rates) was reported in studies related with the migration behaviour of proteins, plasmid DNAs and double stranded DNAs. Slalom chromatography is mainly applied in the size-fractionation of large double stranded DNA fragments and may compete with electrophoretic techniques. In the present paper it is discussed the main patents, applications, advantages and drawbacks related to hydrodynamic chromatography and slalom chromatography, as well as some strategies that may improve the performance of these simple size-fractionation techniques. |
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Size fractionation by slalom chromatography and hydrodynamic chromatographySeparation by flowHydrodynamic chromatographySlalom chromatographyParticle size distributionSynthetic polymersBiopolymersStarchDNAHydrodynamic chromatography, also called separation by flow, is based on the use of the parabolic flow profile occurring in open capillaries or in the pores from a column filled with non-porous particles. The hydrodynamic chromatography separation medium, if any, is much simpler than that from size exclusion chromatography (porous particles), the former technique being used in the size-fractionation of many colloids and macromolecules. The transition between hydrodynamic chromatography (obtained using low flow rates) and slalom chromatography (obtained using high flow rates) was reported in studies related with the migration behaviour of proteins, plasmid DNAs and double stranded DNAs. Slalom chromatography is mainly applied in the size-fractionation of large double stranded DNA fragments and may compete with electrophoretic techniques. In the present paper it is discussed the main patents, applications, advantages and drawbacks related to hydrodynamic chromatography and slalom chromatography, as well as some strategies that may improve the performance of these simple size-fractionation techniques.Bentham Science PublishersBiblioteca Digital do IPBDias, Ricardo P.2012-06-05T12:58:48Z20082008-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10198/7011engDias, Ricardo P. (2008). Size fractionation by slalom chromatography and hydrodynamic chromatography. Recent Patents on Engineering. ISSN 1872-2121. 2:2, p. 95-1031872-2121info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-21T10:17:28Zoai:bibliotecadigital.ipb.pt:10198/7011Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T22:58:53.559911Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Size fractionation by slalom chromatography and hydrodynamic chromatography |
title |
Size fractionation by slalom chromatography and hydrodynamic chromatography |
spellingShingle |
Size fractionation by slalom chromatography and hydrodynamic chromatography Dias, Ricardo P. Separation by flow Hydrodynamic chromatography Slalom chromatography Particle size distribution Synthetic polymers Biopolymers Starch DNA |
title_short |
Size fractionation by slalom chromatography and hydrodynamic chromatography |
title_full |
Size fractionation by slalom chromatography and hydrodynamic chromatography |
title_fullStr |
Size fractionation by slalom chromatography and hydrodynamic chromatography |
title_full_unstemmed |
Size fractionation by slalom chromatography and hydrodynamic chromatography |
title_sort |
Size fractionation by slalom chromatography and hydrodynamic chromatography |
author |
Dias, Ricardo P. |
author_facet |
Dias, Ricardo P. |
author_role |
author |
dc.contributor.none.fl_str_mv |
Biblioteca Digital do IPB |
dc.contributor.author.fl_str_mv |
Dias, Ricardo P. |
dc.subject.por.fl_str_mv |
Separation by flow Hydrodynamic chromatography Slalom chromatography Particle size distribution Synthetic polymers Biopolymers Starch DNA |
topic |
Separation by flow Hydrodynamic chromatography Slalom chromatography Particle size distribution Synthetic polymers Biopolymers Starch DNA |
description |
Hydrodynamic chromatography, also called separation by flow, is based on the use of the parabolic flow profile occurring in open capillaries or in the pores from a column filled with non-porous particles. The hydrodynamic chromatography separation medium, if any, is much simpler than that from size exclusion chromatography (porous particles), the former technique being used in the size-fractionation of many colloids and macromolecules. The transition between hydrodynamic chromatography (obtained using low flow rates) and slalom chromatography (obtained using high flow rates) was reported in studies related with the migration behaviour of proteins, plasmid DNAs and double stranded DNAs. Slalom chromatography is mainly applied in the size-fractionation of large double stranded DNA fragments and may compete with electrophoretic techniques. In the present paper it is discussed the main patents, applications, advantages and drawbacks related to hydrodynamic chromatography and slalom chromatography, as well as some strategies that may improve the performance of these simple size-fractionation techniques. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008 2008-01-01T00:00:00Z 2012-06-05T12:58:48Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10198/7011 |
url |
http://hdl.handle.net/10198/7011 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Dias, Ricardo P. (2008). Size fractionation by slalom chromatography and hydrodynamic chromatography. Recent Patents on Engineering. ISSN 1872-2121. 2:2, p. 95-103 1872-2121 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Bentham Science Publishers |
publisher.none.fl_str_mv |
Bentham Science Publishers |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1817552813466583040 |