Semipermeable capsules wrapping a multifunctional and self-regulated co-culture microenvironment for osteogenic differentiation
Autor(a) principal: | |
---|---|
Data de Publicação: | 2016 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://hdl.handle.net/1822/40913 |
Resumo: | A new concept of semipermeable reservoirs containing co-cultures of cells and supporting microparticles is presented, inspired by the multi-phenotypic cellular environment of bone. Based on the deconstruction of the â stem cell nicheâ , the developed capsules are designed to drive a self-regulated osteogenesis. PLLA microparticles functionalized with collagen I, and a co-culture of adipose stem (ASCs) and endothelial (ECs) cells are immobilized in spherical liquified capsules. The capsules are coated with multilayers of poly(L-lysine), alginate, and chitosan nano-assembled through layer-by-layer. Capsules encapsulating ASCs alone or in a co-culture with ECs are cultured in endothelial medium with or without osteogenic differentiation factors. Results show that osteogenesis is enhanced by the co-encapsulation, which occurs even in the absence of differentiation factors. These findings are supported by an increased ALP activity and matrix mineralization, osteopontin detection, and the up regulation of BMP-2, RUNX2 and BSP. The liquified co-capsules also act as a VEGF and BMP-2 cytokines release system. The proposed liquified capsules might be a valuable injectable self-regulated system for bone regeneration employing highly translational cell sources. |
id |
RCAP_d13c0b06b44a5ec6ebdecf01aeca21c3 |
---|---|
oai_identifier_str |
oai:repositorium.sdum.uminho.pt:1822/40913 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
|
spelling |
Semipermeable capsules wrapping a multifunctional and self-regulated co-culture microenvironment for osteogenic differentiationBioinspired materialsBiomedical engineeringBiomineralizationGrowth factor signallingStem-cell researchScience & TechnologyA new concept of semipermeable reservoirs containing co-cultures of cells and supporting microparticles is presented, inspired by the multi-phenotypic cellular environment of bone. Based on the deconstruction of the â stem cell nicheâ , the developed capsules are designed to drive a self-regulated osteogenesis. PLLA microparticles functionalized with collagen I, and a co-culture of adipose stem (ASCs) and endothelial (ECs) cells are immobilized in spherical liquified capsules. The capsules are coated with multilayers of poly(L-lysine), alginate, and chitosan nano-assembled through layer-by-layer. Capsules encapsulating ASCs alone or in a co-culture with ECs are cultured in endothelial medium with or without osteogenic differentiation factors. Results show that osteogenesis is enhanced by the co-encapsulation, which occurs even in the absence of differentiation factors. These findings are supported by an increased ALP activity and matrix mineralization, osteopontin detection, and the up regulation of BMP-2, RUNX2 and BSP. The liquified co-capsules also act as a VEGF and BMP-2 cytokines release system. The proposed liquified capsules might be a valuable injectable self-regulated system for bone regeneration employing highly translational cell sources.The authors acknowledge the financial support by the Portuguese Foundation for Science and Technology (FCT) through the Ph.D. (SFRH/BD/69529/2010-Clara R. Correia) and the Post-doc grants (SFRH/BPD/96611/2013- Mariana T. Cerqueira), and the funding of RL3-TECT-NORTE-01-0124-FEDER-000020 for Rogério P. Pirraco. This work was also supported by European Research Council grant agreement ERC-2014-ADG-669858 for project ATLAS.Nature Publishing GroupUniversidade do MinhoCorreia, Clara R.Pirraco, Rogério P.Cerqueira, Mariana TeixeiraMarques, A. P.Reis, R. L.Mano, J. F.20162016-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/40913engCorreia C. R., Pirraco R. P., Cerqueira M. T., Marques A. P., Reis R. L., Mano J. F. Semipermeable Capsules Wrapping a Multifunctional and Self-regulated Co-culture Microenvironment for Osteogenic Differentiation, Scientific Reports, Vol. 6, pp. 21883, doi:10.1038/srep21883, 20162045-232210.1038/srep2188326905619http://www.nature.com/articles/srep21883info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:45:32ZPortal AgregadorONG |
dc.title.none.fl_str_mv |
Semipermeable capsules wrapping a multifunctional and self-regulated co-culture microenvironment for osteogenic differentiation |
title |
Semipermeable capsules wrapping a multifunctional and self-regulated co-culture microenvironment for osteogenic differentiation |
spellingShingle |
Semipermeable capsules wrapping a multifunctional and self-regulated co-culture microenvironment for osteogenic differentiation Correia, Clara R. Bioinspired materials Biomedical engineering Biomineralization Growth factor signalling Stem-cell research Science & Technology |
title_short |
Semipermeable capsules wrapping a multifunctional and self-regulated co-culture microenvironment for osteogenic differentiation |
title_full |
Semipermeable capsules wrapping a multifunctional and self-regulated co-culture microenvironment for osteogenic differentiation |
title_fullStr |
Semipermeable capsules wrapping a multifunctional and self-regulated co-culture microenvironment for osteogenic differentiation |
title_full_unstemmed |
Semipermeable capsules wrapping a multifunctional and self-regulated co-culture microenvironment for osteogenic differentiation |
title_sort |
Semipermeable capsules wrapping a multifunctional and self-regulated co-culture microenvironment for osteogenic differentiation |
author |
Correia, Clara R. |
author_facet |
Correia, Clara R. Pirraco, Rogério P. Cerqueira, Mariana Teixeira Marques, A. P. Reis, R. L. Mano, J. F. |
author_role |
author |
author2 |
Pirraco, Rogério P. Cerqueira, Mariana Teixeira Marques, A. P. Reis, R. L. Mano, J. F. |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Correia, Clara R. Pirraco, Rogério P. Cerqueira, Mariana Teixeira Marques, A. P. Reis, R. L. Mano, J. F. |
dc.subject.por.fl_str_mv |
Bioinspired materials Biomedical engineering Biomineralization Growth factor signalling Stem-cell research Science & Technology |
topic |
Bioinspired materials Biomedical engineering Biomineralization Growth factor signalling Stem-cell research Science & Technology |
description |
A new concept of semipermeable reservoirs containing co-cultures of cells and supporting microparticles is presented, inspired by the multi-phenotypic cellular environment of bone. Based on the deconstruction of the â stem cell nicheâ , the developed capsules are designed to drive a self-regulated osteogenesis. PLLA microparticles functionalized with collagen I, and a co-culture of adipose stem (ASCs) and endothelial (ECs) cells are immobilized in spherical liquified capsules. The capsules are coated with multilayers of poly(L-lysine), alginate, and chitosan nano-assembled through layer-by-layer. Capsules encapsulating ASCs alone or in a co-culture with ECs are cultured in endothelial medium with or without osteogenic differentiation factors. Results show that osteogenesis is enhanced by the co-encapsulation, which occurs even in the absence of differentiation factors. These findings are supported by an increased ALP activity and matrix mineralization, osteopontin detection, and the up regulation of BMP-2, RUNX2 and BSP. The liquified co-capsules also act as a VEGF and BMP-2 cytokines release system. The proposed liquified capsules might be a valuable injectable self-regulated system for bone regeneration employing highly translational cell sources. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016 2016-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://hdl.handle.net/1822/40913 |
url |
https://hdl.handle.net/1822/40913 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Correia C. R., Pirraco R. P., Cerqueira M. T., Marques A. P., Reis R. L., Mano J. F. Semipermeable Capsules Wrapping a Multifunctional and Self-regulated Co-culture Microenvironment for Osteogenic Differentiation, Scientific Reports, Vol. 6, pp. 21883, doi:10.1038/srep21883, 2016 2045-2322 10.1038/srep21883 26905619 http://www.nature.com/articles/srep21883 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Nature Publishing Group |
publisher.none.fl_str_mv |
Nature Publishing Group |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
|
repository.mail.fl_str_mv |
|
_version_ |
1777303836718792704 |