Voltammetric immunosensor for the simultaneous analysis of the breast cancer biomarkers CA 15-3 and HER2-ECD

Detalhes bibliográficos
Autor(a) principal: Marques, Raquel C.B.
Data de Publicação: 2018
Outros Autores: Costa Rama, Estefanía, Viswanathan, Subramanian, Nouws, Henri, Costa-García, Agustín, Delerue-Matos, Cristina, González-García, M. Begoña
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.22/14706
Resumo: Cancer Antigen 15-3 (CA 15-3) and the extracellular domain of the human epidermal growth factor receptor 2 (HER2-ECD) are independent breast cancer biomarkers. The combination of their profiles (presence and concentration) could provide an important contribution to diagnostics and patient follow-up. Therefore, a disposable electrochemical immunosensor for the simultaneous detection of CA 15-3 and HER2-ECD was developed in this work. The immunosensor was constructed on a customized dual screen-printed carbon electrode. The carbon working electrodes' surfaces were first modified with in situ electrodeposited gold nanoparticles and then individually coated with either a monoclonal anti-human CA 15-3 or a monoclonal anti-human HER2-ECD antibody. After incubation with the biomarkers and monoclonal biotin-labelled detection antibodies, the antigen-antibody interactions were detected by linear sweep voltammetric analysis of enzymatically (alkaline phosphatase) generated metallic silver. The immunosensor’s limits of detection for the selected biomarkers were 5.0 U mL−1 for CA 15-3 and 2.9 ng mL−1 for HER2-ECD. These values could allow the use of the sensor in the non-invasive control of these biomarkers in breast cancer patients.
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spelling Voltammetric immunosensor for the simultaneous analysis of the breast cancer biomarkers CA 15-3 and HER2-ECDMultiplexed immunosensorElectrochemical immunosensorBreast cancer biomarkerCA 15-3HER2-ECDCancer Antigen 15-3 (CA 15-3) and the extracellular domain of the human epidermal growth factor receptor 2 (HER2-ECD) are independent breast cancer biomarkers. The combination of their profiles (presence and concentration) could provide an important contribution to diagnostics and patient follow-up. Therefore, a disposable electrochemical immunosensor for the simultaneous detection of CA 15-3 and HER2-ECD was developed in this work. The immunosensor was constructed on a customized dual screen-printed carbon electrode. The carbon working electrodes' surfaces were first modified with in situ electrodeposited gold nanoparticles and then individually coated with either a monoclonal anti-human CA 15-3 or a monoclonal anti-human HER2-ECD antibody. After incubation with the biomarkers and monoclonal biotin-labelled detection antibodies, the antigen-antibody interactions were detected by linear sweep voltammetric analysis of enzymatically (alkaline phosphatase) generated metallic silver. The immunosensor’s limits of detection for the selected biomarkers were 5.0 U mL−1 for CA 15-3 and 2.9 ng mL−1 for HER2-ECD. These values could allow the use of the sensor in the non-invasive control of these biomarkers in breast cancer patients.This work received financial support from the European Union (FEDER funds through COMPETE) and National Funds (FCT, Fundação para a Ciência e a Tecnologia) through projects PTDC/SAU-ENB/114786/2009 and UID/QUI/50006/2013. Estefanía Costa Rama thanks the Government of Principado de Asturias for the award of a Severo Ochoa predoctoral grant (BP11-097).ElsevierRepositório Científico do Instituto Politécnico do PortoMarques, Raquel C.B.Costa Rama, EstefaníaViswanathan, SubramanianNouws, HenriCosta-García, AgustínDelerue-Matos, CristinaGonzález-García, M. Begoña2019-10-07T13:41:28Z20182018-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.22/14706eng0925-400510.1016/j.snb.2017.08.107info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-13T12:57:48Zoai:recipp.ipp.pt:10400.22/14706Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:34:15.367211Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Voltammetric immunosensor for the simultaneous analysis of the breast cancer biomarkers CA 15-3 and HER2-ECD
title Voltammetric immunosensor for the simultaneous analysis of the breast cancer biomarkers CA 15-3 and HER2-ECD
spellingShingle Voltammetric immunosensor for the simultaneous analysis of the breast cancer biomarkers CA 15-3 and HER2-ECD
Marques, Raquel C.B.
Multiplexed immunosensor
Electrochemical immunosensor
Breast cancer biomarker
CA 15-3
HER2-ECD
title_short Voltammetric immunosensor for the simultaneous analysis of the breast cancer biomarkers CA 15-3 and HER2-ECD
title_full Voltammetric immunosensor for the simultaneous analysis of the breast cancer biomarkers CA 15-3 and HER2-ECD
title_fullStr Voltammetric immunosensor for the simultaneous analysis of the breast cancer biomarkers CA 15-3 and HER2-ECD
title_full_unstemmed Voltammetric immunosensor for the simultaneous analysis of the breast cancer biomarkers CA 15-3 and HER2-ECD
title_sort Voltammetric immunosensor for the simultaneous analysis of the breast cancer biomarkers CA 15-3 and HER2-ECD
author Marques, Raquel C.B.
author_facet Marques, Raquel C.B.
Costa Rama, Estefanía
Viswanathan, Subramanian
Nouws, Henri
Costa-García, Agustín
Delerue-Matos, Cristina
González-García, M. Begoña
author_role author
author2 Costa Rama, Estefanía
Viswanathan, Subramanian
Nouws, Henri
Costa-García, Agustín
Delerue-Matos, Cristina
González-García, M. Begoña
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório Científico do Instituto Politécnico do Porto
dc.contributor.author.fl_str_mv Marques, Raquel C.B.
Costa Rama, Estefanía
Viswanathan, Subramanian
Nouws, Henri
Costa-García, Agustín
Delerue-Matos, Cristina
González-García, M. Begoña
dc.subject.por.fl_str_mv Multiplexed immunosensor
Electrochemical immunosensor
Breast cancer biomarker
CA 15-3
HER2-ECD
topic Multiplexed immunosensor
Electrochemical immunosensor
Breast cancer biomarker
CA 15-3
HER2-ECD
description Cancer Antigen 15-3 (CA 15-3) and the extracellular domain of the human epidermal growth factor receptor 2 (HER2-ECD) are independent breast cancer biomarkers. The combination of their profiles (presence and concentration) could provide an important contribution to diagnostics and patient follow-up. Therefore, a disposable electrochemical immunosensor for the simultaneous detection of CA 15-3 and HER2-ECD was developed in this work. The immunosensor was constructed on a customized dual screen-printed carbon electrode. The carbon working electrodes' surfaces were first modified with in situ electrodeposited gold nanoparticles and then individually coated with either a monoclonal anti-human CA 15-3 or a monoclonal anti-human HER2-ECD antibody. After incubation with the biomarkers and monoclonal biotin-labelled detection antibodies, the antigen-antibody interactions were detected by linear sweep voltammetric analysis of enzymatically (alkaline phosphatase) generated metallic silver. The immunosensor’s limits of detection for the selected biomarkers were 5.0 U mL−1 for CA 15-3 and 2.9 ng mL−1 for HER2-ECD. These values could allow the use of the sensor in the non-invasive control of these biomarkers in breast cancer patients.
publishDate 2018
dc.date.none.fl_str_mv 2018
2018-01-01T00:00:00Z
2019-10-07T13:41:28Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.22/14706
url http://hdl.handle.net/10400.22/14706
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0925-4005
10.1016/j.snb.2017.08.107
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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