Adhesion of Candida biofilm cells to human epithelial cells and polystyrene after treatment with silver nanoparticles

Detalhes bibliográficos
Autor(a) principal: Monteiro, D. R.
Data de Publicação: 2014
Outros Autores: Negri, M., Silva, Sónia Carina, Gorup, L. F., Camargo, E. R., Oliveira, Rosário, Barbosa, D. B., Henriques, Mariana
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/31598
Resumo: This study investigated the adhesion to human epithelial cells and polystyrene surface of viable yeasts recovered from Candida biofilms treated with silver nanoparticles (SN). Biofilm resuspended Candida cells were added to HeLa cells or to empty wells of microtiter plates and the adhesion was verified using crystal violet staining. The adhesion of Candida cells was significantly reduced, mainly when biofilms were pretreated with 54 μg/mL SN. These new findings allow to conclude that SN may induce changes in viable yeasts, which can decrease the dissemination of Candida infections, mainly in susceptible patients.
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spelling Adhesion of Candida biofilm cells to human epithelial cells and polystyrene after treatment with silver nanoparticlesCandidaEpithelial cellsPolystyreneSilver nanoparticlesYeast adhesionScience & TechnologyThis study investigated the adhesion to human epithelial cells and polystyrene surface of viable yeasts recovered from Candida biofilms treated with silver nanoparticles (SN). Biofilm resuspended Candida cells were added to HeLa cells or to empty wells of microtiter plates and the adhesion was verified using crystal violet staining. The adhesion of Candida cells was significantly reduced, mainly when biofilms were pretreated with 54 μg/mL SN. These new findings allow to conclude that SN may induce changes in viable yeasts, which can decrease the dissemination of Candida infections, mainly in susceptible patients.We thank Dr. David Williams (Cardiff University, Cardiff, UK) for providing the strain 324LA/94, and George Duchow for the English review. Silver colloidal nanoparticles used herein were prepared by LIEC-CMDMC and INCTMN/FAPESP-CNPq, Sao Carlos, Brazil. This study was supported by CAPES (grant BEX 1221/10-8) and FAPESP (2009/15146-5), Brazil.Elsevier B.V.Universidade do MinhoMonteiro, D. R.Negri, M.Silva, Sónia CarinaGorup, L. F.Camargo, E. R.Oliveira, RosárioBarbosa, D. B.Henriques, Mariana20142014-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/31598engMonteiro, D. R.; Negri, M.; Silva, Sónia Carina; Gorup, L. F.; Camargo, E. R.; Oliveira, Rosário; Barbosa, D. B.; Henriques, Mariana, Adhesion of Candida biofilm cells to human epithelial cells and polystyrene after treatment with silver nanoparticles. Colloids and Surfaces B: Biointerfaces, 114, 410-412, 20140927-77650927-776510.1016/j.colsurfb.2013.10.02724257686info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:08:56Zoai:repositorium.sdum.uminho.pt:1822/31598Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:00:15.859980Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Adhesion of Candida biofilm cells to human epithelial cells and polystyrene after treatment with silver nanoparticles
title Adhesion of Candida biofilm cells to human epithelial cells and polystyrene after treatment with silver nanoparticles
spellingShingle Adhesion of Candida biofilm cells to human epithelial cells and polystyrene after treatment with silver nanoparticles
Monteiro, D. R.
Candida
Epithelial cells
Polystyrene
Silver nanoparticles
Yeast adhesion
Science & Technology
title_short Adhesion of Candida biofilm cells to human epithelial cells and polystyrene after treatment with silver nanoparticles
title_full Adhesion of Candida biofilm cells to human epithelial cells and polystyrene after treatment with silver nanoparticles
title_fullStr Adhesion of Candida biofilm cells to human epithelial cells and polystyrene after treatment with silver nanoparticles
title_full_unstemmed Adhesion of Candida biofilm cells to human epithelial cells and polystyrene after treatment with silver nanoparticles
title_sort Adhesion of Candida biofilm cells to human epithelial cells and polystyrene after treatment with silver nanoparticles
author Monteiro, D. R.
author_facet Monteiro, D. R.
Negri, M.
Silva, Sónia Carina
Gorup, L. F.
Camargo, E. R.
Oliveira, Rosário
Barbosa, D. B.
Henriques, Mariana
author_role author
author2 Negri, M.
Silva, Sónia Carina
Gorup, L. F.
Camargo, E. R.
Oliveira, Rosário
Barbosa, D. B.
Henriques, Mariana
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Monteiro, D. R.
Negri, M.
Silva, Sónia Carina
Gorup, L. F.
Camargo, E. R.
Oliveira, Rosário
Barbosa, D. B.
Henriques, Mariana
dc.subject.por.fl_str_mv Candida
Epithelial cells
Polystyrene
Silver nanoparticles
Yeast adhesion
Science & Technology
topic Candida
Epithelial cells
Polystyrene
Silver nanoparticles
Yeast adhesion
Science & Technology
description This study investigated the adhesion to human epithelial cells and polystyrene surface of viable yeasts recovered from Candida biofilms treated with silver nanoparticles (SN). Biofilm resuspended Candida cells were added to HeLa cells or to empty wells of microtiter plates and the adhesion was verified using crystal violet staining. The adhesion of Candida cells was significantly reduced, mainly when biofilms were pretreated with 54 μg/mL SN. These new findings allow to conclude that SN may induce changes in viable yeasts, which can decrease the dissemination of Candida infections, mainly in susceptible patients.
publishDate 2014
dc.date.none.fl_str_mv 2014
2014-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/31598
url http://hdl.handle.net/1822/31598
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Monteiro, D. R.; Negri, M.; Silva, Sónia Carina; Gorup, L. F.; Camargo, E. R.; Oliveira, Rosário; Barbosa, D. B.; Henriques, Mariana, Adhesion of Candida biofilm cells to human epithelial cells and polystyrene after treatment with silver nanoparticles. Colloids and Surfaces B: Biointerfaces, 114, 410-412, 2014
0927-7765
0927-7765
10.1016/j.colsurfb.2013.10.027
24257686
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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