Two probiotic candidates of the genus psychrobacter modulate the immune response and disease resistance after experimental infection in turbot (Scophthalmus maximus, Linnaeus 1758)
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.1/19478 |
Resumo: | Probiotic bacteria are a recognized alternative to classical methods of disease prophylaxis and therapy. We tested the effects of their application on the immune reaction in juvenile turbot. To prevent digestion of the probiotics, rectal administration was applied to maximise colonization, by-passing digestion in the stomach. The application of Psychrobacter nivimaris and Psychrobacter faecalis showed beneficial effects on the inflammatory response and disease resistance after infection with the common pathogen Tenacibaculum maritimum. Treatment with P. nivimaris and P. faecalis resulted in 0% and 8% mortalities post-infection, while in the treatment control, an elevated mortality of 20% was observed. In the challenge controls (no infection), no mortalities were observed during the entire experimental period. After an experimental infection, mRNA expression of selected immune markers (mhc II alpha, il-1 beta, tcr, tgf beta and tnf alpha) were determined by RT-QPCR at 0, 1 and 5 days post-infection (dpi). At 0 dpi, gene expression was comparable between the treatments and the treatment control, suggesting that probiotics did not act via immune stimulation of the host. At 1 dpi, all genes were up-regulated in the treatment control but not in the probiotic groups, indicating that the infection in probiotic-treated fish developed at a less severe level. At 5 dpi, mRNA expression returned to baseline levels. As a conclusion, the native probiotic candidates P. nivimaris and P. faecalis improved survival, whereas, in the control, mortality increased and expression of the immune markers was up-regulated post infection. This highlights a potential application of P. nivimaris and P. faecalis in disease prophylaxis, but further research is needed. |
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Two probiotic candidates of the genus psychrobacter modulate the immune response and disease resistance after experimental infection in turbot (Scophthalmus maximus, Linnaeus 1758)Native probioticsPsychrobacterPathogen challengeImmune markersSurvivalGene expressionProbiotic bacteria are a recognized alternative to classical methods of disease prophylaxis and therapy. We tested the effects of their application on the immune reaction in juvenile turbot. To prevent digestion of the probiotics, rectal administration was applied to maximise colonization, by-passing digestion in the stomach. The application of Psychrobacter nivimaris and Psychrobacter faecalis showed beneficial effects on the inflammatory response and disease resistance after infection with the common pathogen Tenacibaculum maritimum. Treatment with P. nivimaris and P. faecalis resulted in 0% and 8% mortalities post-infection, while in the treatment control, an elevated mortality of 20% was observed. In the challenge controls (no infection), no mortalities were observed during the entire experimental period. After an experimental infection, mRNA expression of selected immune markers (mhc II alpha, il-1 beta, tcr, tgf beta and tnf alpha) were determined by RT-QPCR at 0, 1 and 5 days post-infection (dpi). At 0 dpi, gene expression was comparable between the treatments and the treatment control, suggesting that probiotics did not act via immune stimulation of the host. At 1 dpi, all genes were up-regulated in the treatment control but not in the probiotic groups, indicating that the infection in probiotic-treated fish developed at a less severe level. At 5 dpi, mRNA expression returned to baseline levels. As a conclusion, the native probiotic candidates P. nivimaris and P. faecalis improved survival, whereas, in the control, mortality increased and expression of the immune markers was up-regulated post infection. This highlights a potential application of P. nivimaris and P. faecalis in disease prophylaxis, but further research is needed.FCT/DAAD Acções Integradas Luso-Alemãs (references441.00 DAAD and 57051461)MDPISapientiaWuertz, SvenBeça, FilipaKreuz, EvaWanka, Konrad M.Azeredo, RitaMachado, MarinaCostas, Benjamin2023-04-20T09:59:36Z2023-032023-03-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.1/19478eng10.3390/fishes80301442410-3888info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-24T10:31:57Zoai:sapientia.ualg.pt:10400.1/19478Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:09:06.584190Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Two probiotic candidates of the genus psychrobacter modulate the immune response and disease resistance after experimental infection in turbot (Scophthalmus maximus, Linnaeus 1758) |
title |
Two probiotic candidates of the genus psychrobacter modulate the immune response and disease resistance after experimental infection in turbot (Scophthalmus maximus, Linnaeus 1758) |
spellingShingle |
Two probiotic candidates of the genus psychrobacter modulate the immune response and disease resistance after experimental infection in turbot (Scophthalmus maximus, Linnaeus 1758) Wuertz, Sven Native probiotics Psychrobacter Pathogen challenge Immune markers Survival Gene expression |
title_short |
Two probiotic candidates of the genus psychrobacter modulate the immune response and disease resistance after experimental infection in turbot (Scophthalmus maximus, Linnaeus 1758) |
title_full |
Two probiotic candidates of the genus psychrobacter modulate the immune response and disease resistance after experimental infection in turbot (Scophthalmus maximus, Linnaeus 1758) |
title_fullStr |
Two probiotic candidates of the genus psychrobacter modulate the immune response and disease resistance after experimental infection in turbot (Scophthalmus maximus, Linnaeus 1758) |
title_full_unstemmed |
Two probiotic candidates of the genus psychrobacter modulate the immune response and disease resistance after experimental infection in turbot (Scophthalmus maximus, Linnaeus 1758) |
title_sort |
Two probiotic candidates of the genus psychrobacter modulate the immune response and disease resistance after experimental infection in turbot (Scophthalmus maximus, Linnaeus 1758) |
author |
Wuertz, Sven |
author_facet |
Wuertz, Sven Beça, Filipa Kreuz, Eva Wanka, Konrad M. Azeredo, Rita Machado, Marina Costas, Benjamin |
author_role |
author |
author2 |
Beça, Filipa Kreuz, Eva Wanka, Konrad M. Azeredo, Rita Machado, Marina Costas, Benjamin |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Sapientia |
dc.contributor.author.fl_str_mv |
Wuertz, Sven Beça, Filipa Kreuz, Eva Wanka, Konrad M. Azeredo, Rita Machado, Marina Costas, Benjamin |
dc.subject.por.fl_str_mv |
Native probiotics Psychrobacter Pathogen challenge Immune markers Survival Gene expression |
topic |
Native probiotics Psychrobacter Pathogen challenge Immune markers Survival Gene expression |
description |
Probiotic bacteria are a recognized alternative to classical methods of disease prophylaxis and therapy. We tested the effects of their application on the immune reaction in juvenile turbot. To prevent digestion of the probiotics, rectal administration was applied to maximise colonization, by-passing digestion in the stomach. The application of Psychrobacter nivimaris and Psychrobacter faecalis showed beneficial effects on the inflammatory response and disease resistance after infection with the common pathogen Tenacibaculum maritimum. Treatment with P. nivimaris and P. faecalis resulted in 0% and 8% mortalities post-infection, while in the treatment control, an elevated mortality of 20% was observed. In the challenge controls (no infection), no mortalities were observed during the entire experimental period. After an experimental infection, mRNA expression of selected immune markers (mhc II alpha, il-1 beta, tcr, tgf beta and tnf alpha) were determined by RT-QPCR at 0, 1 and 5 days post-infection (dpi). At 0 dpi, gene expression was comparable between the treatments and the treatment control, suggesting that probiotics did not act via immune stimulation of the host. At 1 dpi, all genes were up-regulated in the treatment control but not in the probiotic groups, indicating that the infection in probiotic-treated fish developed at a less severe level. At 5 dpi, mRNA expression returned to baseline levels. As a conclusion, the native probiotic candidates P. nivimaris and P. faecalis improved survival, whereas, in the control, mortality increased and expression of the immune markers was up-regulated post infection. This highlights a potential application of P. nivimaris and P. faecalis in disease prophylaxis, but further research is needed. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-04-20T09:59:36Z 2023-03 2023-03-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.1/19478 |
url |
http://hdl.handle.net/10400.1/19478 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.3390/fishes8030144 2410-3888 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
MDPI |
publisher.none.fl_str_mv |
MDPI |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799133338361921536 |