In Vivo High-Content Evaluation of Three-Dimensional Scaffolds Biocompatibility
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.6/4661 |
Resumo: | While developing tissue engineering strategies, inflammatory response caused by biomaterials is an unavoidable aspect to be taken into consideration, as it may be an early limiting step of tissue regeneration approaches. We demonstrate the application of flat and flexible films exhibiting patterned high-contrast wettability regions as implantable platforms for the high-content in vivo study of inflammatory response caused by biomaterials. Screening biomaterials by using high-throughput platforms is a powerful method to detect hit spots with promising properties and to exclude uninteresting conditions for targeted applications. High-content analysis of biomaterials has been mostly restricted to in vitro tests where crucial information is lost, as in vivo environment is highly complex. Conventional biomaterials implantation requires the use of high numbers of animals, leading to ethical questions and costly experimentation. Inflammatory response of biomaterials has also been highly neglected in high-throughput studies. We designed an array of 36 combinations of biomaterials based on an initial library of four polysaccharides. Biomaterials were dispensed onto biomimetic superhydrophobic platforms with wettable regions and processed as freeze-dried three-dimensional scaffolds with a high control of the array configuration. These chips were afterward implanted subcutaneously in Wistar rats. Lymphocyte recruitment and activated macrophages were studied on-chip, by performing immunocytochemistry in the miniaturized biomaterials after 24 h and 7 days of implantation. Histological cuts of the surrounding tissue of the implants were also analyzed. Localized and independent inflammatory responses were detected. The integration of these data with control data proved that these chips are robust platforms for the rapid screening of early-stage in vivo biomaterials' response. |
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In Vivo High-Content Evaluation of Three-Dimensional Scaffolds BiocompatibilityScaffoldsBiocompatibilityThree DimensionalWhile developing tissue engineering strategies, inflammatory response caused by biomaterials is an unavoidable aspect to be taken into consideration, as it may be an early limiting step of tissue regeneration approaches. We demonstrate the application of flat and flexible films exhibiting patterned high-contrast wettability regions as implantable platforms for the high-content in vivo study of inflammatory response caused by biomaterials. Screening biomaterials by using high-throughput platforms is a powerful method to detect hit spots with promising properties and to exclude uninteresting conditions for targeted applications. High-content analysis of biomaterials has been mostly restricted to in vitro tests where crucial information is lost, as in vivo environment is highly complex. Conventional biomaterials implantation requires the use of high numbers of animals, leading to ethical questions and costly experimentation. Inflammatory response of biomaterials has also been highly neglected in high-throughput studies. We designed an array of 36 combinations of biomaterials based on an initial library of four polysaccharides. Biomaterials were dispensed onto biomimetic superhydrophobic platforms with wettable regions and processed as freeze-dried three-dimensional scaffolds with a high control of the array configuration. These chips were afterward implanted subcutaneously in Wistar rats. Lymphocyte recruitment and activated macrophages were studied on-chip, by performing immunocytochemistry in the miniaturized biomaterials after 24 h and 7 days of implantation. Histological cuts of the surrounding tissue of the implants were also analyzed. Localized and independent inflammatory responses were detected. The integration of these data with control data proved that these chips are robust platforms for the rapid screening of early-stage in vivo biomaterials' response.M.B.O. acknowledges Fundação para a Ciência e para a Tecnologia for the PhD grant SFRH/BD/71396/2010. The research leading to these results has received funding from the European Union’s Seventh Framework Programme (FP7/ 2007-2013) under grant agreement no. REGPOT-CT2012- 316331-POLARIS. The research was also funded by FEDER through the Competitive Factors Operation Program— COMPETE and by National funds through FCT—Fundação para a Ciência e a Tecnologia in the scope of the projects PTDC/CTM-BIO/1814/2012, Pest-C/SAU/UI0709/2011 and PEst-OE/EGE/UI4056/2011.Mary Ann LiebertuBibliorumOliveira, MarianaRibeiro, MP.Miguel, SóniaNeto, AnaCoutinho, Paula Isabel Teixeira GonçalvesCorreia, Ilídio Joaquim SobreiraMano, João F.2018-03-20T14:53:03Z2014-02-252014-02-25T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.6/4661engOliveira, M.B., Ribeiro, M.P., Miguel, S.P., Neto, A.I., Coutinho, P., Correia, I.J. e Mano, J.F. (2014) “In vivo high-content evaluation of three-dimensional scaffolds biocompatibility”, Tissue Engineering, Vol. 20(11): pp. 851-86410.1089/ten.TEC.2013.0738metadata only accessinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-11-27T12:15:03Zoai:ubibliorum.ubi.pt:10400.6/4661Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-11-27T12:15:03Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
In Vivo High-Content Evaluation of Three-Dimensional Scaffolds Biocompatibility |
title |
In Vivo High-Content Evaluation of Three-Dimensional Scaffolds Biocompatibility |
spellingShingle |
In Vivo High-Content Evaluation of Three-Dimensional Scaffolds Biocompatibility Oliveira, Mariana Scaffolds Biocompatibility Three Dimensional |
title_short |
In Vivo High-Content Evaluation of Three-Dimensional Scaffolds Biocompatibility |
title_full |
In Vivo High-Content Evaluation of Three-Dimensional Scaffolds Biocompatibility |
title_fullStr |
In Vivo High-Content Evaluation of Three-Dimensional Scaffolds Biocompatibility |
title_full_unstemmed |
In Vivo High-Content Evaluation of Three-Dimensional Scaffolds Biocompatibility |
title_sort |
In Vivo High-Content Evaluation of Three-Dimensional Scaffolds Biocompatibility |
author |
Oliveira, Mariana |
author_facet |
Oliveira, Mariana Ribeiro, MP. Miguel, Sónia Neto, Ana Coutinho, Paula Isabel Teixeira Gonçalves Correia, Ilídio Joaquim Sobreira Mano, João F. |
author_role |
author |
author2 |
Ribeiro, MP. Miguel, Sónia Neto, Ana Coutinho, Paula Isabel Teixeira Gonçalves Correia, Ilídio Joaquim Sobreira Mano, João F. |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
uBibliorum |
dc.contributor.author.fl_str_mv |
Oliveira, Mariana Ribeiro, MP. Miguel, Sónia Neto, Ana Coutinho, Paula Isabel Teixeira Gonçalves Correia, Ilídio Joaquim Sobreira Mano, João F. |
dc.subject.por.fl_str_mv |
Scaffolds Biocompatibility Three Dimensional |
topic |
Scaffolds Biocompatibility Three Dimensional |
description |
While developing tissue engineering strategies, inflammatory response caused by biomaterials is an unavoidable aspect to be taken into consideration, as it may be an early limiting step of tissue regeneration approaches. We demonstrate the application of flat and flexible films exhibiting patterned high-contrast wettability regions as implantable platforms for the high-content in vivo study of inflammatory response caused by biomaterials. Screening biomaterials by using high-throughput platforms is a powerful method to detect hit spots with promising properties and to exclude uninteresting conditions for targeted applications. High-content analysis of biomaterials has been mostly restricted to in vitro tests where crucial information is lost, as in vivo environment is highly complex. Conventional biomaterials implantation requires the use of high numbers of animals, leading to ethical questions and costly experimentation. Inflammatory response of biomaterials has also been highly neglected in high-throughput studies. We designed an array of 36 combinations of biomaterials based on an initial library of four polysaccharides. Biomaterials were dispensed onto biomimetic superhydrophobic platforms with wettable regions and processed as freeze-dried three-dimensional scaffolds with a high control of the array configuration. These chips were afterward implanted subcutaneously in Wistar rats. Lymphocyte recruitment and activated macrophages were studied on-chip, by performing immunocytochemistry in the miniaturized biomaterials after 24 h and 7 days of implantation. Histological cuts of the surrounding tissue of the implants were also analyzed. Localized and independent inflammatory responses were detected. The integration of these data with control data proved that these chips are robust platforms for the rapid screening of early-stage in vivo biomaterials' response. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-02-25 2014-02-25T00:00:00Z 2018-03-20T14:53:03Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.6/4661 |
url |
http://hdl.handle.net/10400.6/4661 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Oliveira, M.B., Ribeiro, M.P., Miguel, S.P., Neto, A.I., Coutinho, P., Correia, I.J. e Mano, J.F. (2014) “In vivo high-content evaluation of three-dimensional scaffolds biocompatibility”, Tissue Engineering, Vol. 20(11): pp. 851-864 10.1089/ten.TEC.2013.0738 |
dc.rights.driver.fl_str_mv |
metadata only access info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
metadata only access |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Mary Ann Liebert |
publisher.none.fl_str_mv |
Mary Ann Liebert |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
mluisa.alvim@gmail.com |
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1817549604272472064 |