Quercus suber Roots Activate Antioxidant and Membrane Protective Processes in Response to High Salinity
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10316/103462 https://doi.org/10.3390/plants11040557 |
Resumo: | Cork oak (Quercus suber) is a species native to Mediterranean areas and its adaptation to the increasingly prevalent abiotic stresses, such as soil salinization, remain unknown. In sequence with recent studies on salt stress response in the leaf, it is fundamental to uncover the plasticity of roots directly exposed to high salinity to better understand how Q. suber copes with salt stress. In the present study we aimed to unveil the antioxidants and key-genes involved in the stress-responses (early vs. later responses) of Q. suber roots exposed to high salinity. Two-month-old Q. suber plants were watered with 300 mM NaCl solution and enzymatic and non-enzymatic antioxidants, lipid peroxidation and the relative expression of genes related to stress response were analysed 8 h and 6 days after salt treatment. After an 8 h of exposure, roots activated the expression of QsLTI30 and QsFAD7 genes involved in stress membrane protection, and QsRAV1 and QsCZF1 genes involved in tolerance and adaptation. As a result of the continued salinity stress (6 days), lipid peroxidation increased, which was associated with an upregulation of QsLTI30 gene. Moreover, other protective mechanisms were activated, such as the upregulation of genes related to antioxidant status, QsCSD1 and QsAPX2, and the increase of the antioxidant enzyme activities of superoxide dismutase, catalase, and ascorbate peroxidase, concomitantly with total antioxidant activity and phenols. These data suggest a response dependent on the time of salinity exposure, leading Q. suber roots to adopt protective complementary strategies to deal with salt stress. |
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Quercus suber Roots Activate Antioxidant and Membrane Protective Processes in Response to High Salinitysalinizationoxidative stressmembrane protectionAP2/ERF family transcription factorszinc finger CCCH domain-containing proteinsdehydrinsCork oak (Quercus suber) is a species native to Mediterranean areas and its adaptation to the increasingly prevalent abiotic stresses, such as soil salinization, remain unknown. In sequence with recent studies on salt stress response in the leaf, it is fundamental to uncover the plasticity of roots directly exposed to high salinity to better understand how Q. suber copes with salt stress. In the present study we aimed to unveil the antioxidants and key-genes involved in the stress-responses (early vs. later responses) of Q. suber roots exposed to high salinity. Two-month-old Q. suber plants were watered with 300 mM NaCl solution and enzymatic and non-enzymatic antioxidants, lipid peroxidation and the relative expression of genes related to stress response were analysed 8 h and 6 days after salt treatment. After an 8 h of exposure, roots activated the expression of QsLTI30 and QsFAD7 genes involved in stress membrane protection, and QsRAV1 and QsCZF1 genes involved in tolerance and adaptation. As a result of the continued salinity stress (6 days), lipid peroxidation increased, which was associated with an upregulation of QsLTI30 gene. Moreover, other protective mechanisms were activated, such as the upregulation of genes related to antioxidant status, QsCSD1 and QsAPX2, and the increase of the antioxidant enzyme activities of superoxide dismutase, catalase, and ascorbate peroxidase, concomitantly with total antioxidant activity and phenols. These data suggest a response dependent on the time of salinity exposure, leading Q. suber roots to adopt protective complementary strategies to deal with salt stress.MDPI2022-02-19info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10316/103462http://hdl.handle.net/10316/103462https://doi.org/10.3390/plants11040557eng2223-7747Dias, Maria CelesteSantos, ConceiçãoAraújo, MárciaBarros, Pedro M.Oliveira, MargaridaOliveira, José Miguel P. Ferreira deinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-11-14T21:35:49Zoai:estudogeral.uc.pt:10316/103462Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:20:17.688897Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Quercus suber Roots Activate Antioxidant and Membrane Protective Processes in Response to High Salinity |
title |
Quercus suber Roots Activate Antioxidant and Membrane Protective Processes in Response to High Salinity |
spellingShingle |
Quercus suber Roots Activate Antioxidant and Membrane Protective Processes in Response to High Salinity Dias, Maria Celeste salinization oxidative stress membrane protection AP2/ERF family transcription factors zinc finger CCCH domain-containing proteins dehydrins |
title_short |
Quercus suber Roots Activate Antioxidant and Membrane Protective Processes in Response to High Salinity |
title_full |
Quercus suber Roots Activate Antioxidant and Membrane Protective Processes in Response to High Salinity |
title_fullStr |
Quercus suber Roots Activate Antioxidant and Membrane Protective Processes in Response to High Salinity |
title_full_unstemmed |
Quercus suber Roots Activate Antioxidant and Membrane Protective Processes in Response to High Salinity |
title_sort |
Quercus suber Roots Activate Antioxidant and Membrane Protective Processes in Response to High Salinity |
author |
Dias, Maria Celeste |
author_facet |
Dias, Maria Celeste Santos, Conceição Araújo, Márcia Barros, Pedro M. Oliveira, Margarida Oliveira, José Miguel P. Ferreira de |
author_role |
author |
author2 |
Santos, Conceição Araújo, Márcia Barros, Pedro M. Oliveira, Margarida Oliveira, José Miguel P. Ferreira de |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Dias, Maria Celeste Santos, Conceição Araújo, Márcia Barros, Pedro M. Oliveira, Margarida Oliveira, José Miguel P. Ferreira de |
dc.subject.por.fl_str_mv |
salinization oxidative stress membrane protection AP2/ERF family transcription factors zinc finger CCCH domain-containing proteins dehydrins |
topic |
salinization oxidative stress membrane protection AP2/ERF family transcription factors zinc finger CCCH domain-containing proteins dehydrins |
description |
Cork oak (Quercus suber) is a species native to Mediterranean areas and its adaptation to the increasingly prevalent abiotic stresses, such as soil salinization, remain unknown. In sequence with recent studies on salt stress response in the leaf, it is fundamental to uncover the plasticity of roots directly exposed to high salinity to better understand how Q. suber copes with salt stress. In the present study we aimed to unveil the antioxidants and key-genes involved in the stress-responses (early vs. later responses) of Q. suber roots exposed to high salinity. Two-month-old Q. suber plants were watered with 300 mM NaCl solution and enzymatic and non-enzymatic antioxidants, lipid peroxidation and the relative expression of genes related to stress response were analysed 8 h and 6 days after salt treatment. After an 8 h of exposure, roots activated the expression of QsLTI30 and QsFAD7 genes involved in stress membrane protection, and QsRAV1 and QsCZF1 genes involved in tolerance and adaptation. As a result of the continued salinity stress (6 days), lipid peroxidation increased, which was associated with an upregulation of QsLTI30 gene. Moreover, other protective mechanisms were activated, such as the upregulation of genes related to antioxidant status, QsCSD1 and QsAPX2, and the increase of the antioxidant enzyme activities of superoxide dismutase, catalase, and ascorbate peroxidase, concomitantly with total antioxidant activity and phenols. These data suggest a response dependent on the time of salinity exposure, leading Q. suber roots to adopt protective complementary strategies to deal with salt stress. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-02-19 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10316/103462 http://hdl.handle.net/10316/103462 https://doi.org/10.3390/plants11040557 |
url |
http://hdl.handle.net/10316/103462 https://doi.org/10.3390/plants11040557 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
2223-7747 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
MDPI |
publisher.none.fl_str_mv |
MDPI |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799134095857418240 |