Toward a platform for the treatment of burns: An assessment of nanoemulsions vs. nanostructured lipid carriers loaded with curcumin

Detalhes bibliográficos
Autor(a) principal: Araújo, Gabriela de Moraes Soares
Data de Publicação: 2023
Outros Autores: Loureiro, Ana Isabel Sá, Rodrigues, Jamile Lima, Barros, Paula Alice Bezerra, Halicki, Priscila Cristina Bartolomeu, Ramos, Daniela Fernandes, Marinho, Marcelo Augusto Germani, Vaiss, Daniela Pastorim, Vaz, Gustavo Richter, Yurgel, Virginia Campello, Bidone, Juliana, Muccillo-Baisch, Ana Luiza, Hort, Mariana Appel, Cavaco-Paulo, Artur, Dora, Cristiana Lima
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://hdl.handle.net/1822/91451
Resumo: Curcumin is a highly promising substance for treating burns, owing to its anti-inflammatory, antioxidant, antimicrobial, and wound-healing properties. However, its therapeutic use is restricted due to its hydrophobic nature and low bioavailability. This study was conducted to address these limitations; it developed and tested two types of lipid nanocarriers, namely nanoemulsions (NE-CUR) and nanostructured lipid carriers (NLC-CUR) loaded with curcumin, and aimed to identify the most suitable nanocarrier for skin burn treatment. The study evaluated various parameters, including physicochemical characteristics, stability, encapsulation efficiency, release, skin permeation, retention, cell viability, and antimicrobial activity. The results showed that both nanocarriers showed adequate size (~200 nm), polydispersity index (~0.25), and zeta potential (~>20 mV). They also showed good encapsulation efficiency (>90%) and remained stable for 120 days at different temperatures. In the release test, NE-CUR and NCL-CUR released 57.14% and 51.64% of curcumin, respectively, in 72 h. NE-CUR demonstrated better cutaneous permeation/retention in intact or scalded skin epidermis and dermis than NLC-CUR. The cell viability test showed no toxicity after treatment with NE-CUR and NLC-CUR up to 125 g/mL. Regarding microbial activity assays, free curcumin has activity against P. aeruginosa, reducing bacterial growth by 75% in 3 h. NE-CUR inhibited bacterial growth by 65% after 24 h, and the association with gentamicin had favorable results, while NLC-CUR showed a lower inhibition. The results demonstrated that NE-CUR is probably the most promising nanocarrier for treating burns.
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spelling Toward a platform for the treatment of burns: An assessment of nanoemulsions vs. nanostructured lipid carriers loaded with curcuminBurnNanotechnologyCurcuminNanoemulsionNanostructured lipid carrierCurcumin is a highly promising substance for treating burns, owing to its anti-inflammatory, antioxidant, antimicrobial, and wound-healing properties. However, its therapeutic use is restricted due to its hydrophobic nature and low bioavailability. This study was conducted to address these limitations; it developed and tested two types of lipid nanocarriers, namely nanoemulsions (NE-CUR) and nanostructured lipid carriers (NLC-CUR) loaded with curcumin, and aimed to identify the most suitable nanocarrier for skin burn treatment. The study evaluated various parameters, including physicochemical characteristics, stability, encapsulation efficiency, release, skin permeation, retention, cell viability, and antimicrobial activity. The results showed that both nanocarriers showed adequate size (~200 nm), polydispersity index (~0.25), and zeta potential (~>20 mV). They also showed good encapsulation efficiency (>90%) and remained stable for 120 days at different temperatures. In the release test, NE-CUR and NCL-CUR released 57.14% and 51.64% of curcumin, respectively, in 72 h. NE-CUR demonstrated better cutaneous permeation/retention in intact or scalded skin epidermis and dermis than NLC-CUR. The cell viability test showed no toxicity after treatment with NE-CUR and NLC-CUR up to 125 g/mL. Regarding microbial activity assays, free curcumin has activity against P. aeruginosa, reducing bacterial growth by 75% in 3 h. NE-CUR inhibited bacterial growth by 65% after 24 h, and the association with gentamicin had favorable results, while NLC-CUR showed a lower inhibition. The results demonstrated that NE-CUR is probably the most promising nanocarrier for treating burns.FCT -Fundação para a Ciência e a Tecnologia(2022.13494)info:eu-repo/semantics/publishedVersionMDPI AGUniversidade do MinhoAraújo, Gabriela de Moraes SoaresLoureiro, Ana Isabel SáRodrigues, Jamile LimaBarros, Paula Alice BezerraHalicki, Priscila Cristina BartolomeuRamos, Daniela FernandesMarinho, Marcelo Augusto GermaniVaiss, Daniela PastorimVaz, Gustavo RichterYurgel, Virginia CampelloBidone, JulianaMuccillo-Baisch, Ana LuizaHort, Mariana AppelCavaco-Paulo, ArturDora, Cristiana Lima20232023-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/91451engAraújo, Gabriela de Moraes Soares; Loureiro, Ana; Rodrigues, Jamile Lima; Barros, Paula Alice Bezerra; Halicki, Priscila Cristina Bartolomeu; Ramos, Daniela Fernandes; Marinho, Marcelo Augusto Germani; Vaiss, Daniela Pastorim; Vaz, Gustavo Richter; Yurgel, Virginia Campello; Bidone, Juliana; Muccillo-Baisch, Ana Luiza; Hort, Mariana Appel; Cavaco-Paulo, Artur; Dora, Cristiana Lima, Toward a platform for the treatment of burns: an assessment of nanoemulsions vs. nanostructured lipid carriers loaded with curcumin. Biomedicines, 11(12), 3348, 20232227-905910.3390/biomedicines11123348https://www.mdpi.com/journal/biomedicinesinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-05-25T02:15:19Zoai:repositorium.sdum.uminho.pt:1822/91451Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-05-25T02:15:19Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Toward a platform for the treatment of burns: An assessment of nanoemulsions vs. nanostructured lipid carriers loaded with curcumin
title Toward a platform for the treatment of burns: An assessment of nanoemulsions vs. nanostructured lipid carriers loaded with curcumin
spellingShingle Toward a platform for the treatment of burns: An assessment of nanoemulsions vs. nanostructured lipid carriers loaded with curcumin
Araújo, Gabriela de Moraes Soares
Burn
Nanotechnology
Curcumin
Nanoemulsion
Nanostructured lipid carrier
title_short Toward a platform for the treatment of burns: An assessment of nanoemulsions vs. nanostructured lipid carriers loaded with curcumin
title_full Toward a platform for the treatment of burns: An assessment of nanoemulsions vs. nanostructured lipid carriers loaded with curcumin
title_fullStr Toward a platform for the treatment of burns: An assessment of nanoemulsions vs. nanostructured lipid carriers loaded with curcumin
title_full_unstemmed Toward a platform for the treatment of burns: An assessment of nanoemulsions vs. nanostructured lipid carriers loaded with curcumin
title_sort Toward a platform for the treatment of burns: An assessment of nanoemulsions vs. nanostructured lipid carriers loaded with curcumin
author Araújo, Gabriela de Moraes Soares
author_facet Araújo, Gabriela de Moraes Soares
Loureiro, Ana Isabel Sá
Rodrigues, Jamile Lima
Barros, Paula Alice Bezerra
Halicki, Priscila Cristina Bartolomeu
Ramos, Daniela Fernandes
Marinho, Marcelo Augusto Germani
Vaiss, Daniela Pastorim
Vaz, Gustavo Richter
Yurgel, Virginia Campello
Bidone, Juliana
Muccillo-Baisch, Ana Luiza
Hort, Mariana Appel
Cavaco-Paulo, Artur
Dora, Cristiana Lima
author_role author
author2 Loureiro, Ana Isabel Sá
Rodrigues, Jamile Lima
Barros, Paula Alice Bezerra
Halicki, Priscila Cristina Bartolomeu
Ramos, Daniela Fernandes
Marinho, Marcelo Augusto Germani
Vaiss, Daniela Pastorim
Vaz, Gustavo Richter
Yurgel, Virginia Campello
Bidone, Juliana
Muccillo-Baisch, Ana Luiza
Hort, Mariana Appel
Cavaco-Paulo, Artur
Dora, Cristiana Lima
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Araújo, Gabriela de Moraes Soares
Loureiro, Ana Isabel Sá
Rodrigues, Jamile Lima
Barros, Paula Alice Bezerra
Halicki, Priscila Cristina Bartolomeu
Ramos, Daniela Fernandes
Marinho, Marcelo Augusto Germani
Vaiss, Daniela Pastorim
Vaz, Gustavo Richter
Yurgel, Virginia Campello
Bidone, Juliana
Muccillo-Baisch, Ana Luiza
Hort, Mariana Appel
Cavaco-Paulo, Artur
Dora, Cristiana Lima
dc.subject.por.fl_str_mv Burn
Nanotechnology
Curcumin
Nanoemulsion
Nanostructured lipid carrier
topic Burn
Nanotechnology
Curcumin
Nanoemulsion
Nanostructured lipid carrier
description Curcumin is a highly promising substance for treating burns, owing to its anti-inflammatory, antioxidant, antimicrobial, and wound-healing properties. However, its therapeutic use is restricted due to its hydrophobic nature and low bioavailability. This study was conducted to address these limitations; it developed and tested two types of lipid nanocarriers, namely nanoemulsions (NE-CUR) and nanostructured lipid carriers (NLC-CUR) loaded with curcumin, and aimed to identify the most suitable nanocarrier for skin burn treatment. The study evaluated various parameters, including physicochemical characteristics, stability, encapsulation efficiency, release, skin permeation, retention, cell viability, and antimicrobial activity. The results showed that both nanocarriers showed adequate size (~200 nm), polydispersity index (~0.25), and zeta potential (~>20 mV). They also showed good encapsulation efficiency (>90%) and remained stable for 120 days at different temperatures. In the release test, NE-CUR and NCL-CUR released 57.14% and 51.64% of curcumin, respectively, in 72 h. NE-CUR demonstrated better cutaneous permeation/retention in intact or scalded skin epidermis and dermis than NLC-CUR. The cell viability test showed no toxicity after treatment with NE-CUR and NLC-CUR up to 125 g/mL. Regarding microbial activity assays, free curcumin has activity against P. aeruginosa, reducing bacterial growth by 75% in 3 h. NE-CUR inhibited bacterial growth by 65% after 24 h, and the association with gentamicin had favorable results, while NLC-CUR showed a lower inhibition. The results demonstrated that NE-CUR is probably the most promising nanocarrier for treating burns.
publishDate 2023
dc.date.none.fl_str_mv 2023
2023-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://hdl.handle.net/1822/91451
url https://hdl.handle.net/1822/91451
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Araújo, Gabriela de Moraes Soares; Loureiro, Ana; Rodrigues, Jamile Lima; Barros, Paula Alice Bezerra; Halicki, Priscila Cristina Bartolomeu; Ramos, Daniela Fernandes; Marinho, Marcelo Augusto Germani; Vaiss, Daniela Pastorim; Vaz, Gustavo Richter; Yurgel, Virginia Campello; Bidone, Juliana; Muccillo-Baisch, Ana Luiza; Hort, Mariana Appel; Cavaco-Paulo, Artur; Dora, Cristiana Lima, Toward a platform for the treatment of burns: an assessment of nanoemulsions vs. nanostructured lipid carriers loaded with curcumin. Biomedicines, 11(12), 3348, 2023
2227-9059
10.3390/biomedicines11123348
https://www.mdpi.com/journal/biomedicines
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI AG
publisher.none.fl_str_mv MDPI AG
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv mluisa.alvim@gmail.com
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