An alternative method for Staphylococcus aureus DNA isolation

• Autor(a) principal: Chapaval,L.
• Data de Publicação: 2008
• Outros Autores: Moon,D.H., Gomes,J.E., Duarte,F.R., Tsai,S.M.
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Arquivo brasileiro de medicina veterinária e zootecnia (Online)
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352008000200004
• topic: Staphylococcus aureus; genomic DNA; DNA isolation

This study describes a rapid procedure for the isolation of genomic DNA from Staphylococcus aureus that yielded a good amount of high quality DNA for the amplification of staphylococcal enterotoxins genes (A, B, C, D, and E) and the TSST-1 gene as well as enzymatic restriction (HaeIII) from environmental isolates. With this method, it was possible to detect these genes in a sample containing as little as 10(5) cells with positive PCR reactions obtained from approximately 10pg of DNA in a final reaction volume of 25µl.