In vitro antimicrobial activity of extracts and essential oils of Cinnamomum, Salvia, and Menthaspp. against foodborne pathogens: a meta‐analysis study

Detalhes bibliográficos
Autor(a) principal: Ezzaky, Youssef
Data de Publicação: 2023
Outros Autores: Elmoslih, Abdelkhaleq, Silva, Beatriz Nunes, Bonilla‐Luque, Olga M., Possas, Arícia, Valero, Antonio, Cadavez, Vasco, Gonzales-Barron, Ursula, Achemchem, Fouad
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10198/29342
Resumo: Genetic analysis of the honey bee spermathecal content can be particularly useful to provide an estimate of the genetic diversity and purity of the surrounding populations. Here we compared the concentration and quality of DNA extracted from queen spermatheca using four commercial kits to determine the best method to obtain DNA suitable for single nucleotide polymorphism genotyping by next-generation sequencing. The four kits were tested with different adjustments in the lysis incubation time, use of RNA-carrier, elution conditions and number of re-elutions. Only the use of QIAamp DNA Microkit with 3 h of lysis incubation, the addition of RNA-carrier and multiple re-elutions produced a DNA concentration over the required threshold.
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spelling In vitro antimicrobial activity of extracts and essential oils of Cinnamomum, Salvia, and Menthaspp. against foodborne pathogens: a meta‐analysis studyHoney beeSpermathecaGenomic DNA isolationDNA extractionGenetic analysis of the honey bee spermathecal content can be particularly useful to provide an estimate of the genetic diversity and purity of the surrounding populations. Here we compared the concentration and quality of DNA extracted from queen spermatheca using four commercial kits to determine the best method to obtain DNA suitable for single nucleotide polymorphism genotyping by next-generation sequencing. The four kits were tested with different adjustments in the lysis incubation time, use of RNA-carrier, elution conditions and number of re-elutions. Only the use of QIAamp DNA Microkit with 3 h of lysis incubation, the addition of RNA-carrier and multiple re-elutions produced a DNA concentration over the required threshold.Contributions of Jakob Wegener and Eduard Musin were supported by funds of the Federal Ministry of Food and Agriculture based on a decision of the Parliament of the Federal Republic of Germany via the Federal Office for Agriculture and Food, grant number 2818BM040. Dora Henriques was supported by BEEHAPPY (POCI-01-0145- FEDER-029871; FCT and COMPETE/QREN/EU). Ana R. Lopes and Carlos A. Yadró were supported by Fundaçãoo para a Ciência e a Tecnologia (FCT) grants SFRH/BD/143627/2019 and 2021.06948.BD, respectively. FCT provided financial support by national funds (FCT/MCTES) to CIMO (UIDB/00690/2020 and UIDP/00690/2020) and SusTEC (LA/P/0007/2021).WileyBiblioteca Digital do IPBEzzaky, YoussefElmoslih, AbdelkhaleqSilva, Beatriz NunesBonilla‐Luque, Olga M.Possas, AríciaValero, AntonioCadavez, VascoGonzales-Barron, UrsulaAchemchem, Fouad2024-01-24T12:28:24Z20232023-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10198/29342engEzzaky, Youssef; Elmoslih, Abdelkhaleq; Silva, Beatriz Nunes; Bonilla‐Luque, Olga M.; Possas, Arícia; Valero, Antonio; Cadavez, Vasco; Gonzales‐Barron, Ursula; Achemchem, Fouad (2023). In vitro antimicrobial activity of extracts and essential oils of Cinnamomum, Salvia, and Menthaspp. against foodborne pathogens: a meta‐analysis study. Comprehensive Reviews in Food Science and Food Safety. ISSN 1541-4337. 22:6, p. 1-41541-433710.1111/1541-4337.13232info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-31T01:19:12Zoai:bibliotecadigital.ipb.pt:10198/29342Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:58:56.354066Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv In vitro antimicrobial activity of extracts and essential oils of Cinnamomum, Salvia, and Menthaspp. against foodborne pathogens: a meta‐analysis study
title In vitro antimicrobial activity of extracts and essential oils of Cinnamomum, Salvia, and Menthaspp. against foodborne pathogens: a meta‐analysis study
spellingShingle In vitro antimicrobial activity of extracts and essential oils of Cinnamomum, Salvia, and Menthaspp. against foodborne pathogens: a meta‐analysis study
Ezzaky, Youssef
Honey bee
Spermatheca
Genomic DNA isolation
DNA extraction
title_short In vitro antimicrobial activity of extracts and essential oils of Cinnamomum, Salvia, and Menthaspp. against foodborne pathogens: a meta‐analysis study
title_full In vitro antimicrobial activity of extracts and essential oils of Cinnamomum, Salvia, and Menthaspp. against foodborne pathogens: a meta‐analysis study
title_fullStr In vitro antimicrobial activity of extracts and essential oils of Cinnamomum, Salvia, and Menthaspp. against foodborne pathogens: a meta‐analysis study
title_full_unstemmed In vitro antimicrobial activity of extracts and essential oils of Cinnamomum, Salvia, and Menthaspp. against foodborne pathogens: a meta‐analysis study
title_sort In vitro antimicrobial activity of extracts and essential oils of Cinnamomum, Salvia, and Menthaspp. against foodborne pathogens: a meta‐analysis study
author Ezzaky, Youssef
author_facet Ezzaky, Youssef
Elmoslih, Abdelkhaleq
Silva, Beatriz Nunes
Bonilla‐Luque, Olga M.
Possas, Arícia
Valero, Antonio
Cadavez, Vasco
Gonzales-Barron, Ursula
Achemchem, Fouad
author_role author
author2 Elmoslih, Abdelkhaleq
Silva, Beatriz Nunes
Bonilla‐Luque, Olga M.
Possas, Arícia
Valero, Antonio
Cadavez, Vasco
Gonzales-Barron, Ursula
Achemchem, Fouad
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Biblioteca Digital do IPB
dc.contributor.author.fl_str_mv Ezzaky, Youssef
Elmoslih, Abdelkhaleq
Silva, Beatriz Nunes
Bonilla‐Luque, Olga M.
Possas, Arícia
Valero, Antonio
Cadavez, Vasco
Gonzales-Barron, Ursula
Achemchem, Fouad
dc.subject.por.fl_str_mv Honey bee
Spermatheca
Genomic DNA isolation
DNA extraction
topic Honey bee
Spermatheca
Genomic DNA isolation
DNA extraction
description Genetic analysis of the honey bee spermathecal content can be particularly useful to provide an estimate of the genetic diversity and purity of the surrounding populations. Here we compared the concentration and quality of DNA extracted from queen spermatheca using four commercial kits to determine the best method to obtain DNA suitable for single nucleotide polymorphism genotyping by next-generation sequencing. The four kits were tested with different adjustments in the lysis incubation time, use of RNA-carrier, elution conditions and number of re-elutions. Only the use of QIAamp DNA Microkit with 3 h of lysis incubation, the addition of RNA-carrier and multiple re-elutions produced a DNA concentration over the required threshold.
publishDate 2023
dc.date.none.fl_str_mv 2023
2023-01-01T00:00:00Z
2024-01-24T12:28:24Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10198/29342
url http://hdl.handle.net/10198/29342
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Ezzaky, Youssef; Elmoslih, Abdelkhaleq; Silva, Beatriz Nunes; Bonilla‐Luque, Olga M.; Possas, Arícia; Valero, Antonio; Cadavez, Vasco; Gonzales‐Barron, Ursula; Achemchem, Fouad (2023). In vitro antimicrobial activity of extracts and essential oils of Cinnamomum, Salvia, and Menthaspp. against foodborne pathogens: a meta‐analysis study. Comprehensive Reviews in Food Science and Food Safety. ISSN 1541-4337. 22:6, p. 1-4
1541-4337
10.1111/1541-4337.13232
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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