Genetic characterization of Portuguese Fasciola hepatica isolates

Detalhes bibliográficos
Autor(a) principal: Santos, Tânia Raquel Martins dos
Data de Publicação: 2012
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/8689
Resumo: Dissertation presented to obtain the Master Degree in Molecular, Genetics and Biomedicine
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spelling Genetic characterization of Portuguese Fasciola hepatica isolatesFasciola hepaticagenetic characterizationPortugalcox1nad1ITS regionDissertation presented to obtain the Master Degree in Molecular, Genetics and BiomedicinePart of the results discussed in this dissertation was presented in the following communications: R. Santos, M. Calado, J. Sampaio, C. Ferreira, A. Afonso and S. Belo. Contribution to the genetic characterization of Fasciola hepatica populations in Portugal. XXXVII Portuguese Genetic Conference, Lisbon, Portugal, May 28th-30th 2012 [poster communication] R. Santos, M. Calado, J. Sampaio, C. Ferreira, A. Afonso and S. Belo. Contribution to the genetic characterization of Fasciola hepatica populations in Portugal. Arquivos Portugueses das Ciências Biológicas. Tomo XXXVI (in press)Fasciola hepatica is a parasitic trematode with debilitating and socio-economically devastating effects. At present near to 600 million animals and 2.4 million people in the entire world suffer from fascioliasis. Genetic characterization is of the utmost importance to an efficient epidemiologic control of helminth infections. In the present study we aimed to provide the first insights into the genetic variability of F. hepatica in Portugal. 47 isolates from different hosts (cattle and sheep) and geographical locations (Beja, Castelo Branco, Coimbra, Évora, Faro, Leiria, Lisboa, Portalegre, Santarém and Setúbal) were analyzed through Random Amplified Polymorphic DNA-Polymerase Chain Reaction (RAPD-PCR), Restriction Fragment Length Polymorphism (RFLP) and sequencing of NADH dehydrogenase subunit 1 (nad1) gene, cytochrome c oxidase subunit 1 (cox1) gene and Internal Transcribed Spacers (ITS) region. RAPD-PCR and RFLP patterns were similar for all the analyzed samples, despite their host and geographical origin. Nucleotide sequencing revealed low levels of genetic diversity within Portuguese isolates and no direct correlation was observed between haplotype and geographical location or host. Phylogenetic analysis revealed a high similarity within samples from Mediterranean countries, such as Portugal, Spain, Tunisia, Algeria and Egypt, possibly due to livestock import/export trade between these countries. Moreover, Portugal presents a low risk of fascioliasis drug-resistance.Faculdade de Ciências e TecnologiaCalado, Maria ManuelaAfonso, AnaRUNSantos, Tânia Raquel Martins dos2013-02-05T10:49:22Z20122012-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10362/8689enginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T03:41:34Zoai:run.unl.pt:10362/8689Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:18:22.507434Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Genetic characterization of Portuguese Fasciola hepatica isolates
title Genetic characterization of Portuguese Fasciola hepatica isolates
spellingShingle Genetic characterization of Portuguese Fasciola hepatica isolates
Santos, Tânia Raquel Martins dos
Fasciola hepatica
genetic characterization
Portugal
cox1
nad1
ITS region
title_short Genetic characterization of Portuguese Fasciola hepatica isolates
title_full Genetic characterization of Portuguese Fasciola hepatica isolates
title_fullStr Genetic characterization of Portuguese Fasciola hepatica isolates
title_full_unstemmed Genetic characterization of Portuguese Fasciola hepatica isolates
title_sort Genetic characterization of Portuguese Fasciola hepatica isolates
author Santos, Tânia Raquel Martins dos
author_facet Santos, Tânia Raquel Martins dos
author_role author
dc.contributor.none.fl_str_mv Calado, Maria Manuela
Afonso, Ana
RUN
dc.contributor.author.fl_str_mv Santos, Tânia Raquel Martins dos
dc.subject.por.fl_str_mv Fasciola hepatica
genetic characterization
Portugal
cox1
nad1
ITS region
topic Fasciola hepatica
genetic characterization
Portugal
cox1
nad1
ITS region
description Dissertation presented to obtain the Master Degree in Molecular, Genetics and Biomedicine
publishDate 2012
dc.date.none.fl_str_mv 2012
2012-01-01T00:00:00Z
2013-02-05T10:49:22Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/8689
url http://hdl.handle.net/10362/8689
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Faculdade de Ciências e Tecnologia
publisher.none.fl_str_mv Faculdade de Ciências e Tecnologia
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799137829640470528

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