Effect of taxifolin and epigallocatechin-3-gallate on biomineralization potential of stem cells from dental apical papilla
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.archoralbio.2022.105413 http://hdl.handle.net/11449/223730 |
Resumo: | Objective: Considering the variety of pharmacological activities and the potential to mediate biomineralization, the flavonoids taxifolin and epigallocatechin-3-gallate (EGCG) could be explored as biomolecules in scaffolds for regenerative endodontic procedures. The aim of this study was to evaluate the effect of taxifolin and EGCG on the cell viability, differentiation, and expression of biomineralization markers in stem cells from the apical papilla. Design: Stem cells from the apical papilla were exposed to single or continuous treatments with taxifolin at 200, 100 and 50 µM and EGCG at 50, 25 and 12.5 µM for 48 h, 8 and 14 days, in regular or mineralizing media. Cell viability, alkaline phosphatase activity and calcium deposition were analyzed using resazurin, p-nitrophenylphosphate and alizarin-based assays. Results: None of the flavonoid groups affected cell viability at 48 h, however at 8 and 14 days, Taxifolin 200 µM and EGCG 50 µM were cytotoxic. Cells did not express alkaline phosphatase activity when grown in regular medium, even in the presence of flavonoids. Alkaline phosphatase activity and biomineralization potential were higher in cells treated with Taxifolin 50 µM and EGCG 12.5 µM. Conclusion: Taxifolin and EGCG exhibited a concentration, time, and therapeutic mode dependent bioactivity on stem cells from the apical papilla. Both flavonoids at the lower concentrations tested exhibited cytocompatibility and increased expression of mineralization markers in the presence of mineralizing agents. |
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Effect of taxifolin and epigallocatechin-3-gallate on biomineralization potential of stem cells from dental apical papillaApical papillaBiomineralizationFlavonoidsMesenchymal stem cellsObjective: Considering the variety of pharmacological activities and the potential to mediate biomineralization, the flavonoids taxifolin and epigallocatechin-3-gallate (EGCG) could be explored as biomolecules in scaffolds for regenerative endodontic procedures. The aim of this study was to evaluate the effect of taxifolin and EGCG on the cell viability, differentiation, and expression of biomineralization markers in stem cells from the apical papilla. Design: Stem cells from the apical papilla were exposed to single or continuous treatments with taxifolin at 200, 100 and 50 µM and EGCG at 50, 25 and 12.5 µM for 48 h, 8 and 14 days, in regular or mineralizing media. Cell viability, alkaline phosphatase activity and calcium deposition were analyzed using resazurin, p-nitrophenylphosphate and alizarin-based assays. Results: None of the flavonoid groups affected cell viability at 48 h, however at 8 and 14 days, Taxifolin 200 µM and EGCG 50 µM were cytotoxic. Cells did not express alkaline phosphatase activity when grown in regular medium, even in the presence of flavonoids. Alkaline phosphatase activity and biomineralization potential were higher in cells treated with Taxifolin 50 µM and EGCG 12.5 µM. Conclusion: Taxifolin and EGCG exhibited a concentration, time, and therapeutic mode dependent bioactivity on stem cells from the apical papilla. Both flavonoids at the lower concentrations tested exhibited cytocompatibility and increased expression of mineralization markers in the presence of mineralizing agents.University of TorontoNatural Sciences and Engineering Research Council of CanadaCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Department of Preventive and Restorative Dentistry School of Dentistry São Paulo State University (UNESP), SPDental Research Institute Faculty of Dentistry University of TorontoDepartment of Preventive and Restorative Dentistry School of Dentistry São Paulo State University (UNESP), SPNatural Sciences and Engineering Research Council of Canada: #2018- 06489CAPES: 88881.171490/2018-01Universidade Estadual Paulista (UNESP)University of TorontoDuque, Cristiane [UNESP]Hussein, HebatullahBortolatto, JanainaPrakki, AnuradhaKishen, Anil2022-04-28T19:52:47Z2022-04-28T19:52:47Z2022-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.archoralbio.2022.105413Archives of Oral Biology, v. 138.1879-15060003-9969http://hdl.handle.net/11449/22373010.1016/j.archoralbio.2022.1054132-s2.0-85127267083Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengArchives of Oral Biologyinfo:eu-repo/semantics/openAccess2022-04-28T19:52:47Zoai:repositorio.unesp.br:11449/223730Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:01:13.152237Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Effect of taxifolin and epigallocatechin-3-gallate on biomineralization potential of stem cells from dental apical papilla |
title |
Effect of taxifolin and epigallocatechin-3-gallate on biomineralization potential of stem cells from dental apical papilla |
spellingShingle |
Effect of taxifolin and epigallocatechin-3-gallate on biomineralization potential of stem cells from dental apical papilla Duque, Cristiane [UNESP] Apical papilla Biomineralization Flavonoids Mesenchymal stem cells |
title_short |
Effect of taxifolin and epigallocatechin-3-gallate on biomineralization potential of stem cells from dental apical papilla |
title_full |
Effect of taxifolin and epigallocatechin-3-gallate on biomineralization potential of stem cells from dental apical papilla |
title_fullStr |
Effect of taxifolin and epigallocatechin-3-gallate on biomineralization potential of stem cells from dental apical papilla |
title_full_unstemmed |
Effect of taxifolin and epigallocatechin-3-gallate on biomineralization potential of stem cells from dental apical papilla |
title_sort |
Effect of taxifolin and epigallocatechin-3-gallate on biomineralization potential of stem cells from dental apical papilla |
author |
Duque, Cristiane [UNESP] |
author_facet |
Duque, Cristiane [UNESP] Hussein, Hebatullah Bortolatto, Janaina Prakki, Anuradha Kishen, Anil |
author_role |
author |
author2 |
Hussein, Hebatullah Bortolatto, Janaina Prakki, Anuradha Kishen, Anil |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) University of Toronto |
dc.contributor.author.fl_str_mv |
Duque, Cristiane [UNESP] Hussein, Hebatullah Bortolatto, Janaina Prakki, Anuradha Kishen, Anil |
dc.subject.por.fl_str_mv |
Apical papilla Biomineralization Flavonoids Mesenchymal stem cells |
topic |
Apical papilla Biomineralization Flavonoids Mesenchymal stem cells |
description |
Objective: Considering the variety of pharmacological activities and the potential to mediate biomineralization, the flavonoids taxifolin and epigallocatechin-3-gallate (EGCG) could be explored as biomolecules in scaffolds for regenerative endodontic procedures. The aim of this study was to evaluate the effect of taxifolin and EGCG on the cell viability, differentiation, and expression of biomineralization markers in stem cells from the apical papilla. Design: Stem cells from the apical papilla were exposed to single or continuous treatments with taxifolin at 200, 100 and 50 µM and EGCG at 50, 25 and 12.5 µM for 48 h, 8 and 14 days, in regular or mineralizing media. Cell viability, alkaline phosphatase activity and calcium deposition were analyzed using resazurin, p-nitrophenylphosphate and alizarin-based assays. Results: None of the flavonoid groups affected cell viability at 48 h, however at 8 and 14 days, Taxifolin 200 µM and EGCG 50 µM were cytotoxic. Cells did not express alkaline phosphatase activity when grown in regular medium, even in the presence of flavonoids. Alkaline phosphatase activity and biomineralization potential were higher in cells treated with Taxifolin 50 µM and EGCG 12.5 µM. Conclusion: Taxifolin and EGCG exhibited a concentration, time, and therapeutic mode dependent bioactivity on stem cells from the apical papilla. Both flavonoids at the lower concentrations tested exhibited cytocompatibility and increased expression of mineralization markers in the presence of mineralizing agents. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-04-28T19:52:47Z 2022-04-28T19:52:47Z 2022-06-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.archoralbio.2022.105413 Archives of Oral Biology, v. 138. 1879-1506 0003-9969 http://hdl.handle.net/11449/223730 10.1016/j.archoralbio.2022.105413 2-s2.0-85127267083 |
url |
http://dx.doi.org/10.1016/j.archoralbio.2022.105413 http://hdl.handle.net/11449/223730 |
identifier_str_mv |
Archives of Oral Biology, v. 138. 1879-1506 0003-9969 10.1016/j.archoralbio.2022.105413 2-s2.0-85127267083 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Archives of Oral Biology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808128447633948672 |