Positive effects of antitumor drugs in combination with propolis on canine osteosarcoma cells (spOS-2) and mesenchymal stem cells

Detalhes bibliográficos
Autor(a) principal: Bernardino, Pedro Negri [UNESP]
Data de Publicação: 2018
Outros Autores: Bersano, Paulo Ricardo Oliveira, Lima Neto, João Ferreira, Sforcin, José Maurício [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.biopha.2018.05.027
http://hdl.handle.net/11449/179862
Resumo: The combination of lower concentrations of antitumor drugs (carboplatin – CARB, doxorubicin – DOX, and methotrexate – MET) with propolis was investigated against canine osteosarcoma (spOS-2) and mesenchymal stem cells (MSC) in vitro. The mechanism of action in the combinations was analyzed. spOS-2 cells were incubated up to 72 h with propolis (50 μg/ml) alone or in combination with CARB (10–400 μmol/l), DOX (0.5–2 μmol/l) or MET (50–200 μmol/l). Cell viability was assessed by MTT assay, apoptosis/necrosis by flow cytometry, and MSC was incubated with the optimum combination. Propolis alone exerted no cytotoxic action against spOS-2 cells, whereas CARB (400, 200 and 100 μmol/l) exhibited the highest cytotoxic effects comparing to DOX and MET. The combination of propolis with the lowest concentrations of CARB led to better results comparing to CARB alone, which was not observed using DOX and MET. Apoptosis was involved in the action of propolis + CARB in spOS-2 cells. MSC were not affected by CARB/propolis, indicating that the cytotoxic action of the combination was specific to tumor cells but not to normal ones. Propolis improved the action of CARB against spOS-2 cells using lower concentrations of this drug, without affecting MSC. These findings are relevant and indicate a possible application of propolis in OSA treatment.
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spelling Positive effects of antitumor drugs in combination with propolis on canine osteosarcoma cells (spOS-2) and mesenchymal stem cellsApoptosisCytotoxic actionOsteosarcomaPropolisThe combination of lower concentrations of antitumor drugs (carboplatin – CARB, doxorubicin – DOX, and methotrexate – MET) with propolis was investigated against canine osteosarcoma (spOS-2) and mesenchymal stem cells (MSC) in vitro. The mechanism of action in the combinations was analyzed. spOS-2 cells were incubated up to 72 h with propolis (50 μg/ml) alone or in combination with CARB (10–400 μmol/l), DOX (0.5–2 μmol/l) or MET (50–200 μmol/l). Cell viability was assessed by MTT assay, apoptosis/necrosis by flow cytometry, and MSC was incubated with the optimum combination. Propolis alone exerted no cytotoxic action against spOS-2 cells, whereas CARB (400, 200 and 100 μmol/l) exhibited the highest cytotoxic effects comparing to DOX and MET. The combination of propolis with the lowest concentrations of CARB led to better results comparing to CARB alone, which was not observed using DOX and MET. Apoptosis was involved in the action of propolis + CARB in spOS-2 cells. MSC were not affected by CARB/propolis, indicating that the cytotoxic action of the combination was specific to tumor cells but not to normal ones. Propolis improved the action of CARB against spOS-2 cells using lower concentrations of this drug, without affecting MSC. These findings are relevant and indicate a possible application of propolis in OSA treatment.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)São Paulo State University (UNESP) Institute of Biosciences, Campus BotucatuLaboratory of Pathology and Legal Veterinary Medicine Faculty of Veterinary Medicine State University of CearáPharmaceutical-Biochemistry Technology Department School of Pharmaceutical Science University of São PauloSão Paulo State University (UNESP) Institute of Biosciences, Campus BotucatuFAPESP: 2009/53493-9FAPESP: 2012/21158-9Universidade Estadual Paulista (Unesp)State University of CearáUniversidade de São Paulo (USP)Bernardino, Pedro Negri [UNESP]Bersano, Paulo Ricardo OliveiraLima Neto, João FerreiraSforcin, José Maurício [UNESP]2018-12-11T17:37:04Z2018-12-11T17:37:04Z2018-08-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article268-274application/pdfhttp://dx.doi.org/10.1016/j.biopha.2018.05.027Biomedicine and Pharmacotherapy, v. 104, p. 268-274.1950-60070753-3322http://hdl.handle.net/11449/17986210.1016/j.biopha.2018.05.0272-s2.0-850470073632-s2.0-85047007363.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBiomedicine and Pharmacotherapy0,951info:eu-repo/semantics/openAccess2023-11-20T06:14:47Zoai:repositorio.unesp.br:11449/179862Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-11-20T06:14:47Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Positive effects of antitumor drugs in combination with propolis on canine osteosarcoma cells (spOS-2) and mesenchymal stem cells
title Positive effects of antitumor drugs in combination with propolis on canine osteosarcoma cells (spOS-2) and mesenchymal stem cells
spellingShingle Positive effects of antitumor drugs in combination with propolis on canine osteosarcoma cells (spOS-2) and mesenchymal stem cells
Bernardino, Pedro Negri [UNESP]
Apoptosis
Cytotoxic action
Osteosarcoma
Propolis
title_short Positive effects of antitumor drugs in combination with propolis on canine osteosarcoma cells (spOS-2) and mesenchymal stem cells
title_full Positive effects of antitumor drugs in combination with propolis on canine osteosarcoma cells (spOS-2) and mesenchymal stem cells
title_fullStr Positive effects of antitumor drugs in combination with propolis on canine osteosarcoma cells (spOS-2) and mesenchymal stem cells
title_full_unstemmed Positive effects of antitumor drugs in combination with propolis on canine osteosarcoma cells (spOS-2) and mesenchymal stem cells
title_sort Positive effects of antitumor drugs in combination with propolis on canine osteosarcoma cells (spOS-2) and mesenchymal stem cells
author Bernardino, Pedro Negri [UNESP]
author_facet Bernardino, Pedro Negri [UNESP]
Bersano, Paulo Ricardo Oliveira
Lima Neto, João Ferreira
Sforcin, José Maurício [UNESP]
author_role author
author2 Bersano, Paulo Ricardo Oliveira
Lima Neto, João Ferreira
Sforcin, José Maurício [UNESP]
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
State University of Ceará
Universidade de São Paulo (USP)
dc.contributor.author.fl_str_mv Bernardino, Pedro Negri [UNESP]
Bersano, Paulo Ricardo Oliveira
Lima Neto, João Ferreira
Sforcin, José Maurício [UNESP]
dc.subject.por.fl_str_mv Apoptosis
Cytotoxic action
Osteosarcoma
Propolis
topic Apoptosis
Cytotoxic action
Osteosarcoma
Propolis
description The combination of lower concentrations of antitumor drugs (carboplatin – CARB, doxorubicin – DOX, and methotrexate – MET) with propolis was investigated against canine osteosarcoma (spOS-2) and mesenchymal stem cells (MSC) in vitro. The mechanism of action in the combinations was analyzed. spOS-2 cells were incubated up to 72 h with propolis (50 μg/ml) alone or in combination with CARB (10–400 μmol/l), DOX (0.5–2 μmol/l) or MET (50–200 μmol/l). Cell viability was assessed by MTT assay, apoptosis/necrosis by flow cytometry, and MSC was incubated with the optimum combination. Propolis alone exerted no cytotoxic action against spOS-2 cells, whereas CARB (400, 200 and 100 μmol/l) exhibited the highest cytotoxic effects comparing to DOX and MET. The combination of propolis with the lowest concentrations of CARB led to better results comparing to CARB alone, which was not observed using DOX and MET. Apoptosis was involved in the action of propolis + CARB in spOS-2 cells. MSC were not affected by CARB/propolis, indicating that the cytotoxic action of the combination was specific to tumor cells but not to normal ones. Propolis improved the action of CARB against spOS-2 cells using lower concentrations of this drug, without affecting MSC. These findings are relevant and indicate a possible application of propolis in OSA treatment.
publishDate 2018
dc.date.none.fl_str_mv 2018-12-11T17:37:04Z
2018-12-11T17:37:04Z
2018-08-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.biopha.2018.05.027
Biomedicine and Pharmacotherapy, v. 104, p. 268-274.
1950-6007
0753-3322
http://hdl.handle.net/11449/179862
10.1016/j.biopha.2018.05.027
2-s2.0-85047007363
2-s2.0-85047007363.pdf
url http://dx.doi.org/10.1016/j.biopha.2018.05.027
http://hdl.handle.net/11449/179862
identifier_str_mv Biomedicine and Pharmacotherapy, v. 104, p. 268-274.
1950-6007
0753-3322
10.1016/j.biopha.2018.05.027
2-s2.0-85047007363
2-s2.0-85047007363.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Biomedicine and Pharmacotherapy
0,951
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 268-274
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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