A new method to predict genotoxic effects based on serum molecular profile

Detalhes bibliográficos
Autor(a) principal: Araújo, Rúben
Data de Publicação: 2021
Outros Autores: Ramalhete, Luís, Paz, Hélder, Ladeira, Carina, Calado, Cecília
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.21/13125
Resumo: IDI&CA/IPL/2017/GenTox/ESTeSL
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spelling A new method to predict genotoxic effects based on serum molecular profileBiomonitoringGenotoxicitySerumCBMNFTIR spectroscopyIDI&CA/IPL/2017/GenTox/ESTeSLIDI&CA/IPL/2017/GenTox/ESTeSLIt is critical to developing new methods to assess genotoxic effects in human biomonitoring since conventional methods are usually laborious, time-consuming, and expensive. It is aimed to evaluate if the analysis of a drop of serum by Fourier Transform Infrared spectroscopy, allows assessing genotoxic effects in occupational exposure to cytostatic drugs in hospital professionals, as obtained by the lymphocyte cytokinesis-block micronucleus assay. It was considered peripheral blood from hospital professionals exposed to cytostatic drugs (n = 22) and from a non-exposed group (n = 36). It was observed that workers occupationally exposed presented a higher number of micronuclei (p < 0.05) in lymphocytes, in relation to the non-exposed group. The serum Fourier Transform Infrared spectra from exposed workers presented diverse different peaks (p < 0.01) in relation to the non-exposed group. The hierarchical cluster analysis of serum spectra separated serum samples of the exposed group from the non-exposed group with 61% sensitivity and 88% specificity. A support vector machine model of serum spectra enables the prediction of exposure with high accuracy (0.91), precision (0.89), sensitivity (0.86), F1 score (0.87), and AUC (0.96). Therefore, Fourier Transform Infrared spectroscopic analysis of a drop of serum enabled to predict in a rapid and simple mode the genotoxic effects of cytostatic drugs. The method presents therefore potential for high-dimension screening of exposure to genotoxic substances, due to its simplicity and rapid setup mode.ElsevierRCIPLAraújo, RúbenRamalhete, LuísPaz, HélderLadeira, CarinaCalado, Cecília2021-032021-03-01T00:00:00Z2024-03-19T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.21/13125engAraújo R, Ramalhete L, Paz H, Ladeira C, Calado CR. A new method to predict genotoxic effects based on serum molecular profile. Spectrochim Acta A Mol Biomol Spectroscop. 2021;255:119680.10.1016/j.saa.2021.119680info:eu-repo/semantics/embargoedAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-08-03T10:07:20Zoai:repositorio.ipl.pt:10400.21/13125Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:21:07.755018Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv A new method to predict genotoxic effects based on serum molecular profile
title A new method to predict genotoxic effects based on serum molecular profile
spellingShingle A new method to predict genotoxic effects based on serum molecular profile
Araújo, Rúben
Biomonitoring
Genotoxicity
Serum
CBMN
FTIR spectroscopy
IDI&CA/IPL/2017/GenTox/ESTeSL
title_short A new method to predict genotoxic effects based on serum molecular profile
title_full A new method to predict genotoxic effects based on serum molecular profile
title_fullStr A new method to predict genotoxic effects based on serum molecular profile
title_full_unstemmed A new method to predict genotoxic effects based on serum molecular profile
title_sort A new method to predict genotoxic effects based on serum molecular profile
author Araújo, Rúben
author_facet Araújo, Rúben
Ramalhete, Luís
Paz, Hélder
Ladeira, Carina
Calado, Cecília
author_role author
author2 Ramalhete, Luís
Paz, Hélder
Ladeira, Carina
Calado, Cecília
author2_role author
author
author
author
dc.contributor.none.fl_str_mv RCIPL
dc.contributor.author.fl_str_mv Araújo, Rúben
Ramalhete, Luís
Paz, Hélder
Ladeira, Carina
Calado, Cecília
dc.subject.por.fl_str_mv Biomonitoring
Genotoxicity
Serum
CBMN
FTIR spectroscopy
IDI&CA/IPL/2017/GenTox/ESTeSL
topic Biomonitoring
Genotoxicity
Serum
CBMN
FTIR spectroscopy
IDI&CA/IPL/2017/GenTox/ESTeSL
description IDI&CA/IPL/2017/GenTox/ESTeSL
publishDate 2021
dc.date.none.fl_str_mv 2021-03
2021-03-01T00:00:00Z
2024-03-19T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.21/13125
url http://hdl.handle.net/10400.21/13125
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Araújo R, Ramalhete L, Paz H, Ladeira C, Calado CR. A new method to predict genotoxic effects based on serum molecular profile. Spectrochim Acta A Mol Biomol Spectroscop. 2021;255:119680.
10.1016/j.saa.2021.119680
dc.rights.driver.fl_str_mv info:eu-repo/semantics/embargoedAccess
eu_rights_str_mv embargoedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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