Heat and water stress induce unique transcriptional signatures of heat-shock proteins and transcription factors in grapevine
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.7/715 |
Resumo: | Grapevine is an extremely important crop worldwide.In southern Europe, post-flowering phases of the growth cycle can occur under high temperatures, excessive light, and drought conditions at soil and/or atmospheric level. In this study, we subjected greenhouse grown grapevine, variety Aragonez, to two individual abiotic stresses, water deficit stress(WDS), and heat stress (HS). The adaptation of plants to stress is a complex response triggered by cascades of molecular net works involved in stress perception, signal transduction, and the expression of specific stress-related genes and metabolites. Approaches such as array-based transcript profiling allow assessing the expression of thousands of genes in control and stress tissues. Using microarrays, we analyzed the leaf transcriptomic profile of the grapevine plants. Photosynthesis measurements verified that the plants were significantly affected by the stresses applied. Leaf gene expression was obtained using a high-throughput transcriptomic grapevine array, the 23K custom-made Affymetrix Vitis GeneChip. We identified 1,594 genes as differentially expressed between control and treatments and grouped them into ten major functional categories using MapMan software. The transcriptome of Aragonez was more significantly affected by HS when compared with WDS. The number of genes coding for heat-shock proteins and transcription factors expressed solely in response to HS suggesting their expression as unique signatures of HS. However, across-talk between the response pathways to both stresses was observed at the level of AP2/ERF transcription factors. |
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Heat and water stress induce unique transcriptional signatures of heat-shock proteins and transcription factors in grapevineCluster AnalysisDehydrationHeat-Shock ProteinsOligonucleotide Array Sequence AnalysisPlant LeavesPlant ProteinsStress, PhysiologicalTranscription FactorsTranscriptomeVitisGene Expression Regulation, PlantGrapevine is an extremely important crop worldwide.In southern Europe, post-flowering phases of the growth cycle can occur under high temperatures, excessive light, and drought conditions at soil and/or atmospheric level. In this study, we subjected greenhouse grown grapevine, variety Aragonez, to two individual abiotic stresses, water deficit stress(WDS), and heat stress (HS). The adaptation of plants to stress is a complex response triggered by cascades of molecular net works involved in stress perception, signal transduction, and the expression of specific stress-related genes and metabolites. Approaches such as array-based transcript profiling allow assessing the expression of thousands of genes in control and stress tissues. Using microarrays, we analyzed the leaf transcriptomic profile of the grapevine plants. Photosynthesis measurements verified that the plants were significantly affected by the stresses applied. Leaf gene expression was obtained using a high-throughput transcriptomic grapevine array, the 23K custom-made Affymetrix Vitis GeneChip. We identified 1,594 genes as differentially expressed between control and treatments and grouped them into ten major functional categories using MapMan software. The transcriptome of Aragonez was more significantly affected by HS when compared with WDS. The number of genes coding for heat-shock proteins and transcription factors expressed solely in response to HS suggesting their expression as unique signatures of HS. However, across-talk between the response pathways to both stresses was observed at the level of AP2/ERF transcription factors.ERA-NET Plant Genomics 006/2006.Springer VerlagARCARocheta, MargaridaBecker, Jörg D.Coito, João L.Carvalho, LuísaAmâncio, Sara2016-11-16T18:24:44Z2013-10-122013-10-12T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfapplication/vnd.openxmlformats-officedocument.wordprocessingml.documentapplication/vnd.openxmlformats-officedocument.wordprocessingml.documentapplication/vnd.openxmlformats-officedocument.spreadsheetml.sheetapplication/vnd.openxmlformats-officedocument.spreadsheetml.sheetapplication/pdfapplication/vnd.openxmlformats-officedocument.presentationml.presentationapplication/vnd.openxmlformats-officedocument.spreadsheetml.sheethttp://hdl.handle.net/10400.7/715engRocheta, M., Becker, J.D., Coito, J.L. et al. Funct Integr Genomics (2014) 14: 135. doi:10.1007/s10142-013-0338-z10.1007/s10142-013-0338-zinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-11-29T14:35:06Zoai:arca.igc.gulbenkian.pt:10400.7/715Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T16:11:56.874421Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Heat and water stress induce unique transcriptional signatures of heat-shock proteins and transcription factors in grapevine |
title |
Heat and water stress induce unique transcriptional signatures of heat-shock proteins and transcription factors in grapevine |
spellingShingle |
Heat and water stress induce unique transcriptional signatures of heat-shock proteins and transcription factors in grapevine Rocheta, Margarida Cluster Analysis Dehydration Heat-Shock Proteins Oligonucleotide Array Sequence Analysis Plant Leaves Plant Proteins Stress, Physiological Transcription Factors Transcriptome Vitis Gene Expression Regulation, Plant |
title_short |
Heat and water stress induce unique transcriptional signatures of heat-shock proteins and transcription factors in grapevine |
title_full |
Heat and water stress induce unique transcriptional signatures of heat-shock proteins and transcription factors in grapevine |
title_fullStr |
Heat and water stress induce unique transcriptional signatures of heat-shock proteins and transcription factors in grapevine |
title_full_unstemmed |
Heat and water stress induce unique transcriptional signatures of heat-shock proteins and transcription factors in grapevine |
title_sort |
Heat and water stress induce unique transcriptional signatures of heat-shock proteins and transcription factors in grapevine |
author |
Rocheta, Margarida |
author_facet |
Rocheta, Margarida Becker, Jörg D. Coito, João L. Carvalho, Luísa Amâncio, Sara |
author_role |
author |
author2 |
Becker, Jörg D. Coito, João L. Carvalho, Luísa Amâncio, Sara |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
ARCA |
dc.contributor.author.fl_str_mv |
Rocheta, Margarida Becker, Jörg D. Coito, João L. Carvalho, Luísa Amâncio, Sara |
dc.subject.por.fl_str_mv |
Cluster Analysis Dehydration Heat-Shock Proteins Oligonucleotide Array Sequence Analysis Plant Leaves Plant Proteins Stress, Physiological Transcription Factors Transcriptome Vitis Gene Expression Regulation, Plant |
topic |
Cluster Analysis Dehydration Heat-Shock Proteins Oligonucleotide Array Sequence Analysis Plant Leaves Plant Proteins Stress, Physiological Transcription Factors Transcriptome Vitis Gene Expression Regulation, Plant |
description |
Grapevine is an extremely important crop worldwide.In southern Europe, post-flowering phases of the growth cycle can occur under high temperatures, excessive light, and drought conditions at soil and/or atmospheric level. In this study, we subjected greenhouse grown grapevine, variety Aragonez, to two individual abiotic stresses, water deficit stress(WDS), and heat stress (HS). The adaptation of plants to stress is a complex response triggered by cascades of molecular net works involved in stress perception, signal transduction, and the expression of specific stress-related genes and metabolites. Approaches such as array-based transcript profiling allow assessing the expression of thousands of genes in control and stress tissues. Using microarrays, we analyzed the leaf transcriptomic profile of the grapevine plants. Photosynthesis measurements verified that the plants were significantly affected by the stresses applied. Leaf gene expression was obtained using a high-throughput transcriptomic grapevine array, the 23K custom-made Affymetrix Vitis GeneChip. We identified 1,594 genes as differentially expressed between control and treatments and grouped them into ten major functional categories using MapMan software. The transcriptome of Aragonez was more significantly affected by HS when compared with WDS. The number of genes coding for heat-shock proteins and transcription factors expressed solely in response to HS suggesting their expression as unique signatures of HS. However, across-talk between the response pathways to both stresses was observed at the level of AP2/ERF transcription factors. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-10-12 2013-10-12T00:00:00Z 2016-11-16T18:24:44Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.7/715 |
url |
http://hdl.handle.net/10400.7/715 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Rocheta, M., Becker, J.D., Coito, J.L. et al. Funct Integr Genomics (2014) 14: 135. doi:10.1007/s10142-013-0338-z 10.1007/s10142-013-0338-z |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf application/vnd.openxmlformats-officedocument.wordprocessingml.document application/vnd.openxmlformats-officedocument.wordprocessingml.document application/vnd.openxmlformats-officedocument.spreadsheetml.sheet application/vnd.openxmlformats-officedocument.spreadsheetml.sheet application/pdf application/vnd.openxmlformats-officedocument.presentationml.presentation application/vnd.openxmlformats-officedocument.spreadsheetml.sheet |
dc.publisher.none.fl_str_mv |
Springer Verlag |
publisher.none.fl_str_mv |
Springer Verlag |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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