Gold nanoparticle to antibody conjugates for diagnosis applications: molecular interactions and immunoassay development

Detalhes bibliográficos
Autor(a) principal: Cavadas, Miguel Alexandre dos Santos
Data de Publicação: 2011
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/6673
Resumo: Thesis for the Master degree in Structural and Functional Biochemistry
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spelling Gold nanoparticle to antibody conjugates for diagnosis applications: molecular interactions and immunoassay developmentGold nanoparticlesAntibodyAntigenBionanoconjugatesDiagnosisThesis for the Master degree in Structural and Functional BiochemistryBionanotechnology is an area of rapidly increasing research interest, whose development can potentially improve many fields of our society by providing several advances in biomedical, energy and environment research fields. The present work includes both applied and basic research on bionanoconjugates of gold nanoparticles (AuNP) with antibodies (Ab) referred to as AuNP@Ab. The two main themes of research are: i) development of a fluorescence immunoassay for malaria diagnosis, using a sensing methodology involving the competitive binding between a fluorophore-labeled antigen (CyAg) and the unlabeled antigen (Ag) to the AuNP@Ab conjugates. The Ag is a new biomarker of malaria infection, the heat shock protein 70 from Plasmodium falciparum (PfHsp70). ii) physico-chemical characterization of the interaction between the Ag and the AuNP@Ab using Fluorescence, Differential Centrifugal Sedimentation (DCS), and Agarose Gel Electrophoresis(AGE), intended at understanding the nature of the binding, particularly its specificity and the influence of nonspecific protein competitors. The immunoassay for malaria diagnosis was successfully established using fluorescence detection for AuNPs of different diameters (15 and 30 nm) and using AGE detection for 30 nm-AuNPs. The AuNP@Ab bionanoconjugates exhibited a specific response to the target Ag, with little, or none, non-specific binding when purified transferrin (Trf) is used. Non-specific binding of proteins from plasma was nevertheless detected. Ag binding to AuNP@Ab was assessed in competitive conditions both with plasma and with Trf. Trf inhibited the Ag binding, while plasma inhibited the Ag binding as accessed by DCS but the same conclusion could not be made for fluorescence due to the large background signal caused by plasma unspecific binding.Faculdade de Ciências e TecnologiaFranco, RicardoRUNCavadas, Miguel Alexandre dos Santos2012-01-11T12:08:34Z20112011-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10362/6673enginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T03:37:52Zoai:run.unl.pt:10362/6673Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:16:56.162142Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Gold nanoparticle to antibody conjugates for diagnosis applications: molecular interactions and immunoassay development
title Gold nanoparticle to antibody conjugates for diagnosis applications: molecular interactions and immunoassay development
spellingShingle Gold nanoparticle to antibody conjugates for diagnosis applications: molecular interactions and immunoassay development
Cavadas, Miguel Alexandre dos Santos
Gold nanoparticles
Antibody
Antigen
Bionanoconjugates
Diagnosis
title_short Gold nanoparticle to antibody conjugates for diagnosis applications: molecular interactions and immunoassay development
title_full Gold nanoparticle to antibody conjugates for diagnosis applications: molecular interactions and immunoassay development
title_fullStr Gold nanoparticle to antibody conjugates for diagnosis applications: molecular interactions and immunoassay development
title_full_unstemmed Gold nanoparticle to antibody conjugates for diagnosis applications: molecular interactions and immunoassay development
title_sort Gold nanoparticle to antibody conjugates for diagnosis applications: molecular interactions and immunoassay development
author Cavadas, Miguel Alexandre dos Santos
author_facet Cavadas, Miguel Alexandre dos Santos
author_role author
dc.contributor.none.fl_str_mv Franco, Ricardo
RUN
dc.contributor.author.fl_str_mv Cavadas, Miguel Alexandre dos Santos
dc.subject.por.fl_str_mv Gold nanoparticles
Antibody
Antigen
Bionanoconjugates
Diagnosis
topic Gold nanoparticles
Antibody
Antigen
Bionanoconjugates
Diagnosis
description Thesis for the Master degree in Structural and Functional Biochemistry
publishDate 2011
dc.date.none.fl_str_mv 2011
2011-01-01T00:00:00Z
2012-01-11T12:08:34Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/6673
url http://hdl.handle.net/10362/6673
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Faculdade de Ciências e Tecnologia
publisher.none.fl_str_mv Faculdade de Ciências e Tecnologia
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799137818134446080

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