Tyrosinase-based phenol remediation

Detalhes bibliográficos
Autor(a) principal: Gralheira, Daniel José Rossa
Data de Publicação: 2012
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/8951
Resumo: Dissertation for the obtention of the Master Degree in Biotechnology
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spelling Tyrosinase-based phenol remediationBionanoconjugatesGold nanoparticleRemediationTyrosinasePhenolDissertation for the obtention of the Master Degree in BiotechnologyNanobiotechnology is an emerging area full of potentialities to explore and reshape instituted knowledge. In this work, the main goal was to design and test biological remediation systems (a total of three) taking advantage of nanotechnology features, as a step towards future progress of sense-and-shoot combined systems. This task involved the development of active bionanoconjugates (BNC) with two different tyrosinases (Agaricus bisporus - AbT, and Tricoderma reesei - TrT) to be implemented in three suggested phenol remediation systems. AuNP-MUA (gold nanoparticles capped with11-mercapto-1-undecanoic acid) 1:5000 colloid was shown to be stable up to 100 mM ionic strength (sodium cloride) and able to form active BNCs with AbT. TrT:AuNP ratio for BNC formation is recommended as 100, supported by zeta potential and agarose gel mobility experimental data which, in turn, atone with the proposed theoretical estimation. The incubation of 100 ppm phenol with BNC-MUA-AbT for 60 minutes achieved 50% substrate conversion (the efficiency of the systems were also investigated using ultravioletvisible spectrophotometry (UV-Vis) complemented by standard addition method and solid-phase microextraction - gas chromatography (SPME-GC)).Faculdade de Ciências e TecnologiaCortez, JoãoSilva, MarcoRUNGralheira, Daniel José Rossa2013-02-22T14:50:18Z20122012-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10362/8951enginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T03:41:50Zoai:run.unl.pt:10362/8951Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:18:30.003600Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Tyrosinase-based phenol remediation
title Tyrosinase-based phenol remediation
spellingShingle Tyrosinase-based phenol remediation
Gralheira, Daniel José Rossa
Bionanoconjugates
Gold nanoparticle
Remediation
Tyrosinase
Phenol
title_short Tyrosinase-based phenol remediation
title_full Tyrosinase-based phenol remediation
title_fullStr Tyrosinase-based phenol remediation
title_full_unstemmed Tyrosinase-based phenol remediation
title_sort Tyrosinase-based phenol remediation
author Gralheira, Daniel José Rossa
author_facet Gralheira, Daniel José Rossa
author_role author
dc.contributor.none.fl_str_mv Cortez, João
Silva, Marco
RUN
dc.contributor.author.fl_str_mv Gralheira, Daniel José Rossa
dc.subject.por.fl_str_mv Bionanoconjugates
Gold nanoparticle
Remediation
Tyrosinase
Phenol
topic Bionanoconjugates
Gold nanoparticle
Remediation
Tyrosinase
Phenol
description Dissertation for the obtention of the Master Degree in Biotechnology
publishDate 2012
dc.date.none.fl_str_mv 2012
2012-01-01T00:00:00Z
2013-02-22T14:50:18Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/8951
url http://hdl.handle.net/10362/8951
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Faculdade de Ciências e Tecnologia
publisher.none.fl_str_mv Faculdade de Ciências e Tecnologia
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799137830893518848

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