Label-free Voltammetric Immunosensor for Prostate Specific Antigen Detection

Detalhes bibliográficos
Autor(a) principal: Oliveira, Nélia
Data de Publicação: 2018
Outros Autores: Costa-Rama, Estefanía, Viswanathan, Subramanian, Delerue-Matos, Cristina, Pereira, Lourdes, Morais, Simone
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.22/14590
Resumo: This work describes a sensitive voltammetric immunosensor for label‐free electroanalysis of the prostate specific antigen (PSA), the main biomarker of prostate cancer. A gold electrode was firstly modified with the optimum self‐assembled monolayer (SAM), 1,6‐hexanedithiol, followed by the subsequent adsorption of gold nanoparticles (AuNPs) and, then, the monoclonal antibody to recognize PSA was immobilized. The influence of the most significant experimental variables (SAM type and incubation time, AuNPs deposition, antibody concentration and bovine serum albumin immobilization) on the biosensor response was studied by microscopy and voltammetry techniques. The electroanalytical detection was based on the interaction between PSA antibody and PSA via square‐wave voltammetry using ferrocyanide/ferricyanide as electrochemical redox indicator. Using the proposed immunosensor, PSA was specifically detected within the linear range between 0.2 and 200 ng mL−1 with 0.01 ng mL−1 as limit of detection. The immunosensor allows accurate, reproducible and sensitive (22.7 % reduction mL ng−1) detection in a concentration range useful for clinical purposes.
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spelling Label-free Voltammetric Immunosensor for Prostate Specific Antigen DetectionElectrochemical immunosensorSelf-assembled monolayerGold nanoparticlesCancer biomarkerProstate specific antigenThis work describes a sensitive voltammetric immunosensor for label‐free electroanalysis of the prostate specific antigen (PSA), the main biomarker of prostate cancer. A gold electrode was firstly modified with the optimum self‐assembled monolayer (SAM), 1,6‐hexanedithiol, followed by the subsequent adsorption of gold nanoparticles (AuNPs) and, then, the monoclonal antibody to recognize PSA was immobilized. The influence of the most significant experimental variables (SAM type and incubation time, AuNPs deposition, antibody concentration and bovine serum albumin immobilization) on the biosensor response was studied by microscopy and voltammetry techniques. The electroanalytical detection was based on the interaction between PSA antibody and PSA via square‐wave voltammetry using ferrocyanide/ferricyanide as electrochemical redox indicator. Using the proposed immunosensor, PSA was specifically detected within the linear range between 0.2 and 200 ng mL−1 with 0.01 ng mL−1 as limit of detection. The immunosensor allows accurate, reproducible and sensitive (22.7 % reduction mL ng−1) detection in a concentration range useful for clinical purposes.The authors are grateful for financial support from theEuropean Union (FEDER funds through COMPETE)and National Funds (FCT) through projects UID/QUI/50006/2013 and UID/CTM/50011/2013. The authors alsothank financial support by Norte Portugal RegionalOperational Programme (NORTE 2020), under thePORTUGAL 2020 Partnership Agreement, through theEuropean Regional Development Fund (ERDF): projectsNorte-01-0145-FEDER-000011, Norte-01-0145-FEDER-000024 and POCI-01-0145-FEDER-007679.Wiley-VCH VerlagRepositório Científico do Instituto Politécnico do PortoOliveira, NéliaCosta-Rama, EstefaníaViswanathan, SubramanianDelerue-Matos, CristinaPereira, LourdesMorais, Simone2019-09-13T09:10:09Z20182018-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.22/14590eng10.1002/elan.201800417info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-13T12:57:03Zoai:recipp.ipp.pt:10400.22/14590Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:34:05.794745Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Label-free Voltammetric Immunosensor for Prostate Specific Antigen Detection
title Label-free Voltammetric Immunosensor for Prostate Specific Antigen Detection
spellingShingle Label-free Voltammetric Immunosensor for Prostate Specific Antigen Detection
Oliveira, Nélia
Electrochemical immunosensor
Self-assembled monolayer
Gold nanoparticles
Cancer biomarker
Prostate specific antigen
title_short Label-free Voltammetric Immunosensor for Prostate Specific Antigen Detection
title_full Label-free Voltammetric Immunosensor for Prostate Specific Antigen Detection
title_fullStr Label-free Voltammetric Immunosensor for Prostate Specific Antigen Detection
title_full_unstemmed Label-free Voltammetric Immunosensor for Prostate Specific Antigen Detection
title_sort Label-free Voltammetric Immunosensor for Prostate Specific Antigen Detection
author Oliveira, Nélia
author_facet Oliveira, Nélia
Costa-Rama, Estefanía
Viswanathan, Subramanian
Delerue-Matos, Cristina
Pereira, Lourdes
Morais, Simone
author_role author
author2 Costa-Rama, Estefanía
Viswanathan, Subramanian
Delerue-Matos, Cristina
Pereira, Lourdes
Morais, Simone
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório Científico do Instituto Politécnico do Porto
dc.contributor.author.fl_str_mv Oliveira, Nélia
Costa-Rama, Estefanía
Viswanathan, Subramanian
Delerue-Matos, Cristina
Pereira, Lourdes
Morais, Simone
dc.subject.por.fl_str_mv Electrochemical immunosensor
Self-assembled monolayer
Gold nanoparticles
Cancer biomarker
Prostate specific antigen
topic Electrochemical immunosensor
Self-assembled monolayer
Gold nanoparticles
Cancer biomarker
Prostate specific antigen
description This work describes a sensitive voltammetric immunosensor for label‐free electroanalysis of the prostate specific antigen (PSA), the main biomarker of prostate cancer. A gold electrode was firstly modified with the optimum self‐assembled monolayer (SAM), 1,6‐hexanedithiol, followed by the subsequent adsorption of gold nanoparticles (AuNPs) and, then, the monoclonal antibody to recognize PSA was immobilized. The influence of the most significant experimental variables (SAM type and incubation time, AuNPs deposition, antibody concentration and bovine serum albumin immobilization) on the biosensor response was studied by microscopy and voltammetry techniques. The electroanalytical detection was based on the interaction between PSA antibody and PSA via square‐wave voltammetry using ferrocyanide/ferricyanide as electrochemical redox indicator. Using the proposed immunosensor, PSA was specifically detected within the linear range between 0.2 and 200 ng mL−1 with 0.01 ng mL−1 as limit of detection. The immunosensor allows accurate, reproducible and sensitive (22.7 % reduction mL ng−1) detection in a concentration range useful for clinical purposes.
publishDate 2018
dc.date.none.fl_str_mv 2018
2018-01-01T00:00:00Z
2019-09-13T09:10:09Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.22/14590
url http://hdl.handle.net/10400.22/14590
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1002/elan.201800417
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley-VCH Verlag
publisher.none.fl_str_mv Wiley-VCH Verlag
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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