Effect of taxifolin and epigallocatechin-3-gallate on biomineralization potential of stem cells from dental apical papilla

Detalhes bibliográficos
Autor(a) principal: Duque, Cristiane [UNESP]
Data de Publicação: 2022
Outros Autores: Hussein, Hebatullah, Bortolatto, Janaina, Prakki, Anuradha, Kishen, Anil
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.archoralbio.2022.105413
http://hdl.handle.net/11449/223730
Resumo: Objective: Considering the variety of pharmacological activities and the potential to mediate biomineralization, the flavonoids taxifolin and epigallocatechin-3-gallate (EGCG) could be explored as biomolecules in scaffolds for regenerative endodontic procedures. The aim of this study was to evaluate the effect of taxifolin and EGCG on the cell viability, differentiation, and expression of biomineralization markers in stem cells from the apical papilla. Design: Stem cells from the apical papilla were exposed to single or continuous treatments with taxifolin at 200, 100 and 50 µM and EGCG at 50, 25 and 12.5 µM for 48 h, 8 and 14 days, in regular or mineralizing media. Cell viability, alkaline phosphatase activity and calcium deposition were analyzed using resazurin, p-nitrophenylphosphate and alizarin-based assays. Results: None of the flavonoid groups affected cell viability at 48 h, however at 8 and 14 days, Taxifolin 200 µM and EGCG 50 µM were cytotoxic. Cells did not express alkaline phosphatase activity when grown in regular medium, even in the presence of flavonoids. Alkaline phosphatase activity and biomineralization potential were higher in cells treated with Taxifolin 50 µM and EGCG 12.5 µM. Conclusion: Taxifolin and EGCG exhibited a concentration, time, and therapeutic mode dependent bioactivity on stem cells from the apical papilla. Both flavonoids at the lower concentrations tested exhibited cytocompatibility and increased expression of mineralization markers in the presence of mineralizing agents.
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spelling Effect of taxifolin and epigallocatechin-3-gallate on biomineralization potential of stem cells from dental apical papillaApical papillaBiomineralizationFlavonoidsMesenchymal stem cellsObjective: Considering the variety of pharmacological activities and the potential to mediate biomineralization, the flavonoids taxifolin and epigallocatechin-3-gallate (EGCG) could be explored as biomolecules in scaffolds for regenerative endodontic procedures. The aim of this study was to evaluate the effect of taxifolin and EGCG on the cell viability, differentiation, and expression of biomineralization markers in stem cells from the apical papilla. Design: Stem cells from the apical papilla were exposed to single or continuous treatments with taxifolin at 200, 100 and 50 µM and EGCG at 50, 25 and 12.5 µM for 48 h, 8 and 14 days, in regular or mineralizing media. Cell viability, alkaline phosphatase activity and calcium deposition were analyzed using resazurin, p-nitrophenylphosphate and alizarin-based assays. Results: None of the flavonoid groups affected cell viability at 48 h, however at 8 and 14 days, Taxifolin 200 µM and EGCG 50 µM were cytotoxic. Cells did not express alkaline phosphatase activity when grown in regular medium, even in the presence of flavonoids. Alkaline phosphatase activity and biomineralization potential were higher in cells treated with Taxifolin 50 µM and EGCG 12.5 µM. Conclusion: Taxifolin and EGCG exhibited a concentration, time, and therapeutic mode dependent bioactivity on stem cells from the apical papilla. Both flavonoids at the lower concentrations tested exhibited cytocompatibility and increased expression of mineralization markers in the presence of mineralizing agents.University of TorontoNatural Sciences and Engineering Research Council of CanadaCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Department of Preventive and Restorative Dentistry School of Dentistry São Paulo State University (UNESP), SPDental Research Institute Faculty of Dentistry University of TorontoDepartment of Preventive and Restorative Dentistry School of Dentistry São Paulo State University (UNESP), SPNatural Sciences and Engineering Research Council of Canada: #2018- 06489CAPES: 88881.171490/2018-01Universidade Estadual Paulista (UNESP)University of TorontoDuque, Cristiane [UNESP]Hussein, HebatullahBortolatto, JanainaPrakki, AnuradhaKishen, Anil2022-04-28T19:52:47Z2022-04-28T19:52:47Z2022-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.archoralbio.2022.105413Archives of Oral Biology, v. 138.1879-15060003-9969http://hdl.handle.net/11449/22373010.1016/j.archoralbio.2022.1054132-s2.0-85127267083Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengArchives of Oral Biologyinfo:eu-repo/semantics/openAccess2022-04-28T19:52:47Zoai:repositorio.unesp.br:11449/223730Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:01:13.152237Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Effect of taxifolin and epigallocatechin-3-gallate on biomineralization potential of stem cells from dental apical papilla
title Effect of taxifolin and epigallocatechin-3-gallate on biomineralization potential of stem cells from dental apical papilla
spellingShingle Effect of taxifolin and epigallocatechin-3-gallate on biomineralization potential of stem cells from dental apical papilla
Duque, Cristiane [UNESP]
Apical papilla
Biomineralization
Flavonoids
Mesenchymal stem cells
title_short Effect of taxifolin and epigallocatechin-3-gallate on biomineralization potential of stem cells from dental apical papilla
title_full Effect of taxifolin and epigallocatechin-3-gallate on biomineralization potential of stem cells from dental apical papilla
title_fullStr Effect of taxifolin and epigallocatechin-3-gallate on biomineralization potential of stem cells from dental apical papilla
title_full_unstemmed Effect of taxifolin and epigallocatechin-3-gallate on biomineralization potential of stem cells from dental apical papilla
title_sort Effect of taxifolin and epigallocatechin-3-gallate on biomineralization potential of stem cells from dental apical papilla
author Duque, Cristiane [UNESP]
author_facet Duque, Cristiane [UNESP]
Hussein, Hebatullah
Bortolatto, Janaina
Prakki, Anuradha
Kishen, Anil
author_role author
author2 Hussein, Hebatullah
Bortolatto, Janaina
Prakki, Anuradha
Kishen, Anil
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
University of Toronto
dc.contributor.author.fl_str_mv Duque, Cristiane [UNESP]
Hussein, Hebatullah
Bortolatto, Janaina
Prakki, Anuradha
Kishen, Anil
dc.subject.por.fl_str_mv Apical papilla
Biomineralization
Flavonoids
Mesenchymal stem cells
topic Apical papilla
Biomineralization
Flavonoids
Mesenchymal stem cells
description Objective: Considering the variety of pharmacological activities and the potential to mediate biomineralization, the flavonoids taxifolin and epigallocatechin-3-gallate (EGCG) could be explored as biomolecules in scaffolds for regenerative endodontic procedures. The aim of this study was to evaluate the effect of taxifolin and EGCG on the cell viability, differentiation, and expression of biomineralization markers in stem cells from the apical papilla. Design: Stem cells from the apical papilla were exposed to single or continuous treatments with taxifolin at 200, 100 and 50 µM and EGCG at 50, 25 and 12.5 µM for 48 h, 8 and 14 days, in regular or mineralizing media. Cell viability, alkaline phosphatase activity and calcium deposition were analyzed using resazurin, p-nitrophenylphosphate and alizarin-based assays. Results: None of the flavonoid groups affected cell viability at 48 h, however at 8 and 14 days, Taxifolin 200 µM and EGCG 50 µM were cytotoxic. Cells did not express alkaline phosphatase activity when grown in regular medium, even in the presence of flavonoids. Alkaline phosphatase activity and biomineralization potential were higher in cells treated with Taxifolin 50 µM and EGCG 12.5 µM. Conclusion: Taxifolin and EGCG exhibited a concentration, time, and therapeutic mode dependent bioactivity on stem cells from the apical papilla. Both flavonoids at the lower concentrations tested exhibited cytocompatibility and increased expression of mineralization markers in the presence of mineralizing agents.
publishDate 2022
dc.date.none.fl_str_mv 2022-04-28T19:52:47Z
2022-04-28T19:52:47Z
2022-06-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.archoralbio.2022.105413
Archives of Oral Biology, v. 138.
1879-1506
0003-9969
http://hdl.handle.net/11449/223730
10.1016/j.archoralbio.2022.105413
2-s2.0-85127267083
url http://dx.doi.org/10.1016/j.archoralbio.2022.105413
http://hdl.handle.net/11449/223730
identifier_str_mv Archives of Oral Biology, v. 138.
1879-1506
0003-9969
10.1016/j.archoralbio.2022.105413
2-s2.0-85127267083
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Archives of Oral Biology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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