The potent vasodilator ethyl nitrite is formed upon reaction of nitrite and ethanol under gastric conditions
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10316/5829 https://doi.org/10.1016/j.freeradbiomed.2008.04.027 |
Resumo: | By acting as a bioreactor, affording chemical and mechanical conditions for the reaction between dietary components, the stomach may be a source of new bioactive molecules. Using gas chromatography-mass spectrometry we here demonstrate that, under acidic gastric conditions, ethyl nitrite is formed in µM concentrations from the reaction of red wine or distilled alcoholic drinks with physiological amounts of nitrite. Rat femoral artery rings and gastric fundus strips dose-dependently relaxed upon exposure to nitrite:ethanol mixtures. In contrast, when administered separately in the same dose ranges, nitrite evoked only minor vasorelaxation while ethanol actually caused a slight vasoconstriction. Mechanistically, the relaxation effect was assigned to generation of nitric oxide (-NO) as supported by direct demonstration of -NO release from ethyl nitrite and the absence of relaxation in the presence of the soluble guanylyl cyclase inhibitor, ODQ. In conclusion, these results suggest that ethanol in alcoholic drinks interacts with salivary-derived nitrite in the acidic stomach leading to the production of the potent smooth muscle relaxant ethyl nitrite. These findings reveal an alternative chemical reaction pathway for dietary nitrate and nitrite with possible impact on gastric physiology and pathophysiology. |
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The potent vasodilator ethyl nitrite is formed upon reaction of nitrite and ethanol under gastric conditionsEthyl nitriteNitriteEthanolRed wineVasodilatationStomachBy acting as a bioreactor, affording chemical and mechanical conditions for the reaction between dietary components, the stomach may be a source of new bioactive molecules. Using gas chromatography-mass spectrometry we here demonstrate that, under acidic gastric conditions, ethyl nitrite is formed in µM concentrations from the reaction of red wine or distilled alcoholic drinks with physiological amounts of nitrite. Rat femoral artery rings and gastric fundus strips dose-dependently relaxed upon exposure to nitrite:ethanol mixtures. In contrast, when administered separately in the same dose ranges, nitrite evoked only minor vasorelaxation while ethanol actually caused a slight vasoconstriction. Mechanistically, the relaxation effect was assigned to generation of nitric oxide (-NO) as supported by direct demonstration of -NO release from ethyl nitrite and the absence of relaxation in the presence of the soluble guanylyl cyclase inhibitor, ODQ. In conclusion, these results suggest that ethanol in alcoholic drinks interacts with salivary-derived nitrite in the acidic stomach leading to the production of the potent smooth muscle relaxant ethyl nitrite. These findings reveal an alternative chemical reaction pathway for dietary nitrate and nitrite with possible impact on gastric physiology and pathophysiology.http://www.sciencedirect.com/science/article/B6T38-4SCDB0R-4/1/5f2ae4cd1ef8af7eca3f5d4ee8f353ad2008info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleaplication/PDFhttp://hdl.handle.net/10316/5829http://hdl.handle.net/10316/5829https://doi.org/10.1016/j.freeradbiomed.2008.04.027engFree Radical Biology and Medicine. 45:4 (2008) 404-412Gago, BrunoNyström, ThomasCavaleiro, CarlosRocha, Bárbara S.Barbosa, Rui M.Laranjinha, JoãoLundberg, Jon O.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2021-10-19T08:08:48Zoai:estudogeral.uc.pt:10316/5829Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:47:19.458797Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
The potent vasodilator ethyl nitrite is formed upon reaction of nitrite and ethanol under gastric conditions |
title |
The potent vasodilator ethyl nitrite is formed upon reaction of nitrite and ethanol under gastric conditions |
spellingShingle |
The potent vasodilator ethyl nitrite is formed upon reaction of nitrite and ethanol under gastric conditions Gago, Bruno Ethyl nitrite Nitrite Ethanol Red wine Vasodilatation Stomach |
title_short |
The potent vasodilator ethyl nitrite is formed upon reaction of nitrite and ethanol under gastric conditions |
title_full |
The potent vasodilator ethyl nitrite is formed upon reaction of nitrite and ethanol under gastric conditions |
title_fullStr |
The potent vasodilator ethyl nitrite is formed upon reaction of nitrite and ethanol under gastric conditions |
title_full_unstemmed |
The potent vasodilator ethyl nitrite is formed upon reaction of nitrite and ethanol under gastric conditions |
title_sort |
The potent vasodilator ethyl nitrite is formed upon reaction of nitrite and ethanol under gastric conditions |
author |
Gago, Bruno |
author_facet |
Gago, Bruno Nyström, Thomas Cavaleiro, Carlos Rocha, Bárbara S. Barbosa, Rui M. Laranjinha, João Lundberg, Jon O. |
author_role |
author |
author2 |
Nyström, Thomas Cavaleiro, Carlos Rocha, Bárbara S. Barbosa, Rui M. Laranjinha, João Lundberg, Jon O. |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Gago, Bruno Nyström, Thomas Cavaleiro, Carlos Rocha, Bárbara S. Barbosa, Rui M. Laranjinha, João Lundberg, Jon O. |
dc.subject.por.fl_str_mv |
Ethyl nitrite Nitrite Ethanol Red wine Vasodilatation Stomach |
topic |
Ethyl nitrite Nitrite Ethanol Red wine Vasodilatation Stomach |
description |
By acting as a bioreactor, affording chemical and mechanical conditions for the reaction between dietary components, the stomach may be a source of new bioactive molecules. Using gas chromatography-mass spectrometry we here demonstrate that, under acidic gastric conditions, ethyl nitrite is formed in µM concentrations from the reaction of red wine or distilled alcoholic drinks with physiological amounts of nitrite. Rat femoral artery rings and gastric fundus strips dose-dependently relaxed upon exposure to nitrite:ethanol mixtures. In contrast, when administered separately in the same dose ranges, nitrite evoked only minor vasorelaxation while ethanol actually caused a slight vasoconstriction. Mechanistically, the relaxation effect was assigned to generation of nitric oxide (-NO) as supported by direct demonstration of -NO release from ethyl nitrite and the absence of relaxation in the presence of the soluble guanylyl cyclase inhibitor, ODQ. In conclusion, these results suggest that ethanol in alcoholic drinks interacts with salivary-derived nitrite in the acidic stomach leading to the production of the potent smooth muscle relaxant ethyl nitrite. These findings reveal an alternative chemical reaction pathway for dietary nitrate and nitrite with possible impact on gastric physiology and pathophysiology. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10316/5829 http://hdl.handle.net/10316/5829 https://doi.org/10.1016/j.freeradbiomed.2008.04.027 |
url |
http://hdl.handle.net/10316/5829 https://doi.org/10.1016/j.freeradbiomed.2008.04.027 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Free Radical Biology and Medicine. 45:4 (2008) 404-412 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
aplication/PDF |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799133750576021504 |