codY and pdhA expression is induced in Staphylococcus epidermidis biofilm and planktonic populations With higher proportions of viable but non-culturable cells

Detalhes bibliográficos
Autor(a) principal: Gaio, Vania
Data de Publicação: 2021
Outros Autores: Lopes, Nathalie, Cerca, Nuno, França, Ângela Maria Oliveira Sousa
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/75264
Resumo: The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fcimb.2021.771666/full#supplementary-material
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spelling codY and pdhA expression is induced in Staphylococcus epidermidis biofilm and planktonic populations With higher proportions of viable but non-culturable cellsStaphylococcus epidermidis infectionsVBNC cells induction modelplanktonic cellsbiofilmsglucosemagnesiumgene expressionScience & TechnologyThe Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fcimb.2021.771666/full#supplementary-materialStaphylococcus epidermidis biofilm cells can enter a physiological state known as viable but non-culturable (VBNC), where, despite being alive, they do not grow in conventional laboratory media. As such, the presence of VBNC cells impacts the diagnosis of S. epidermidis biofilm-associated infections. Previous transcriptomics analysis of S. epidermidis strain 9142 biofilms with higher proportions of VBNC cells suggested that the genes pdhA, codY and mazEF could be involved in the induction of the VBNC state. However, it was previously demonstrated that VBNC induction is strain-dependent. To properly assess the role of these genes in VBNC induction, the construction of mutant strains is necessary. Thus, herein, we assessed if VBNC cells could be induced in strain 1457, a strain amenable to genetic manipulation, and if the previously identified genes were involved in the modulation of the VBNC state in this strain. Furthermore, we evaluated the formation of VBNC cells on planktonic cultures. Our results showed that despite being commonly associated with biofilms, the proportion of VBNC cells can be modulated in both biofilm and planktonic cultures and that the expression of codY and pdhA was upregulated under VBNC inducing conditions in both phenotypes. Overall, our study revealed that the formation of VBNC cells in S. epidermidis is independent of the mode of growth and that the genes codY and pdhA seem to be relevant for the regulation of this physiological condition.This work was supported by the Portuguese Foundation for Science and Technology (FCT) by the funded project PTDC/BIA-MOL/29553/2017, under the scope of COMPETE2020 (POCI-01-0145-FEDER-029553) and by the strategic funding of unit UIDB/04469/2020. VG and NL acknowledge the support of FCT individual fellowships [SFRH/BD/131452/2017 and SFRH/BD/136998/2018], respectively.info:eu-repo/semantics/publishedVersionFrontiers Media S. A.Universidade do MinhoGaio, VaniaLopes, NathalieCerca, NunoFrança, Ângela Maria Oliveira Sousa2021-11-152021-11-15T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/75264engGaio, Vania; Lopes, Nathalie; Cerca, Nuno; França, Angela, codY and pdhA expression is induced in Staphylococcus epidermidis biofilm and planktonic populations With higher proportions of viable but non-culturable cells. Frontiers in Cellular and Infection Microbiology, 11(771666), 20212235-298810.3389/fcimb.2021.77166634869073771666https://www.frontiersin.org/articles/10.3389/fcimb.2021.771666/fullinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T11:58:45Zoai:repositorium.sdum.uminho.pt:1822/75264Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:48:31.252753Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv codY and pdhA expression is induced in Staphylococcus epidermidis biofilm and planktonic populations With higher proportions of viable but non-culturable cells
title codY and pdhA expression is induced in Staphylococcus epidermidis biofilm and planktonic populations With higher proportions of viable but non-culturable cells
spellingShingle codY and pdhA expression is induced in Staphylococcus epidermidis biofilm and planktonic populations With higher proportions of viable but non-culturable cells
Gaio, Vania
Staphylococcus epidermidis infections
VBNC cells induction model
planktonic cells
biofilms
glucose
magnesium
gene expression
Science & Technology
title_short codY and pdhA expression is induced in Staphylococcus epidermidis biofilm and planktonic populations With higher proportions of viable but non-culturable cells
title_full codY and pdhA expression is induced in Staphylococcus epidermidis biofilm and planktonic populations With higher proportions of viable but non-culturable cells
title_fullStr codY and pdhA expression is induced in Staphylococcus epidermidis biofilm and planktonic populations With higher proportions of viable but non-culturable cells
title_full_unstemmed codY and pdhA expression is induced in Staphylococcus epidermidis biofilm and planktonic populations With higher proportions of viable but non-culturable cells
title_sort codY and pdhA expression is induced in Staphylococcus epidermidis biofilm and planktonic populations With higher proportions of viable but non-culturable cells
author Gaio, Vania
author_facet Gaio, Vania
Lopes, Nathalie
Cerca, Nuno
França, Ângela Maria Oliveira Sousa
author_role author
author2 Lopes, Nathalie
Cerca, Nuno
França, Ângela Maria Oliveira Sousa
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Gaio, Vania
Lopes, Nathalie
Cerca, Nuno
França, Ângela Maria Oliveira Sousa
dc.subject.por.fl_str_mv Staphylococcus epidermidis infections
VBNC cells induction model
planktonic cells
biofilms
glucose
magnesium
gene expression
Science & Technology
topic Staphylococcus epidermidis infections
VBNC cells induction model
planktonic cells
biofilms
glucose
magnesium
gene expression
Science & Technology
description The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fcimb.2021.771666/full#supplementary-material
publishDate 2021
dc.date.none.fl_str_mv 2021-11-15
2021-11-15T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/75264
url http://hdl.handle.net/1822/75264
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Gaio, Vania; Lopes, Nathalie; Cerca, Nuno; França, Angela, codY and pdhA expression is induced in Staphylococcus epidermidis biofilm and planktonic populations With higher proportions of viable but non-culturable cells. Frontiers in Cellular and Infection Microbiology, 11(771666), 2021
2235-2988
10.3389/fcimb.2021.771666
34869073
771666
https://www.frontiersin.org/articles/10.3389/fcimb.2021.771666/full
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Frontiers Media S. A.
publisher.none.fl_str_mv Frontiers Media S. A.
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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