Early Identification of Plant Drought Stress Responses: Changes in Leaf Reflectance and Plant Growth Promoting Rhizobacteria Selection-The Case Study of Tomato Plants
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10451/59780 |
Resumo: | Drought is a worldwide problem, especially in arid and semi-arid regions. Detection of drought stress at the initial stages, before visible signs, to adequately manage irrigation and crop fertilization to avoid crop yield loss, is a desire of most farmers. Here, we evaluated the response of tomato plants to water scarcity, through changes in leaf reflectance due to modification in leaf pigments’ content, which translates into differences in spectral reflectance indices (SRI) values. Our methodology is innovative, as we were able to easily calculate and identify several SRIs for the early detection of drought stress “invisible” responses. We used a handheld spectro-radiometer to obtain SRI values from leaves of tomato plants growing under two different water regimes for 37 days. In an ensemble of 25 SRIs, we identified 12 that showed a consistent trend of significant differences between treatments along the experiment and within these, NDVI, SR, ZMI, Ctr2, GM1, and GM2 were already significantly different between treatments at day 7 after the start of the experiment and Ctr1 at day 9; although, no signs of damage were visible. Therefore, our results pinpoint these SRIs as promising proxies for the early detection of “invisible” responses to drought onset. We also analyzed the relationship between the monitored SRIs and plant morphological parameters measured during the experiment, highlighting a relationship between GM1 and plant height and leaf number. Finally, we observed a high abundance of putative beneficial bacteria among isolates collected from the tomato water-limited rhizo-environment at the terminus of the experiment, suggesting the active recruitment or selection of Plant Growth Promoting Rhizobacteria by tomato roots as a response to drought. Our work may be adapted into an easy protocol, of rapid execution, to be used in small-scale fields for early drought stress detection. |
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Early Identification of Plant Drought Stress Responses: Changes in Leaf Reflectance and Plant Growth Promoting Rhizobacteria Selection-The Case Study of Tomato PlantsDrought is a worldwide problem, especially in arid and semi-arid regions. Detection of drought stress at the initial stages, before visible signs, to adequately manage irrigation and crop fertilization to avoid crop yield loss, is a desire of most farmers. Here, we evaluated the response of tomato plants to water scarcity, through changes in leaf reflectance due to modification in leaf pigments’ content, which translates into differences in spectral reflectance indices (SRI) values. Our methodology is innovative, as we were able to easily calculate and identify several SRIs for the early detection of drought stress “invisible” responses. We used a handheld spectro-radiometer to obtain SRI values from leaves of tomato plants growing under two different water regimes for 37 days. In an ensemble of 25 SRIs, we identified 12 that showed a consistent trend of significant differences between treatments along the experiment and within these, NDVI, SR, ZMI, Ctr2, GM1, and GM2 were already significantly different between treatments at day 7 after the start of the experiment and Ctr1 at day 9; although, no signs of damage were visible. Therefore, our results pinpoint these SRIs as promising proxies for the early detection of “invisible” responses to drought onset. We also analyzed the relationship between the monitored SRIs and plant morphological parameters measured during the experiment, highlighting a relationship between GM1 and plant height and leaf number. Finally, we observed a high abundance of putative beneficial bacteria among isolates collected from the tomato water-limited rhizo-environment at the terminus of the experiment, suggesting the active recruitment or selection of Plant Growth Promoting Rhizobacteria by tomato roots as a response to drought. Our work may be adapted into an easy protocol, of rapid execution, to be used in small-scale fields for early drought stress detection.MDPIRepositório da Universidade de LisboaRosa, Ana PaulaBarão, LúciaChambel, LéliaCruz, CristinaSantana, Margarida2023-10-16T11:56:45Z2023-012023-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/59780engRosa, A.P.; Barão, L.; Chambel, L.; Cruz, C.; Santana, M.M. Early Identification of Plant Drought Stress Responses: Changes in Leaf Reflectance and Plant Growth Promoting Rhizobacteria Selection-The Case Study of Tomato Plants. Agronomy 2023, 13, 183. https://doi.org/10.3390/agronomy1301018310.3390/agronomy13010183info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T17:09:25Zoai:repositorio.ul.pt:10451/59780Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T22:09:45.566439Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Early Identification of Plant Drought Stress Responses: Changes in Leaf Reflectance and Plant Growth Promoting Rhizobacteria Selection-The Case Study of Tomato Plants |
title |
Early Identification of Plant Drought Stress Responses: Changes in Leaf Reflectance and Plant Growth Promoting Rhizobacteria Selection-The Case Study of Tomato Plants |
spellingShingle |
Early Identification of Plant Drought Stress Responses: Changes in Leaf Reflectance and Plant Growth Promoting Rhizobacteria Selection-The Case Study of Tomato Plants Rosa, Ana Paula |
title_short |
Early Identification of Plant Drought Stress Responses: Changes in Leaf Reflectance and Plant Growth Promoting Rhizobacteria Selection-The Case Study of Tomato Plants |
title_full |
Early Identification of Plant Drought Stress Responses: Changes in Leaf Reflectance and Plant Growth Promoting Rhizobacteria Selection-The Case Study of Tomato Plants |
title_fullStr |
Early Identification of Plant Drought Stress Responses: Changes in Leaf Reflectance and Plant Growth Promoting Rhizobacteria Selection-The Case Study of Tomato Plants |
title_full_unstemmed |
Early Identification of Plant Drought Stress Responses: Changes in Leaf Reflectance and Plant Growth Promoting Rhizobacteria Selection-The Case Study of Tomato Plants |
title_sort |
Early Identification of Plant Drought Stress Responses: Changes in Leaf Reflectance and Plant Growth Promoting Rhizobacteria Selection-The Case Study of Tomato Plants |
author |
Rosa, Ana Paula |
author_facet |
Rosa, Ana Paula Barão, Lúcia Chambel, Lélia Cruz, Cristina Santana, Margarida |
author_role |
author |
author2 |
Barão, Lúcia Chambel, Lélia Cruz, Cristina Santana, Margarida |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Repositório da Universidade de Lisboa |
dc.contributor.author.fl_str_mv |
Rosa, Ana Paula Barão, Lúcia Chambel, Lélia Cruz, Cristina Santana, Margarida |
description |
Drought is a worldwide problem, especially in arid and semi-arid regions. Detection of drought stress at the initial stages, before visible signs, to adequately manage irrigation and crop fertilization to avoid crop yield loss, is a desire of most farmers. Here, we evaluated the response of tomato plants to water scarcity, through changes in leaf reflectance due to modification in leaf pigments’ content, which translates into differences in spectral reflectance indices (SRI) values. Our methodology is innovative, as we were able to easily calculate and identify several SRIs for the early detection of drought stress “invisible” responses. We used a handheld spectro-radiometer to obtain SRI values from leaves of tomato plants growing under two different water regimes for 37 days. In an ensemble of 25 SRIs, we identified 12 that showed a consistent trend of significant differences between treatments along the experiment and within these, NDVI, SR, ZMI, Ctr2, GM1, and GM2 were already significantly different between treatments at day 7 after the start of the experiment and Ctr1 at day 9; although, no signs of damage were visible. Therefore, our results pinpoint these SRIs as promising proxies for the early detection of “invisible” responses to drought onset. We also analyzed the relationship between the monitored SRIs and plant morphological parameters measured during the experiment, highlighting a relationship between GM1 and plant height and leaf number. Finally, we observed a high abundance of putative beneficial bacteria among isolates collected from the tomato water-limited rhizo-environment at the terminus of the experiment, suggesting the active recruitment or selection of Plant Growth Promoting Rhizobacteria by tomato roots as a response to drought. Our work may be adapted into an easy protocol, of rapid execution, to be used in small-scale fields for early drought stress detection. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-10-16T11:56:45Z 2023-01 2023-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10451/59780 |
url |
http://hdl.handle.net/10451/59780 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Rosa, A.P.; Barão, L.; Chambel, L.; Cruz, C.; Santana, M.M. Early Identification of Plant Drought Stress Responses: Changes in Leaf Reflectance and Plant Growth Promoting Rhizobacteria Selection-The Case Study of Tomato Plants. Agronomy 2023, 13, 183. https://doi.org/10.3390/agronomy13010183 10.3390/agronomy13010183 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
MDPI |
publisher.none.fl_str_mv |
MDPI |
dc.source.none.fl_str_mv |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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