Red wine-dependent reduction of nitrite to nitric oxide in the stomach
Autor(a) principal: | |
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Data de Publicação: | 2007 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10316/5851 https://doi.org/10.1016/j.freeradbiomed.2007.07.018 |
Resumo: | Nitrite may be a source for nitric oxide (NO), particularly in highly acidic environments, such as the stomach. Diet products contribute also with reductants that dramatically increase the production of NO from nitrite. Red wine has been attributed health promoting properties largely on basis of the reductive antioxidant properties of its polyphenolic fraction. We show in vitro that wine, wine anthocyanin fraction and wine catechol (caffeic acid) dose- and pH-dependently promote the formation of NO when mixed with nitrite, as measured electrochemically. The production of NO promoted by wine from nitrite was substantiated in vivo in healthy volunteers by measuring NO in the air expelled from the stomach, following consumption of wine, as measured by chemiluminescence. Mechanistically, the reaction involves the univalent reduction of nitrite, as suggested by the formation of NO and by the appearance of EPR spectra assigned to wine phenolic radicals. Ascorbic and caffeic acids cooperate in the reduction of nitrite to NO. Moreover, reduction of nitrite is critically dependent on the phenolic structure and nitro-derivatives of phenols are also formed, as suggested by caffeic acid UV spectral modifications. The reduction of nitrite may reveal previously unrecognized physiologic effects of red wine in connection with NO bioactivity. |
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Red wine-dependent reduction of nitrite to nitric oxide in the stomachRed wineNitriteNitric oxidePhenolic compoundsStomachFree radicalsNitrite may be a source for nitric oxide (NO), particularly in highly acidic environments, such as the stomach. Diet products contribute also with reductants that dramatically increase the production of NO from nitrite. Red wine has been attributed health promoting properties largely on basis of the reductive antioxidant properties of its polyphenolic fraction. We show in vitro that wine, wine anthocyanin fraction and wine catechol (caffeic acid) dose- and pH-dependently promote the formation of NO when mixed with nitrite, as measured electrochemically. The production of NO promoted by wine from nitrite was substantiated in vivo in healthy volunteers by measuring NO in the air expelled from the stomach, following consumption of wine, as measured by chemiluminescence. Mechanistically, the reaction involves the univalent reduction of nitrite, as suggested by the formation of NO and by the appearance of EPR spectra assigned to wine phenolic radicals. Ascorbic and caffeic acids cooperate in the reduction of nitrite to NO. Moreover, reduction of nitrite is critically dependent on the phenolic structure and nitro-derivatives of phenols are also formed, as suggested by caffeic acid UV spectral modifications. The reduction of nitrite may reveal previously unrecognized physiologic effects of red wine in connection with NO bioactivity.http://www.sciencedirect.com/science/article/B6T38-4NYJ0RC-7/1/b6fd966c94e8278f5878bdf9252566e62007info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleaplication/PDFhttp://hdl.handle.net/10316/5851http://hdl.handle.net/10316/5851https://doi.org/10.1016/j.freeradbiomed.2007.07.018engFree Radical Biology and Medicine. 43:9 (2007) 1233-1242Gago, BrunoLundberg, Jon O.Barbosa, Rui M.Laranjinha, Joãoinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2021-08-31T07:46:52Zoai:estudogeral.uc.pt:10316/5851Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:47:19.673793Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Red wine-dependent reduction of nitrite to nitric oxide in the stomach |
title |
Red wine-dependent reduction of nitrite to nitric oxide in the stomach |
spellingShingle |
Red wine-dependent reduction of nitrite to nitric oxide in the stomach Gago, Bruno Red wine Nitrite Nitric oxide Phenolic compounds Stomach Free radicals |
title_short |
Red wine-dependent reduction of nitrite to nitric oxide in the stomach |
title_full |
Red wine-dependent reduction of nitrite to nitric oxide in the stomach |
title_fullStr |
Red wine-dependent reduction of nitrite to nitric oxide in the stomach |
title_full_unstemmed |
Red wine-dependent reduction of nitrite to nitric oxide in the stomach |
title_sort |
Red wine-dependent reduction of nitrite to nitric oxide in the stomach |
author |
Gago, Bruno |
author_facet |
Gago, Bruno Lundberg, Jon O. Barbosa, Rui M. Laranjinha, João |
author_role |
author |
author2 |
Lundberg, Jon O. Barbosa, Rui M. Laranjinha, João |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Gago, Bruno Lundberg, Jon O. Barbosa, Rui M. Laranjinha, João |
dc.subject.por.fl_str_mv |
Red wine Nitrite Nitric oxide Phenolic compounds Stomach Free radicals |
topic |
Red wine Nitrite Nitric oxide Phenolic compounds Stomach Free radicals |
description |
Nitrite may be a source for nitric oxide (NO), particularly in highly acidic environments, such as the stomach. Diet products contribute also with reductants that dramatically increase the production of NO from nitrite. Red wine has been attributed health promoting properties largely on basis of the reductive antioxidant properties of its polyphenolic fraction. We show in vitro that wine, wine anthocyanin fraction and wine catechol (caffeic acid) dose- and pH-dependently promote the formation of NO when mixed with nitrite, as measured electrochemically. The production of NO promoted by wine from nitrite was substantiated in vivo in healthy volunteers by measuring NO in the air expelled from the stomach, following consumption of wine, as measured by chemiluminescence. Mechanistically, the reaction involves the univalent reduction of nitrite, as suggested by the formation of NO and by the appearance of EPR spectra assigned to wine phenolic radicals. Ascorbic and caffeic acids cooperate in the reduction of nitrite to NO. Moreover, reduction of nitrite is critically dependent on the phenolic structure and nitro-derivatives of phenols are also formed, as suggested by caffeic acid UV spectral modifications. The reduction of nitrite may reveal previously unrecognized physiologic effects of red wine in connection with NO bioactivity. |
publishDate |
2007 |
dc.date.none.fl_str_mv |
2007 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10316/5851 http://hdl.handle.net/10316/5851 https://doi.org/10.1016/j.freeradbiomed.2007.07.018 |
url |
http://hdl.handle.net/10316/5851 https://doi.org/10.1016/j.freeradbiomed.2007.07.018 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Free Radical Biology and Medicine. 43:9 (2007) 1233-1242 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
aplication/PDF |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799133750582312960 |