Denervation impairs regeneration of amputated Zebrafish fins

Detalhes bibliográficos
Autor(a) principal: Simões, Mariana G
Data de Publicação: 2014
Outros Autores: Bensimon-Brito, Anabela, Fonseca, Mariana, Farinho, Ana, Valério, Fábio, Sousa, Sara, Afonso, Nuno, Kumar, Anoop, Jacinto, Antonio
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/51867
Resumo: © 2014 Simões et al.; licensee BioMed Central. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
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spelling Denervation impairs regeneration of amputated Zebrafish fins© 2014 Simões et al.; licensee BioMed Central. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.Background: Zebrafish are able to regenerate many of its tissues and organs after damage. In amphibians this process is regulated by nerve fibres present at the site of injury, which have been proposed to release factors into the amputated limbs/fins, promoting and sustaining the proliferation of blastemal cells. Although some candidate factors have been proposed to mediate the nerve dependency of regeneration, the molecular mechanisms involved in this process remain unclear. Results: We have used zebrafish as a model system to address the role of nerve fibres in fin regeneration. We have developed a protocol for pectoral fin denervation followed by amputation and analysed the regenerative process under this experimental conditions. Upon denervation fins were able to close the wound and form a wound epidermis, but could not establish a functional apical epithelial cap, with a posterior failure of blastema formation and outgrowth, and the accumulation of several defects. The expression patterns of genes known to be key players during fin regeneration were altered upon denervation, suggesting that nerves can contribute to the regulation of the Fgf, Wnt and Shh pathways during zebrafish fin regeneration. Conclusions: Our results demonstrate that proper innervation of the zebrafish pectoral fin is essential for a successful regenerative process, and establish this organism as a useful model to understand the molecular and cellular mechanisms of nerve dependence, during vertebrate regeneration.ariana Guedes Simões was supported by a PhD grant from Fundação para a Ciência e Tecnologia (FCT), Portugal. Anabela Bensimon-Brito was suported by the ERC Reseal project (2007-StG-208631). The research presented in this manuscript has been funded by FCT research grants (PTDC/SAU-GMG/101537/2008 PTDC/SAU- OBD/100200/ 2008 PTDC/BEX-BID/1176/2012).Springer NatureRepositório da Universidade de LisboaSimões, Mariana GBensimon-Brito, AnabelaFonseca, MarianaFarinho, AnaValério, FábioSousa, SaraAfonso, NunoKumar, AnoopJacinto, Antonio2022-03-21T17:24:46Z20142014-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/51867engBMC Dev Biol. 2014 Dec 31;14:4910.1186/s12861-014-0049-21471-213Xinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T16:56:51Zoai:repositorio.ul.pt:10451/51867Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T22:03:05.463611Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Denervation impairs regeneration of amputated Zebrafish fins
title Denervation impairs regeneration of amputated Zebrafish fins
spellingShingle Denervation impairs regeneration of amputated Zebrafish fins
Simões, Mariana G
title_short Denervation impairs regeneration of amputated Zebrafish fins
title_full Denervation impairs regeneration of amputated Zebrafish fins
title_fullStr Denervation impairs regeneration of amputated Zebrafish fins
title_full_unstemmed Denervation impairs regeneration of amputated Zebrafish fins
title_sort Denervation impairs regeneration of amputated Zebrafish fins
author Simões, Mariana G
author_facet Simões, Mariana G
Bensimon-Brito, Anabela
Fonseca, Mariana
Farinho, Ana
Valério, Fábio
Sousa, Sara
Afonso, Nuno
Kumar, Anoop
Jacinto, Antonio
author_role author
author2 Bensimon-Brito, Anabela
Fonseca, Mariana
Farinho, Ana
Valério, Fábio
Sousa, Sara
Afonso, Nuno
Kumar, Anoop
Jacinto, Antonio
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Simões, Mariana G
Bensimon-Brito, Anabela
Fonseca, Mariana
Farinho, Ana
Valério, Fábio
Sousa, Sara
Afonso, Nuno
Kumar, Anoop
Jacinto, Antonio
description © 2014 Simões et al.; licensee BioMed Central. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
publishDate 2014
dc.date.none.fl_str_mv 2014
2014-01-01T00:00:00Z
2022-03-21T17:24:46Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/51867
url http://hdl.handle.net/10451/51867
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv BMC Dev Biol. 2014 Dec 31;14:49
10.1186/s12861-014-0049-2
1471-213X
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
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dc.publisher.none.fl_str_mv Springer Nature
publisher.none.fl_str_mv Springer Nature
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repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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