In Vivo High-Content Evaluation of Three-Dimensional Scaffolds Biocompatibility

Detalhes bibliográficos
Autor(a) principal: Oliveira, Mariana
Data de Publicação: 2014
Outros Autores: Ribeiro, MP., Miguel, Sónia, Neto, Ana, Coutinho, Paula Isabel Teixeira Gonçalves, Correia, Ilídio Joaquim Sobreira, Mano, João F.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.6/4661
Resumo: While developing tissue engineering strategies, inflammatory response caused by biomaterials is an unavoidable aspect to be taken into consideration, as it may be an early limiting step of tissue regeneration approaches. We demonstrate the application of flat and flexible films exhibiting patterned high-contrast wettability regions as implantable platforms for the high-content in vivo study of inflammatory response caused by biomaterials. Screening biomaterials by using high-throughput platforms is a powerful method to detect hit spots with promising properties and to exclude uninteresting conditions for targeted applications. High-content analysis of biomaterials has been mostly restricted to in vitro tests where crucial information is lost, as in vivo environment is highly complex. Conventional biomaterials implantation requires the use of high numbers of animals, leading to ethical questions and costly experimentation. Inflammatory response of biomaterials has also been highly neglected in high-throughput studies. We designed an array of 36 combinations of biomaterials based on an initial library of four polysaccharides. Biomaterials were dispensed onto biomimetic superhydrophobic platforms with wettable regions and processed as freeze-dried three-dimensional scaffolds with a high control of the array configuration. These chips were afterward implanted subcutaneously in Wistar rats. Lymphocyte recruitment and activated macrophages were studied on-chip, by performing immunocytochemistry in the miniaturized biomaterials after 24 h and 7 days of implantation. Histological cuts of the surrounding tissue of the implants were also analyzed. Localized and independent inflammatory responses were detected. The integration of these data with control data proved that these chips are robust platforms for the rapid screening of early-stage in vivo biomaterials' response.
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spelling In Vivo High-Content Evaluation of Three-Dimensional Scaffolds BiocompatibilityScaffoldsBiocompatibilityThree DimensionalWhile developing tissue engineering strategies, inflammatory response caused by biomaterials is an unavoidable aspect to be taken into consideration, as it may be an early limiting step of tissue regeneration approaches. We demonstrate the application of flat and flexible films exhibiting patterned high-contrast wettability regions as implantable platforms for the high-content in vivo study of inflammatory response caused by biomaterials. Screening biomaterials by using high-throughput platforms is a powerful method to detect hit spots with promising properties and to exclude uninteresting conditions for targeted applications. High-content analysis of biomaterials has been mostly restricted to in vitro tests where crucial information is lost, as in vivo environment is highly complex. Conventional biomaterials implantation requires the use of high numbers of animals, leading to ethical questions and costly experimentation. Inflammatory response of biomaterials has also been highly neglected in high-throughput studies. We designed an array of 36 combinations of biomaterials based on an initial library of four polysaccharides. Biomaterials were dispensed onto biomimetic superhydrophobic platforms with wettable regions and processed as freeze-dried three-dimensional scaffolds with a high control of the array configuration. These chips were afterward implanted subcutaneously in Wistar rats. Lymphocyte recruitment and activated macrophages were studied on-chip, by performing immunocytochemistry in the miniaturized biomaterials after 24 h and 7 days of implantation. Histological cuts of the surrounding tissue of the implants were also analyzed. Localized and independent inflammatory responses were detected. The integration of these data with control data proved that these chips are robust platforms for the rapid screening of early-stage in vivo biomaterials' response.M.B.O. acknowledges Fundação para a Ciência e para a Tecnologia for the PhD grant SFRH/BD/71396/2010. The research leading to these results has received funding from the European Union’s Seventh Framework Programme (FP7/ 2007-2013) under grant agreement no. REGPOT-CT2012- 316331-POLARIS. The research was also funded by FEDER through the Competitive Factors Operation Program— COMPETE and by National funds through FCT—Fundação para a Ciência e a Tecnologia in the scope of the projects PTDC/CTM-BIO/1814/2012, Pest-C/SAU/UI0709/2011 and PEst-OE/EGE/UI4056/2011.Mary Ann LiebertuBibliorumOliveira, MarianaRibeiro, MP.Miguel, SóniaNeto, AnaCoutinho, Paula Isabel Teixeira GonçalvesCorreia, Ilídio Joaquim SobreiraMano, João F.2018-03-20T14:53:03Z2014-02-252014-02-25T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.6/4661engOliveira, M.B., Ribeiro, M.P., Miguel, S.P., Neto, A.I., Coutinho, P., Correia, I.J. e Mano, J.F. (2014) “In vivo high-content evaluation of three-dimensional scaffolds biocompatibility”, Tissue Engineering, Vol. 20(11): pp. 851-86410.1089/ten.TEC.2013.0738metadata only accessinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-11-27T12:15:03Zoai:ubibliorum.ubi.pt:10400.6/4661Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-11-27T12:15:03Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv In Vivo High-Content Evaluation of Three-Dimensional Scaffolds Biocompatibility
title In Vivo High-Content Evaluation of Three-Dimensional Scaffolds Biocompatibility
spellingShingle In Vivo High-Content Evaluation of Three-Dimensional Scaffolds Biocompatibility
Oliveira, Mariana
Scaffolds
Biocompatibility
Three Dimensional
title_short In Vivo High-Content Evaluation of Three-Dimensional Scaffolds Biocompatibility
title_full In Vivo High-Content Evaluation of Three-Dimensional Scaffolds Biocompatibility
title_fullStr In Vivo High-Content Evaluation of Three-Dimensional Scaffolds Biocompatibility
title_full_unstemmed In Vivo High-Content Evaluation of Three-Dimensional Scaffolds Biocompatibility
title_sort In Vivo High-Content Evaluation of Three-Dimensional Scaffolds Biocompatibility
author Oliveira, Mariana
author_facet Oliveira, Mariana
Ribeiro, MP.
Miguel, Sónia
Neto, Ana
Coutinho, Paula Isabel Teixeira Gonçalves
Correia, Ilídio Joaquim Sobreira
Mano, João F.
author_role author
author2 Ribeiro, MP.
Miguel, Sónia
Neto, Ana
Coutinho, Paula Isabel Teixeira Gonçalves
Correia, Ilídio Joaquim Sobreira
Mano, João F.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv uBibliorum
dc.contributor.author.fl_str_mv Oliveira, Mariana
Ribeiro, MP.
Miguel, Sónia
Neto, Ana
Coutinho, Paula Isabel Teixeira Gonçalves
Correia, Ilídio Joaquim Sobreira
Mano, João F.
dc.subject.por.fl_str_mv Scaffolds
Biocompatibility
Three Dimensional
topic Scaffolds
Biocompatibility
Three Dimensional
description While developing tissue engineering strategies, inflammatory response caused by biomaterials is an unavoidable aspect to be taken into consideration, as it may be an early limiting step of tissue regeneration approaches. We demonstrate the application of flat and flexible films exhibiting patterned high-contrast wettability regions as implantable platforms for the high-content in vivo study of inflammatory response caused by biomaterials. Screening biomaterials by using high-throughput platforms is a powerful method to detect hit spots with promising properties and to exclude uninteresting conditions for targeted applications. High-content analysis of biomaterials has been mostly restricted to in vitro tests where crucial information is lost, as in vivo environment is highly complex. Conventional biomaterials implantation requires the use of high numbers of animals, leading to ethical questions and costly experimentation. Inflammatory response of biomaterials has also been highly neglected in high-throughput studies. We designed an array of 36 combinations of biomaterials based on an initial library of four polysaccharides. Biomaterials were dispensed onto biomimetic superhydrophobic platforms with wettable regions and processed as freeze-dried three-dimensional scaffolds with a high control of the array configuration. These chips were afterward implanted subcutaneously in Wistar rats. Lymphocyte recruitment and activated macrophages were studied on-chip, by performing immunocytochemistry in the miniaturized biomaterials after 24 h and 7 days of implantation. Histological cuts of the surrounding tissue of the implants were also analyzed. Localized and independent inflammatory responses were detected. The integration of these data with control data proved that these chips are robust platforms for the rapid screening of early-stage in vivo biomaterials' response.
publishDate 2014
dc.date.none.fl_str_mv 2014-02-25
2014-02-25T00:00:00Z
2018-03-20T14:53:03Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.6/4661
url http://hdl.handle.net/10400.6/4661
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Oliveira, M.B., Ribeiro, M.P., Miguel, S.P., Neto, A.I., Coutinho, P., Correia, I.J. e Mano, J.F. (2014) “In vivo high-content evaluation of three-dimensional scaffolds biocompatibility”, Tissue Engineering, Vol. 20(11): pp. 851-864
10.1089/ten.TEC.2013.0738
dc.rights.driver.fl_str_mv metadata only access
info:eu-repo/semantics/openAccess
rights_invalid_str_mv metadata only access
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Mary Ann Liebert
publisher.none.fl_str_mv Mary Ann Liebert
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv mluisa.alvim@gmail.com
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