Selective cytotoxicity of Portuguese propolis ethyl acetate fraction towards renal cancer cells
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2022 |
| Outros Autores: | , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | https://hdl.handle.net/1822/80295 |
Resumo: | Renal cell carcinoma is the most lethal cancer of the urological system due to late diagnosis and treatment resistance. Propolis, a beehive product, is a valuable natural source of compounds with bioactivities and may be a beneficial addition to current anticancer treatments. A Portuguese propolis sample, its fractions (<i>n</i>-hexane, ethyl acetate, <i>n</i>-butanol and water) and three subfractions (<b>P1</b>–<b>P3</b>), were tested for their toxicity on A498, 786-O and Caki-2 renal cell carcinoma cell lines and the non-neoplastic HK2 kidney cells. The ethyl acetate fraction showed the strongest toxicity against A498 (IC<sub>50</sub> = 0.162 µg mL<sup>−1</sup>) and 786-O (IC<sub>50</sub> = 0.271 µg mL<sup>−1</sup>) cells. With similar toxicity against 786-O, <b>P1</b> (IC<sub>50</sub> = 3.8 µg mL<sup>−1</sup>) and <b>P3</b> (IC<sub>50</sub> = 3.1 µg mL<sup>−1</sup>) exhibited greater effect when combined (IC<sub>50</sub> = 2.5 µg mL<sup>−1</sup>). Results support the potential of propolis and its constituents as promising coadjuvants in renal cell carcinoma treatment. |
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Selective cytotoxicity of Portuguese propolis ethyl acetate fraction towards renal cancer cellsPropolisFractionationPhenolic compoundsPectolinarigeninCytotoxic activityRenal cell carcinomaScience & TechnologyRenal cell carcinoma is the most lethal cancer of the urological system due to late diagnosis and treatment resistance. Propolis, a beehive product, is a valuable natural source of compounds with bioactivities and may be a beneficial addition to current anticancer treatments. A Portuguese propolis sample, its fractions (<i>n</i>-hexane, ethyl acetate, <i>n</i>-butanol and water) and three subfractions (<b>P1</b>–<b>P3</b>), were tested for their toxicity on A498, 786-O and Caki-2 renal cell carcinoma cell lines and the non-neoplastic HK2 kidney cells. The ethyl acetate fraction showed the strongest toxicity against A498 (IC<sub>50</sub> = 0.162 µg mL<sup>−1</sup>) and 786-O (IC<sub>50</sub> = 0.271 µg mL<sup>−1</sup>) cells. With similar toxicity against 786-O, <b>P1</b> (IC<sub>50</sub> = 3.8 µg mL<sup>−1</sup>) and <b>P3</b> (IC<sub>50</sub> = 3.1 µg mL<sup>−1</sup>) exhibited greater effect when combined (IC<sub>50</sub> = 2.5 µg mL<sup>−1</sup>). Results support the potential of propolis and its constituents as promising coadjuvants in renal cell carcinoma treatment.Ana Freitas acknowledge the financial support provided by national funds through FCT-Portuguese Foundation for Science and Technology (PD/BD/128276/2017), under the Doctoral Programme “Agricultural Production Chains–from fork to farm” (PD/00122/2012) and from the European Social Funds and the Regional Operational Programme Norte 2020. This study was also supported by CITAB research unit (UIDB/04033/2020) and by the “Contrato-Programa” UIDB/04050/2020 funded by national funds through the FCT I.P. An acknowledge also to the University of Aveiro and to Fundação para a Ciência e a Tecnologia/Ministério da Ciência, Tecnologia e Ensino Superior (FCT/MCTES) for financial support to the associated laboratory LAQV-REQUIMTE (project reference UIDB/50006/2020), through national funds and co-financed by Fundo Europeu de Desenvolvimento Regional (FEDER), within the PT2020 Partnership Agreement. We also acknowledge the financial support from University of Minho, Fundação para a Ciência e a Tecnologia (FCT) and FEDERCOMPETE for financial support through Centro de Química (UID/QUI/00686/2013 and UID/QUI/0686/2016), for the PhD grant awarded to Olívia Pontes (SFRH/BD/128850/2017). The NMR spectrometer Bruker Avance III 400 is part of the National NMR Network (RNRMN) and was purchased within the framework of the National Program for Scientific Re-equipment, contract REDE/1517/RMN/2005 with funds from POCI 2010 (FEDER) and FCT. This work has been funded by National funds, through the Foundation for Science and Technology (FCT)-project UIDB/50026/2020 and UIDP/50026/2020.Multidisciplinary Digital Publishing Institute (MDPI)Universidade do MinhoFreitas, Ana SofiaCosta, Marta Sílvia FreitasPontes, OlíviaSeidel, VeroniqueProença, M. Fernanda R. P.Cardoso, Susana M.Oliveira, Rui Pedro Soares deBaltazar, FátimaAlmeida Aguiar, Cristina2022-06-222022-06-22T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/80295engFreitas, A.S.; Costa, M.; Pontes, O.; Seidel, V.; Proença, F.; Cardoso, S.M.; Oliveira, R.; Baltazar, F.; Almeida-Aguiar, C. Selective Cytotoxicity of Portuguese Propolis Ethyl Acetate Fraction towards Renal Cancer Cells. Molecules 2022, 27, 4001. https://doi.org/10.3390/molecules271340011420-304910.3390/molecules27134001358072474001https://www.mdpi.com/1420-3049/27/13/4001info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:47:05Zoai:repositorium.sdum.uminho.pt:1822/80295Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:45:09.152630Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
Selective cytotoxicity of Portuguese propolis ethyl acetate fraction towards renal cancer cells |
| title |
Selective cytotoxicity of Portuguese propolis ethyl acetate fraction towards renal cancer cells |
| spellingShingle |
Selective cytotoxicity of Portuguese propolis ethyl acetate fraction towards renal cancer cells Freitas, Ana Sofia Propolis Fractionation Phenolic compounds Pectolinarigenin Cytotoxic activity Renal cell carcinoma Science & Technology |
| title_short |
Selective cytotoxicity of Portuguese propolis ethyl acetate fraction towards renal cancer cells |
| title_full |
Selective cytotoxicity of Portuguese propolis ethyl acetate fraction towards renal cancer cells |
| title_fullStr |
Selective cytotoxicity of Portuguese propolis ethyl acetate fraction towards renal cancer cells |
| title_full_unstemmed |
Selective cytotoxicity of Portuguese propolis ethyl acetate fraction towards renal cancer cells |
| title_sort |
Selective cytotoxicity of Portuguese propolis ethyl acetate fraction towards renal cancer cells |
| author |
Freitas, Ana Sofia |
| author_facet |
Freitas, Ana Sofia Costa, Marta Sílvia Freitas Pontes, Olívia Seidel, Veronique Proença, M. Fernanda R. P. Cardoso, Susana M. Oliveira, Rui Pedro Soares de Baltazar, Fátima Almeida Aguiar, Cristina |
| author_role |
author |
| author2 |
Costa, Marta Sílvia Freitas Pontes, Olívia Seidel, Veronique Proença, M. Fernanda R. P. Cardoso, Susana M. Oliveira, Rui Pedro Soares de Baltazar, Fátima Almeida Aguiar, Cristina |
| author2_role |
author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Universidade do Minho |
| dc.contributor.author.fl_str_mv |
Freitas, Ana Sofia Costa, Marta Sílvia Freitas Pontes, Olívia Seidel, Veronique Proença, M. Fernanda R. P. Cardoso, Susana M. Oliveira, Rui Pedro Soares de Baltazar, Fátima Almeida Aguiar, Cristina |
| dc.subject.por.fl_str_mv |
Propolis Fractionation Phenolic compounds Pectolinarigenin Cytotoxic activity Renal cell carcinoma Science & Technology |
| topic |
Propolis Fractionation Phenolic compounds Pectolinarigenin Cytotoxic activity Renal cell carcinoma Science & Technology |
| description |
Renal cell carcinoma is the most lethal cancer of the urological system due to late diagnosis and treatment resistance. Propolis, a beehive product, is a valuable natural source of compounds with bioactivities and may be a beneficial addition to current anticancer treatments. A Portuguese propolis sample, its fractions (<i>n</i>-hexane, ethyl acetate, <i>n</i>-butanol and water) and three subfractions (<b>P1</b>–<b>P3</b>), were tested for their toxicity on A498, 786-O and Caki-2 renal cell carcinoma cell lines and the non-neoplastic HK2 kidney cells. The ethyl acetate fraction showed the strongest toxicity against A498 (IC<sub>50</sub> = 0.162 µg mL<sup>−1</sup>) and 786-O (IC<sub>50</sub> = 0.271 µg mL<sup>−1</sup>) cells. With similar toxicity against 786-O, <b>P1</b> (IC<sub>50</sub> = 3.8 µg mL<sup>−1</sup>) and <b>P3</b> (IC<sub>50</sub> = 3.1 µg mL<sup>−1</sup>) exhibited greater effect when combined (IC<sub>50</sub> = 2.5 µg mL<sup>−1</sup>). Results support the potential of propolis and its constituents as promising coadjuvants in renal cell carcinoma treatment. |
| publishDate |
2022 |
| dc.date.none.fl_str_mv |
2022-06-22 2022-06-22T00:00:00Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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https://hdl.handle.net/1822/80295 |
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https://hdl.handle.net/1822/80295 |
| dc.language.iso.fl_str_mv |
eng |
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eng |
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Freitas, A.S.; Costa, M.; Pontes, O.; Seidel, V.; Proença, F.; Cardoso, S.M.; Oliveira, R.; Baltazar, F.; Almeida-Aguiar, C. Selective Cytotoxicity of Portuguese Propolis Ethyl Acetate Fraction towards Renal Cancer Cells. Molecules 2022, 27, 4001. https://doi.org/10.3390/molecules27134001 1420-3049 10.3390/molecules27134001 35807247 4001 https://www.mdpi.com/1420-3049/27/13/4001 |
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openAccess |
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Multidisciplinary Digital Publishing Institute (MDPI) |
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Multidisciplinary Digital Publishing Institute (MDPI) |
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