Purification of a synthetic pterocarpanquinone by countercurrent chromatography

Detalhes bibliográficos
Autor(a) principal: Costa,Fernanda das Neves
Data de Publicação: 2012
Outros Autores: Silva,Alcides José M. da, Netto,Chaquip Daher, Domingos,Jorge L. de Oliveira, Costa,Paulo Roberto R., Leitão,Gilda G.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Journal of the Brazilian Chemical Society (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532012000600016
Resumo: Countercurrent chromatography (CCC) was employed as a useful, fast and economic alternative to conventional chromatography techniques for the purification of a synthetic pterocarpanquinone, LQB-118. The separation was performed in a two-step CCC with the solvent system hexane-chloroform-methanol-water 2:1.5:5:2 in both steps. Traditional purification of these reaction products by silica gel column chromatography demanded a large amount of solvent and time, besides allowing the irreversible adsorption of the compound in the column. The use of ¹H NMR for the calculation of K D of target compound is proposed as an alternative for HPLC measurements.
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spelling Purification of a synthetic pterocarpanquinone by countercurrent chromatographycountercurrent chromatographypterocarpanquinoneflash chromatographydistribution coefficient (K D)Countercurrent chromatography (CCC) was employed as a useful, fast and economic alternative to conventional chromatography techniques for the purification of a synthetic pterocarpanquinone, LQB-118. The separation was performed in a two-step CCC with the solvent system hexane-chloroform-methanol-water 2:1.5:5:2 in both steps. Traditional purification of these reaction products by silica gel column chromatography demanded a large amount of solvent and time, besides allowing the irreversible adsorption of the compound in the column. The use of ¹H NMR for the calculation of K D of target compound is proposed as an alternative for HPLC measurements.Sociedade Brasileira de Química2012-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532012000600016Journal of the Brazilian Chemical Society v.23 n.6 2012reponame:Journal of the Brazilian Chemical Society (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.1590/S0103-50532012000600016info:eu-repo/semantics/openAccessCosta,Fernanda das NevesSilva,Alcides José M. daNetto,Chaquip DaherDomingos,Jorge L. de OliveiraCosta,Paulo Roberto R.Leitão,Gilda G.eng2012-07-13T00:00:00Zoai:scielo:S0103-50532012000600016Revistahttp://jbcs.sbq.org.brONGhttps://old.scielo.br/oai/scielo-oai.php||office@jbcs.sbq.org.br1678-47900103-5053opendoar:2012-07-13T00:00Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)false
dc.title.none.fl_str_mv Purification of a synthetic pterocarpanquinone by countercurrent chromatography
title Purification of a synthetic pterocarpanquinone by countercurrent chromatography
spellingShingle Purification of a synthetic pterocarpanquinone by countercurrent chromatography
Costa,Fernanda das Neves
countercurrent chromatography
pterocarpanquinone
flash chromatography
distribution coefficient (K D)
title_short Purification of a synthetic pterocarpanquinone by countercurrent chromatography
title_full Purification of a synthetic pterocarpanquinone by countercurrent chromatography
title_fullStr Purification of a synthetic pterocarpanquinone by countercurrent chromatography
title_full_unstemmed Purification of a synthetic pterocarpanquinone by countercurrent chromatography
title_sort Purification of a synthetic pterocarpanquinone by countercurrent chromatography
author Costa,Fernanda das Neves
author_facet Costa,Fernanda das Neves
Silva,Alcides José M. da
Netto,Chaquip Daher
Domingos,Jorge L. de Oliveira
Costa,Paulo Roberto R.
Leitão,Gilda G.
author_role author
author2 Silva,Alcides José M. da
Netto,Chaquip Daher
Domingos,Jorge L. de Oliveira
Costa,Paulo Roberto R.
Leitão,Gilda G.
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Costa,Fernanda das Neves
Silva,Alcides José M. da
Netto,Chaquip Daher
Domingos,Jorge L. de Oliveira
Costa,Paulo Roberto R.
Leitão,Gilda G.
dc.subject.por.fl_str_mv countercurrent chromatography
pterocarpanquinone
flash chromatography
distribution coefficient (K D)
topic countercurrent chromatography
pterocarpanquinone
flash chromatography
distribution coefficient (K D)
description Countercurrent chromatography (CCC) was employed as a useful, fast and economic alternative to conventional chromatography techniques for the purification of a synthetic pterocarpanquinone, LQB-118. The separation was performed in a two-step CCC with the solvent system hexane-chloroform-methanol-water 2:1.5:5:2 in both steps. Traditional purification of these reaction products by silica gel column chromatography demanded a large amount of solvent and time, besides allowing the irreversible adsorption of the compound in the column. The use of ¹H NMR for the calculation of K D of target compound is proposed as an alternative for HPLC measurements.
publishDate 2012
dc.date.none.fl_str_mv 2012-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532012000600016
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532012000600016
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0103-50532012000600016
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Química
publisher.none.fl_str_mv Sociedade Brasileira de Química
dc.source.none.fl_str_mv Journal of the Brazilian Chemical Society v.23 n.6 2012
reponame:Journal of the Brazilian Chemical Society (Online)
instname:Sociedade Brasileira de Química (SBQ)
instacron:SBQ
instname_str Sociedade Brasileira de Química (SBQ)
instacron_str SBQ
institution SBQ
reponame_str Journal of the Brazilian Chemical Society (Online)
collection Journal of the Brazilian Chemical Society (Online)
repository.name.fl_str_mv Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)
repository.mail.fl_str_mv ||office@jbcs.sbq.org.br
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