Fluoxetine arrests growth of the model diatom Phaeodactylum tricornutum by increasing oxidative stress and altering energetic and lipid metabolism
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2020 |
| Outros Autores: | , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/10400.8/6076 |
Resumo: | FUNDING : The authors would like to thank Fundação para a Ciência e a Tecnologia (FCT) for funding the research via project grants PTDC/MAR-EST/3048/2014 (BIOPHARMA), PTDC/CTA-AMB/30056/2017 (OPTOX), UIDB/04292/2020, and UID/MULTI/04046/2013. Work was also funded by the Integrated Programme of SR&TD SmartBioR (reference Centro-01-0145-FEDER-000018), co-funded by Centro 2020 program, Portugal 2020, European Union, through the European Regional Development Fund. BD and VF were supported by investigation contracts (CEECIND/00511/2017 and DL57/2016/CP1479/CT0024). PR-S was supported by FCT through a postdoctoral grant (SFRH/BPD/95784/2013). ID was supported by a Ph.D. grant (SFRH/BD/138376/2018). MC acknowledges the FCT and IGOT (contract under the DL 57/2016 and L57/2017 Program). |
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Fluoxetine arrests growth of the model diatom Phaeodactylum tricornutum by increasing oxidative stress and altering energetic and lipid metabolismPharmaceuticalsAntidepressantMicroalgaeEcotoxicityPhotobiologyCell energyBiomarkersFatty acid profileFUNDING : The authors would like to thank Fundação para a Ciência e a Tecnologia (FCT) for funding the research via project grants PTDC/MAR-EST/3048/2014 (BIOPHARMA), PTDC/CTA-AMB/30056/2017 (OPTOX), UIDB/04292/2020, and UID/MULTI/04046/2013. Work was also funded by the Integrated Programme of SR&TD SmartBioR (reference Centro-01-0145-FEDER-000018), co-funded by Centro 2020 program, Portugal 2020, European Union, through the European Regional Development Fund. BD and VF were supported by investigation contracts (CEECIND/00511/2017 and DL57/2016/CP1479/CT0024). PR-S was supported by FCT through a postdoctoral grant (SFRH/BPD/95784/2013). ID was supported by a Ph.D. grant (SFRH/BD/138376/2018). MC acknowledges the FCT and IGOT (contract under the DL 57/2016 and L57/2017 Program).Pharmaceutical residues impose a new and emerging threat to aquatic environments and its biota. One of the most commonly prescribed pharmaceuticals is the antidepressant fluoxetine, a selective serotonin re-uptake inhibitor that has been frequently detected, in concentrations up to 40 ug L-1, in aquatic ecosystems. The present study aims to investigate the ecotoxicity of fluoxetine at environmentally relevant concentrations (0.3, 0.6, 20, 40, and 80 ug L-1) on cell energy and lipid metabolism, as well as oxidative stress biomarkers in the model diatom Phaeodactylum tricornutum. Exposure to higher concentrations of fluoxetine negatively affected cell density and photosynthesis through a decrease in the active PSII reaction centers. Stress response mechanisms, like b-carotene (b-car) production and antioxidant enzymes [superoxide dismutase (SOD) and ascorbate peroxidase (APX)] up-regulation were triggered, likely as a positive feedback mechanism toward formation of fluoxetine-induced reactive oxygen species. Lipid peroxidation products increased greatly at the highest fluoxetine concentration whereas no variation in the relative amounts of long chain polyunsaturated fatty acids (LC-PUFAs) was observed. However, monogalactosyldiacylglycerol-characteristic fatty acids such as C16:2 and C16:3 increased, suggesting an interaction between light harvesting pigments, lipid environment, and photosynthesis stabilization. Using a canonical multivariate analysis, it was possible to evaluate the efficiency of the application of bio-optical and biochemical techniques as potential fluoxetine exposure biomarkers in P. tricornutum. An overall classification efficiency to the different levels of fluoxetine exposure of 61.1 and 88.9% were obtained for bio-optical and fatty acids profiles, respectively, with different resolution degrees highlighting these parameters as potential efficient biomarkers. Additionally, the negative impact of this pharmaceutical molecule on the primary productivity is also evident alongside with an increase in respiratory oxygen consumption. From the ecological point of view, reduction in diatom biomass due to continued exposure to fluoxetine may severely impact estuarine and coastal trophic webs, by both a reduction in oxygen primary productivity and reduced availability of key fatty acids to the dependent heterotrophic upper levels.FrontiersIC-OnlineFeijão, EduardoCarvalho, Ricardo Cruz deDuarte, Irina A.Matos, Ana RitaCabrita, Maria TeresaNovais, Sara C.Lemos, Marco F.L.Caçador, IsabelMarques, João CarlosReis-Santos, PatrickFonseca, Vanessa F.Duarte, Bernardo2021-08-11T12:05:56Z20202020-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.8/6076engFeijão E, Cruz de Carvalho R, Duarte IA, Matos AR, Cabrita MT, Novais SC, Lemos MFL, Caçador I, Marques JC, Reis-Santos P, Fonseca VF and Duarte B (2020) Fluoxetine Arrests Growth of the Model Diatom Phaeodactylum tricornutum by Increasing Oxidative Stress and Altering Energetic and Lipid Metabolism. Front. Microbiol. 11:1803. doi: 10.3389/fmicb.2020.018031664-302X10.3389/fmicb.2020.01803info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-17T15:52:20Zoai:iconline.ipleiria.pt:10400.8/6076Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:49:24.560311Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
Fluoxetine arrests growth of the model diatom Phaeodactylum tricornutum by increasing oxidative stress and altering energetic and lipid metabolism |
| title |
Fluoxetine arrests growth of the model diatom Phaeodactylum tricornutum by increasing oxidative stress and altering energetic and lipid metabolism |
| spellingShingle |
Fluoxetine arrests growth of the model diatom Phaeodactylum tricornutum by increasing oxidative stress and altering energetic and lipid metabolism Feijão, Eduardo Pharmaceuticals Antidepressant Microalgae Ecotoxicity Photobiology Cell energy Biomarkers Fatty acid profile |
| title_short |
Fluoxetine arrests growth of the model diatom Phaeodactylum tricornutum by increasing oxidative stress and altering energetic and lipid metabolism |
| title_full |
Fluoxetine arrests growth of the model diatom Phaeodactylum tricornutum by increasing oxidative stress and altering energetic and lipid metabolism |
| title_fullStr |
Fluoxetine arrests growth of the model diatom Phaeodactylum tricornutum by increasing oxidative stress and altering energetic and lipid metabolism |
| title_full_unstemmed |
Fluoxetine arrests growth of the model diatom Phaeodactylum tricornutum by increasing oxidative stress and altering energetic and lipid metabolism |
| title_sort |
Fluoxetine arrests growth of the model diatom Phaeodactylum tricornutum by increasing oxidative stress and altering energetic and lipid metabolism |
| author |
Feijão, Eduardo |
| author_facet |
Feijão, Eduardo Carvalho, Ricardo Cruz de Duarte, Irina A. Matos, Ana Rita Cabrita, Maria Teresa Novais, Sara C. Lemos, Marco F.L. Caçador, Isabel Marques, João Carlos Reis-Santos, Patrick Fonseca, Vanessa F. Duarte, Bernardo |
| author_role |
author |
| author2 |
Carvalho, Ricardo Cruz de Duarte, Irina A. Matos, Ana Rita Cabrita, Maria Teresa Novais, Sara C. Lemos, Marco F.L. Caçador, Isabel Marques, João Carlos Reis-Santos, Patrick Fonseca, Vanessa F. Duarte, Bernardo |
| author2_role |
author author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
IC-Online |
| dc.contributor.author.fl_str_mv |
Feijão, Eduardo Carvalho, Ricardo Cruz de Duarte, Irina A. Matos, Ana Rita Cabrita, Maria Teresa Novais, Sara C. Lemos, Marco F.L. Caçador, Isabel Marques, João Carlos Reis-Santos, Patrick Fonseca, Vanessa F. Duarte, Bernardo |
| dc.subject.por.fl_str_mv |
Pharmaceuticals Antidepressant Microalgae Ecotoxicity Photobiology Cell energy Biomarkers Fatty acid profile |
| topic |
Pharmaceuticals Antidepressant Microalgae Ecotoxicity Photobiology Cell energy Biomarkers Fatty acid profile |
| description |
FUNDING : The authors would like to thank Fundação para a Ciência e a Tecnologia (FCT) for funding the research via project grants PTDC/MAR-EST/3048/2014 (BIOPHARMA), PTDC/CTA-AMB/30056/2017 (OPTOX), UIDB/04292/2020, and UID/MULTI/04046/2013. Work was also funded by the Integrated Programme of SR&TD SmartBioR (reference Centro-01-0145-FEDER-000018), co-funded by Centro 2020 program, Portugal 2020, European Union, through the European Regional Development Fund. BD and VF were supported by investigation contracts (CEECIND/00511/2017 and DL57/2016/CP1479/CT0024). PR-S was supported by FCT through a postdoctoral grant (SFRH/BPD/95784/2013). ID was supported by a Ph.D. grant (SFRH/BD/138376/2018). MC acknowledges the FCT and IGOT (contract under the DL 57/2016 and L57/2017 Program). |
| publishDate |
2020 |
| dc.date.none.fl_str_mv |
2020 2020-01-01T00:00:00Z 2021-08-11T12:05:56Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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http://hdl.handle.net/10400.8/6076 |
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http://hdl.handle.net/10400.8/6076 |
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eng |
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eng |
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Feijão E, Cruz de Carvalho R, Duarte IA, Matos AR, Cabrita MT, Novais SC, Lemos MFL, Caçador I, Marques JC, Reis-Santos P, Fonseca VF and Duarte B (2020) Fluoxetine Arrests Growth of the Model Diatom Phaeodactylum tricornutum by Increasing Oxidative Stress and Altering Energetic and Lipid Metabolism. Front. Microbiol. 11:1803. doi: 10.3389/fmicb.2020.01803 1664-302X 10.3389/fmicb.2020.01803 |
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Frontiers |
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Frontiers |
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