Validation of an innovative analytical method for simultaneous quantification of curcumin and fluconazole using high-performance liquid chromatography from nanostructured lipid carriers
Autor(a) principal: | |
---|---|
Data de Publicação: | 2021 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1002/jssc.202100514 http://hdl.handle.net/11449/229658 |
Resumo: | Vulvovaginal candidiasis is a public health problem with a high incidence among female patients. Currently, there is an increase in the identification of Candida spp. resistant to current therapy, making it necessary to search for new therapeutic alternatives. The synergistic potential of curcumin with fluconazole is described in the literature. However, due to its high lipophilicity, it is necessary to use drug-delivery systems to adequately explore its potential, among which is the nanostructured lipid carrier. However, to date, there is no validated method of high-performance liquid chromatography for simultaneous determination of fluconazole and curcumin in the literature. Thus, the present work developed a high-performance liquid chromatography method for simultaneous determination of fluconazole and curcumin co-encapsulated in nanostructured lipid carrier which was validated according to the International Conference on Harmonization (Technical Requirements for Registration of Pharmaceuticals for Human Use) – Q2 (R1) and the Food and Drug Administration - Guidance for Bioanalytical Method. The method was applied to determine the encapsulation efficiency and drug-loading of curcumin and fluconazole in nanostructured lipid carriers. The developed method proved to be selective, precise, accurate, and robust for the simultaneous determination of both drugs, enabling the quantification of encapsulation efficiency and drug-loading of curcumin and fluconazole in nanostructured lipid carriers. |
id |
UNSP_e0ba0bb760650ab38815c8e39041a750 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/229658 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Validation of an innovative analytical method for simultaneous quantification of curcumin and fluconazole using high-performance liquid chromatography from nanostructured lipid carrierscurcuminfluconazolenanostructured lipid carrierssimultaneous detectionVulvovaginal candidiasis is a public health problem with a high incidence among female patients. Currently, there is an increase in the identification of Candida spp. resistant to current therapy, making it necessary to search for new therapeutic alternatives. The synergistic potential of curcumin with fluconazole is described in the literature. However, due to its high lipophilicity, it is necessary to use drug-delivery systems to adequately explore its potential, among which is the nanostructured lipid carrier. However, to date, there is no validated method of high-performance liquid chromatography for simultaneous determination of fluconazole and curcumin in the literature. Thus, the present work developed a high-performance liquid chromatography method for simultaneous determination of fluconazole and curcumin co-encapsulated in nanostructured lipid carrier which was validated according to the International Conference on Harmonization (Technical Requirements for Registration of Pharmaceuticals for Human Use) – Q2 (R1) and the Food and Drug Administration - Guidance for Bioanalytical Method. The method was applied to determine the encapsulation efficiency and drug-loading of curcumin and fluconazole in nanostructured lipid carriers. The developed method proved to be selective, precise, accurate, and robust for the simultaneous determination of both drugs, enabling the quantification of encapsulation efficiency and drug-loading of curcumin and fluconazole in nanostructured lipid carriers.School of Pharmaceutical Sciences São Paulo State UniversityDepartment of Drugs and Medicines School of Pharmaceutical Sciences São Paulo State UniversitySchool of Pharmaceutical Sciences São Paulo State UniversityDepartment of Drugs and Medicines School of Pharmaceutical Sciences São Paulo State UniversityUniversidade Estadual Paulista (UNESP)Araujo, Victor Hugo Sousa [UNESP]Fernandes, Lígia de Souza [UNESP]dos Reis, Leidiana Rocha [UNESP]Carvalho, Gabriela Corrêa [UNESP]Scarpa, Maria Virginia [UNESP]Chorilli, Marlus [UNESP]2022-04-29T08:34:59Z2022-04-29T08:34:59Z2021-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1002/jssc.202100514Journal of Separation Science.1615-93141615-9306http://hdl.handle.net/11449/22965810.1002/jssc.2021005142-s2.0-85116518805Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Separation Scienceinfo:eu-repo/semantics/openAccess2022-04-29T08:35:00Zoai:repositorio.unesp.br:11449/229658Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462022-04-29T08:35Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Validation of an innovative analytical method for simultaneous quantification of curcumin and fluconazole using high-performance liquid chromatography from nanostructured lipid carriers |
title |
Validation of an innovative analytical method for simultaneous quantification of curcumin and fluconazole using high-performance liquid chromatography from nanostructured lipid carriers |
spellingShingle |
Validation of an innovative analytical method for simultaneous quantification of curcumin and fluconazole using high-performance liquid chromatography from nanostructured lipid carriers Araujo, Victor Hugo Sousa [UNESP] curcumin fluconazole nanostructured lipid carriers simultaneous detection |
title_short |
Validation of an innovative analytical method for simultaneous quantification of curcumin and fluconazole using high-performance liquid chromatography from nanostructured lipid carriers |
title_full |
Validation of an innovative analytical method for simultaneous quantification of curcumin and fluconazole using high-performance liquid chromatography from nanostructured lipid carriers |
title_fullStr |
Validation of an innovative analytical method for simultaneous quantification of curcumin and fluconazole using high-performance liquid chromatography from nanostructured lipid carriers |
title_full_unstemmed |
Validation of an innovative analytical method for simultaneous quantification of curcumin and fluconazole using high-performance liquid chromatography from nanostructured lipid carriers |
title_sort |
Validation of an innovative analytical method for simultaneous quantification of curcumin and fluconazole using high-performance liquid chromatography from nanostructured lipid carriers |
author |
Araujo, Victor Hugo Sousa [UNESP] |
author_facet |
Araujo, Victor Hugo Sousa [UNESP] Fernandes, Lígia de Souza [UNESP] dos Reis, Leidiana Rocha [UNESP] Carvalho, Gabriela Corrêa [UNESP] Scarpa, Maria Virginia [UNESP] Chorilli, Marlus [UNESP] |
author_role |
author |
author2 |
Fernandes, Lígia de Souza [UNESP] dos Reis, Leidiana Rocha [UNESP] Carvalho, Gabriela Corrêa [UNESP] Scarpa, Maria Virginia [UNESP] Chorilli, Marlus [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Araujo, Victor Hugo Sousa [UNESP] Fernandes, Lígia de Souza [UNESP] dos Reis, Leidiana Rocha [UNESP] Carvalho, Gabriela Corrêa [UNESP] Scarpa, Maria Virginia [UNESP] Chorilli, Marlus [UNESP] |
dc.subject.por.fl_str_mv |
curcumin fluconazole nanostructured lipid carriers simultaneous detection |
topic |
curcumin fluconazole nanostructured lipid carriers simultaneous detection |
description |
Vulvovaginal candidiasis is a public health problem with a high incidence among female patients. Currently, there is an increase in the identification of Candida spp. resistant to current therapy, making it necessary to search for new therapeutic alternatives. The synergistic potential of curcumin with fluconazole is described in the literature. However, due to its high lipophilicity, it is necessary to use drug-delivery systems to adequately explore its potential, among which is the nanostructured lipid carrier. However, to date, there is no validated method of high-performance liquid chromatography for simultaneous determination of fluconazole and curcumin in the literature. Thus, the present work developed a high-performance liquid chromatography method for simultaneous determination of fluconazole and curcumin co-encapsulated in nanostructured lipid carrier which was validated according to the International Conference on Harmonization (Technical Requirements for Registration of Pharmaceuticals for Human Use) – Q2 (R1) and the Food and Drug Administration - Guidance for Bioanalytical Method. The method was applied to determine the encapsulation efficiency and drug-loading of curcumin and fluconazole in nanostructured lipid carriers. The developed method proved to be selective, precise, accurate, and robust for the simultaneous determination of both drugs, enabling the quantification of encapsulation efficiency and drug-loading of curcumin and fluconazole in nanostructured lipid carriers. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-01-01 2022-04-29T08:34:59Z 2022-04-29T08:34:59Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1002/jssc.202100514 Journal of Separation Science. 1615-9314 1615-9306 http://hdl.handle.net/11449/229658 10.1002/jssc.202100514 2-s2.0-85116518805 |
url |
http://dx.doi.org/10.1002/jssc.202100514 http://hdl.handle.net/11449/229658 |
identifier_str_mv |
Journal of Separation Science. 1615-9314 1615-9306 10.1002/jssc.202100514 2-s2.0-85116518805 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Separation Science |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799964894462738432 |