Selection and characterization of a multivalent Salmonella phage and its production in a nonpathogenic Escherichia coli strain

Detalhes bibliográficos
Autor(a) principal: Santos, Sílvio Roberto Branco
Data de Publicação: 2010
Outros Autores: Fernandes, Elisabete Ramos, Carvalho, Carla M., Sillankorva, Sanna, Krylov, V. N., Pleteneva, E. A., Shaburova, O. V., Nicolau, Ana, Ferreira, Eugénio C., Azeredo, Joana
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://hdl.handle.net/1822/11462
Resumo: We report the selection and amplification of the broad-host-range Salmonella phage phi PVP-SE1 in an alternative nonpathogenic host. The lytic spectrum and the phage DNA restriction profile were not modified upon replication in Escherichia coli Bl21, suggesting the possibility of producing this phage in a nonpathogenic host, contributing to the safety and easier approval of a product based on this Salmonella biocontrol agent.
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spelling Selection and characterization of a multivalent Salmonella phage and its production in a nonpathogenic Escherichia coli strainScience & TechnologyWe report the selection and amplification of the broad-host-range Salmonella phage phi PVP-SE1 in an alternative nonpathogenic host. The lytic spectrum and the phage DNA restriction profile were not modified upon replication in Escherichia coli Bl21, suggesting the possibility of producing this phage in a nonpathogenic host, contributing to the safety and easier approval of a product based on this Salmonella biocontrol agent.Fundação para a Ciência e a Tecnologia (FCT)American Society for Microbiology (ASM)Universidade do MinhoSantos, Sílvio Roberto BrancoFernandes, Elisabete RamosCarvalho, Carla M.Sillankorva, SannaKrylov, V. N.Pleteneva, E. A.Shaburova, O. V.Nicolau, AnaFerreira, Eugénio C.Azeredo, Joana2010-112010-11-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/11462eng"Applied and Environmental Microbiology". ISSN 0099-2240. 76 :21 (Nov. 2010) 7338-7342-0099-224010.1128/AEM.00922-1020817806info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:47:59Zoai:repositorium.sdum.uminho.pt:1822/11462Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:46:08.382393Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Selection and characterization of a multivalent Salmonella phage and its production in a nonpathogenic Escherichia coli strain
title Selection and characterization of a multivalent Salmonella phage and its production in a nonpathogenic Escherichia coli strain
spellingShingle Selection and characterization of a multivalent Salmonella phage and its production in a nonpathogenic Escherichia coli strain
Santos, Sílvio Roberto Branco
Science & Technology
title_short Selection and characterization of a multivalent Salmonella phage and its production in a nonpathogenic Escherichia coli strain
title_full Selection and characterization of a multivalent Salmonella phage and its production in a nonpathogenic Escherichia coli strain
title_fullStr Selection and characterization of a multivalent Salmonella phage and its production in a nonpathogenic Escherichia coli strain
title_full_unstemmed Selection and characterization of a multivalent Salmonella phage and its production in a nonpathogenic Escherichia coli strain
title_sort Selection and characterization of a multivalent Salmonella phage and its production in a nonpathogenic Escherichia coli strain
author Santos, Sílvio Roberto Branco
author_facet Santos, Sílvio Roberto Branco
Fernandes, Elisabete Ramos
Carvalho, Carla M.
Sillankorva, Sanna
Krylov, V. N.
Pleteneva, E. A.
Shaburova, O. V.
Nicolau, Ana
Ferreira, Eugénio C.
Azeredo, Joana
author_role author
author2 Fernandes, Elisabete Ramos
Carvalho, Carla M.
Sillankorva, Sanna
Krylov, V. N.
Pleteneva, E. A.
Shaburova, O. V.
Nicolau, Ana
Ferreira, Eugénio C.
Azeredo, Joana
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Santos, Sílvio Roberto Branco
Fernandes, Elisabete Ramos
Carvalho, Carla M.
Sillankorva, Sanna
Krylov, V. N.
Pleteneva, E. A.
Shaburova, O. V.
Nicolau, Ana
Ferreira, Eugénio C.
Azeredo, Joana
dc.subject.por.fl_str_mv Science & Technology
topic Science & Technology
description We report the selection and amplification of the broad-host-range Salmonella phage phi PVP-SE1 in an alternative nonpathogenic host. The lytic spectrum and the phage DNA restriction profile were not modified upon replication in Escherichia coli Bl21, suggesting the possibility of producing this phage in a nonpathogenic host, contributing to the safety and easier approval of a product based on this Salmonella biocontrol agent.
publishDate 2010
dc.date.none.fl_str_mv 2010-11
2010-11-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://hdl.handle.net/1822/11462
url https://hdl.handle.net/1822/11462
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv "Applied and Environmental Microbiology". ISSN 0099-2240. 76 :21 (Nov. 2010) 7338-7342-
0099-2240
10.1128/AEM.00922-10
20817806
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv American Society for Microbiology (ASM)
publisher.none.fl_str_mv American Society for Microbiology (ASM)
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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