Quercus suber Roots Activate Antioxidant and Membrane Protective Processes in Response to High Salinity

Detalhes bibliográficos
Autor(a) principal: Dias, Maria Celeste
Data de Publicação: 2022
Outros Autores: Santos, Conceição, Araújo, Márcia, Barros, Pedro M., Oliveira, Margarida, Oliveira, José Miguel P. Ferreira de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10316/103462
https://doi.org/10.3390/plants11040557
Resumo: Cork oak (Quercus suber) is a species native to Mediterranean areas and its adaptation to the increasingly prevalent abiotic stresses, such as soil salinization, remain unknown. In sequence with recent studies on salt stress response in the leaf, it is fundamental to uncover the plasticity of roots directly exposed to high salinity to better understand how Q. suber copes with salt stress. In the present study we aimed to unveil the antioxidants and key-genes involved in the stress-responses (early vs. later responses) of Q. suber roots exposed to high salinity. Two-month-old Q. suber plants were watered with 300 mM NaCl solution and enzymatic and non-enzymatic antioxidants, lipid peroxidation and the relative expression of genes related to stress response were analysed 8 h and 6 days after salt treatment. After an 8 h of exposure, roots activated the expression of QsLTI30 and QsFAD7 genes involved in stress membrane protection, and QsRAV1 and QsCZF1 genes involved in tolerance and adaptation. As a result of the continued salinity stress (6 days), lipid peroxidation increased, which was associated with an upregulation of QsLTI30 gene. Moreover, other protective mechanisms were activated, such as the upregulation of genes related to antioxidant status, QsCSD1 and QsAPX2, and the increase of the antioxidant enzyme activities of superoxide dismutase, catalase, and ascorbate peroxidase, concomitantly with total antioxidant activity and phenols. These data suggest a response dependent on the time of salinity exposure, leading Q. suber roots to adopt protective complementary strategies to deal with salt stress.
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spelling Quercus suber Roots Activate Antioxidant and Membrane Protective Processes in Response to High Salinitysalinizationoxidative stressmembrane protectionAP2/ERF family transcription factorszinc finger CCCH domain-containing proteinsdehydrinsCork oak (Quercus suber) is a species native to Mediterranean areas and its adaptation to the increasingly prevalent abiotic stresses, such as soil salinization, remain unknown. In sequence with recent studies on salt stress response in the leaf, it is fundamental to uncover the plasticity of roots directly exposed to high salinity to better understand how Q. suber copes with salt stress. In the present study we aimed to unveil the antioxidants and key-genes involved in the stress-responses (early vs. later responses) of Q. suber roots exposed to high salinity. Two-month-old Q. suber plants were watered with 300 mM NaCl solution and enzymatic and non-enzymatic antioxidants, lipid peroxidation and the relative expression of genes related to stress response were analysed 8 h and 6 days after salt treatment. After an 8 h of exposure, roots activated the expression of QsLTI30 and QsFAD7 genes involved in stress membrane protection, and QsRAV1 and QsCZF1 genes involved in tolerance and adaptation. As a result of the continued salinity stress (6 days), lipid peroxidation increased, which was associated with an upregulation of QsLTI30 gene. Moreover, other protective mechanisms were activated, such as the upregulation of genes related to antioxidant status, QsCSD1 and QsAPX2, and the increase of the antioxidant enzyme activities of superoxide dismutase, catalase, and ascorbate peroxidase, concomitantly with total antioxidant activity and phenols. These data suggest a response dependent on the time of salinity exposure, leading Q. suber roots to adopt protective complementary strategies to deal with salt stress.MDPI2022-02-19info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10316/103462http://hdl.handle.net/10316/103462https://doi.org/10.3390/plants11040557eng2223-7747Dias, Maria CelesteSantos, ConceiçãoAraújo, MárciaBarros, Pedro M.Oliveira, MargaridaOliveira, José Miguel P. Ferreira deinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-11-14T21:35:49Zoai:estudogeral.uc.pt:10316/103462Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:20:17.688897Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Quercus suber Roots Activate Antioxidant and Membrane Protective Processes in Response to High Salinity
title Quercus suber Roots Activate Antioxidant and Membrane Protective Processes in Response to High Salinity
spellingShingle Quercus suber Roots Activate Antioxidant and Membrane Protective Processes in Response to High Salinity
Dias, Maria Celeste
salinization
oxidative stress
membrane protection
AP2/ERF family transcription factors
zinc finger CCCH domain-containing proteins
dehydrins
title_short Quercus suber Roots Activate Antioxidant and Membrane Protective Processes in Response to High Salinity
title_full Quercus suber Roots Activate Antioxidant and Membrane Protective Processes in Response to High Salinity
title_fullStr Quercus suber Roots Activate Antioxidant and Membrane Protective Processes in Response to High Salinity
title_full_unstemmed Quercus suber Roots Activate Antioxidant and Membrane Protective Processes in Response to High Salinity
title_sort Quercus suber Roots Activate Antioxidant and Membrane Protective Processes in Response to High Salinity
author Dias, Maria Celeste
author_facet Dias, Maria Celeste
Santos, Conceição
Araújo, Márcia
Barros, Pedro M.
Oliveira, Margarida
Oliveira, José Miguel P. Ferreira de
author_role author
author2 Santos, Conceição
Araújo, Márcia
Barros, Pedro M.
Oliveira, Margarida
Oliveira, José Miguel P. Ferreira de
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Dias, Maria Celeste
Santos, Conceição
Araújo, Márcia
Barros, Pedro M.
Oliveira, Margarida
Oliveira, José Miguel P. Ferreira de
dc.subject.por.fl_str_mv salinization
oxidative stress
membrane protection
AP2/ERF family transcription factors
zinc finger CCCH domain-containing proteins
dehydrins
topic salinization
oxidative stress
membrane protection
AP2/ERF family transcription factors
zinc finger CCCH domain-containing proteins
dehydrins
description Cork oak (Quercus suber) is a species native to Mediterranean areas and its adaptation to the increasingly prevalent abiotic stresses, such as soil salinization, remain unknown. In sequence with recent studies on salt stress response in the leaf, it is fundamental to uncover the plasticity of roots directly exposed to high salinity to better understand how Q. suber copes with salt stress. In the present study we aimed to unveil the antioxidants and key-genes involved in the stress-responses (early vs. later responses) of Q. suber roots exposed to high salinity. Two-month-old Q. suber plants were watered with 300 mM NaCl solution and enzymatic and non-enzymatic antioxidants, lipid peroxidation and the relative expression of genes related to stress response were analysed 8 h and 6 days after salt treatment. After an 8 h of exposure, roots activated the expression of QsLTI30 and QsFAD7 genes involved in stress membrane protection, and QsRAV1 and QsCZF1 genes involved in tolerance and adaptation. As a result of the continued salinity stress (6 days), lipid peroxidation increased, which was associated with an upregulation of QsLTI30 gene. Moreover, other protective mechanisms were activated, such as the upregulation of genes related to antioxidant status, QsCSD1 and QsAPX2, and the increase of the antioxidant enzyme activities of superoxide dismutase, catalase, and ascorbate peroxidase, concomitantly with total antioxidant activity and phenols. These data suggest a response dependent on the time of salinity exposure, leading Q. suber roots to adopt protective complementary strategies to deal with salt stress.
publishDate 2022
dc.date.none.fl_str_mv 2022-02-19
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10316/103462
http://hdl.handle.net/10316/103462
https://doi.org/10.3390/plants11040557
url http://hdl.handle.net/10316/103462
https://doi.org/10.3390/plants11040557
dc.language.iso.fl_str_mv eng
language eng
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dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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