Size fractionation by slalom chromatography and hydrodynamic chromatography

Detalhes bibliográficos
Autor(a) principal: Dias, Ricardo P.
Data de Publicação: 2008
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10198/7011
Resumo: Hydrodynamic chromatography, also called separation by flow, is based on the use of the parabolic flow profile occurring in open capillaries or in the pores from a column filled with non-porous particles. The hydrodynamic chromatography separation medium, if any, is much simpler than that from size exclusion chromatography (porous particles), the former technique being used in the size-fractionation of many colloids and macromolecules. The transition between hydrodynamic chromatography (obtained using low flow rates) and slalom chromatography (obtained using high flow rates) was reported in studies related with the migration behaviour of proteins, plasmid DNAs and double stranded DNAs. Slalom chromatography is mainly applied in the size-fractionation of large double stranded DNA fragments and may compete with electrophoretic techniques. In the present paper it is discussed the main patents, applications, advantages and drawbacks related to hydrodynamic chromatography and slalom chromatography, as well as some strategies that may improve the performance of these simple size-fractionation techniques.
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spelling Size fractionation by slalom chromatography and hydrodynamic chromatographySeparation by flowHydrodynamic chromatographySlalom chromatographyParticle size distributionSynthetic polymersBiopolymersStarchDNAHydrodynamic chromatography, also called separation by flow, is based on the use of the parabolic flow profile occurring in open capillaries or in the pores from a column filled with non-porous particles. The hydrodynamic chromatography separation medium, if any, is much simpler than that from size exclusion chromatography (porous particles), the former technique being used in the size-fractionation of many colloids and macromolecules. The transition between hydrodynamic chromatography (obtained using low flow rates) and slalom chromatography (obtained using high flow rates) was reported in studies related with the migration behaviour of proteins, plasmid DNAs and double stranded DNAs. Slalom chromatography is mainly applied in the size-fractionation of large double stranded DNA fragments and may compete with electrophoretic techniques. In the present paper it is discussed the main patents, applications, advantages and drawbacks related to hydrodynamic chromatography and slalom chromatography, as well as some strategies that may improve the performance of these simple size-fractionation techniques.Bentham Science PublishersBiblioteca Digital do IPBDias, Ricardo P.2012-06-05T12:58:48Z20082008-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10198/7011engDias, Ricardo P. (2008). Size fractionation by slalom chromatography and hydrodynamic chromatography. Recent Patents on Engineering. ISSN 1872-2121. 2:2, p. 95-1031872-2121info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-21T10:17:28Zoai:bibliotecadigital.ipb.pt:10198/7011Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T22:58:53.559911Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Size fractionation by slalom chromatography and hydrodynamic chromatography
title Size fractionation by slalom chromatography and hydrodynamic chromatography
spellingShingle Size fractionation by slalom chromatography and hydrodynamic chromatography
Dias, Ricardo P.
Separation by flow
Hydrodynamic chromatography
Slalom chromatography
Particle size distribution
Synthetic polymers
Biopolymers
Starch
DNA
title_short Size fractionation by slalom chromatography and hydrodynamic chromatography
title_full Size fractionation by slalom chromatography and hydrodynamic chromatography
title_fullStr Size fractionation by slalom chromatography and hydrodynamic chromatography
title_full_unstemmed Size fractionation by slalom chromatography and hydrodynamic chromatography
title_sort Size fractionation by slalom chromatography and hydrodynamic chromatography
author Dias, Ricardo P.
author_facet Dias, Ricardo P.
author_role author
dc.contributor.none.fl_str_mv Biblioteca Digital do IPB
dc.contributor.author.fl_str_mv Dias, Ricardo P.
dc.subject.por.fl_str_mv Separation by flow
Hydrodynamic chromatography
Slalom chromatography
Particle size distribution
Synthetic polymers
Biopolymers
Starch
DNA
topic Separation by flow
Hydrodynamic chromatography
Slalom chromatography
Particle size distribution
Synthetic polymers
Biopolymers
Starch
DNA
description Hydrodynamic chromatography, also called separation by flow, is based on the use of the parabolic flow profile occurring in open capillaries or in the pores from a column filled with non-porous particles. The hydrodynamic chromatography separation medium, if any, is much simpler than that from size exclusion chromatography (porous particles), the former technique being used in the size-fractionation of many colloids and macromolecules. The transition between hydrodynamic chromatography (obtained using low flow rates) and slalom chromatography (obtained using high flow rates) was reported in studies related with the migration behaviour of proteins, plasmid DNAs and double stranded DNAs. Slalom chromatography is mainly applied in the size-fractionation of large double stranded DNA fragments and may compete with electrophoretic techniques. In the present paper it is discussed the main patents, applications, advantages and drawbacks related to hydrodynamic chromatography and slalom chromatography, as well as some strategies that may improve the performance of these simple size-fractionation techniques.
publishDate 2008
dc.date.none.fl_str_mv 2008
2008-01-01T00:00:00Z
2012-06-05T12:58:48Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10198/7011
url http://hdl.handle.net/10198/7011
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Dias, Ricardo P. (2008). Size fractionation by slalom chromatography and hydrodynamic chromatography. Recent Patents on Engineering. ISSN 1872-2121. 2:2, p. 95-103
1872-2121
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Bentham Science Publishers
publisher.none.fl_str_mv Bentham Science Publishers
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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