Ca2+ monitoring in Plasmodium falciparum using the yellow cameleon-Nano biosensor
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/44851 |
Resumo: | Calcium (Ca2+)-mediated signaling is a conserved mechanism in eukaryotes, including the human malaria parasite, Plasmodium falciparum. Due to its small size (<10?µm) measurement of intracellular Ca2+ in Plasmodium is technically challenging, and thus Ca2+ regulation in this human pathogen is not well understood. Here we analyze Ca2+ homeostasis via a new approach using transgenic P. falciparum expressing the Ca2+ sensor yellow cameleon (YC)-Nano. We found that cytosolic Ca2+ concentration is maintained at low levels only during the intraerythrocytic trophozoite stage (30?nM), and is increased in the other blood stages (>300?nM). We determined that the mammalian SERCA inhibitor thapsigargin and antimalarial dihydroartemisinin did not perturb SERCA activity. The change of the cytosolic Ca2+ level in P. falciparum was additionally detectable by flow cytometry. Thus, we propose that the developed YC-Nano-based system is useful to study Ca2+ signaling in P. falciparum and is applicable for drug screening. |
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Ca2+ monitoring in Plasmodium falciparum using the yellow cameleon-Nano biosensorCiências Médicas::Medicina ClínicaScience & TechnologyCalcium (Ca2+)-mediated signaling is a conserved mechanism in eukaryotes, including the human malaria parasite, Plasmodium falciparum. Due to its small size (<10?µm) measurement of intracellular Ca2+ in Plasmodium is technically challenging, and thus Ca2+ regulation in this human pathogen is not well understood. Here we analyze Ca2+ homeostasis via a new approach using transgenic P. falciparum expressing the Ca2+ sensor yellow cameleon (YC)-Nano. We found that cytosolic Ca2+ concentration is maintained at low levels only during the intraerythrocytic trophozoite stage (30?nM), and is increased in the other blood stages (>300?nM). We determined that the mammalian SERCA inhibitor thapsigargin and antimalarial dihydroartemisinin did not perturb SERCA activity. The change of the cytosolic Ca2+ level in P. falciparum was additionally detectable by flow cytometry. Thus, we propose that the developed YC-Nano-based system is useful to study Ca2+ signaling in P. falciparum and is applicable for drug screening.We are grateful to Japanese Red Cross Blood Society for providing human RBC and plasma. We also thank Tanaka R, Ogoshi (Sakura) M and Matsumoto N for technical assistance and Templeton TJ for critical reading. This study was conducted at the Joint Usage / Research Center on Tropical Disease, Institute of Tropical Medicine, Nagasaki University, Japan. KP was a Tokyo Biochemical Research Foundation (TBRF, http://www.tokyobrf.or.jp) post-doctoral fellow and PEF was a Japanese Society of Promotion Sciences (JSPS) post-doctoral fellow. This work was supported in part by the TBRF (K.P.), JSPS (P.E.F.), Takeda Science Foundation (K.Y.), Grants-in-Aids for Scientific Research 24590509 (K.Y.), 22390079 (O.K.), and for Scientific Research on Innovative Areas 23117008 (O.K.), MEXT, Japan. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.Nature Publishing GroupUniversidade do MinhoPandey, KishorFerreira, Pedro Eduardo MendesIshikawa, TakeshiNagai, TakeharuKaneko, OsamuYahata, Kazuhide2016-032016-03-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/44851engPandey, K., Ferreira, P. E., Ishikawa, T., Nagai, T., Kaneko, O., & Yahata, K. (2016). Ca2+ monitoring in Plasmodium falciparum using the yellow cameleon-Nano biosensor. Scientific reports, 6. doi: 10.1038/srep234542045-232210.1038/srep2345427006284http://www.nature.com/articles/srep23454info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:44:59Zoai:repositorium.sdum.uminho.pt:1822/44851Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:42:46.942159Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Ca2+ monitoring in Plasmodium falciparum using the yellow cameleon-Nano biosensor |
title |
Ca2+ monitoring in Plasmodium falciparum using the yellow cameleon-Nano biosensor |
spellingShingle |
Ca2+ monitoring in Plasmodium falciparum using the yellow cameleon-Nano biosensor Pandey, Kishor Ciências Médicas::Medicina Clínica Science & Technology |
title_short |
Ca2+ monitoring in Plasmodium falciparum using the yellow cameleon-Nano biosensor |
title_full |
Ca2+ monitoring in Plasmodium falciparum using the yellow cameleon-Nano biosensor |
title_fullStr |
Ca2+ monitoring in Plasmodium falciparum using the yellow cameleon-Nano biosensor |
title_full_unstemmed |
Ca2+ monitoring in Plasmodium falciparum using the yellow cameleon-Nano biosensor |
title_sort |
Ca2+ monitoring in Plasmodium falciparum using the yellow cameleon-Nano biosensor |
author |
Pandey, Kishor |
author_facet |
Pandey, Kishor Ferreira, Pedro Eduardo Mendes Ishikawa, Takeshi Nagai, Takeharu Kaneko, Osamu Yahata, Kazuhide |
author_role |
author |
author2 |
Ferreira, Pedro Eduardo Mendes Ishikawa, Takeshi Nagai, Takeharu Kaneko, Osamu Yahata, Kazuhide |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Pandey, Kishor Ferreira, Pedro Eduardo Mendes Ishikawa, Takeshi Nagai, Takeharu Kaneko, Osamu Yahata, Kazuhide |
dc.subject.por.fl_str_mv |
Ciências Médicas::Medicina Clínica Science & Technology |
topic |
Ciências Médicas::Medicina Clínica Science & Technology |
description |
Calcium (Ca2+)-mediated signaling is a conserved mechanism in eukaryotes, including the human malaria parasite, Plasmodium falciparum. Due to its small size (<10?µm) measurement of intracellular Ca2+ in Plasmodium is technically challenging, and thus Ca2+ regulation in this human pathogen is not well understood. Here we analyze Ca2+ homeostasis via a new approach using transgenic P. falciparum expressing the Ca2+ sensor yellow cameleon (YC)-Nano. We found that cytosolic Ca2+ concentration is maintained at low levels only during the intraerythrocytic trophozoite stage (30?nM), and is increased in the other blood stages (>300?nM). We determined that the mammalian SERCA inhibitor thapsigargin and antimalarial dihydroartemisinin did not perturb SERCA activity. The change of the cytosolic Ca2+ level in P. falciparum was additionally detectable by flow cytometry. Thus, we propose that the developed YC-Nano-based system is useful to study Ca2+ signaling in P. falciparum and is applicable for drug screening. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-03 2016-03-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/44851 |
url |
http://hdl.handle.net/1822/44851 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Pandey, K., Ferreira, P. E., Ishikawa, T., Nagai, T., Kaneko, O., & Yahata, K. (2016). Ca2+ monitoring in Plasmodium falciparum using the yellow cameleon-Nano biosensor. Scientific reports, 6. doi: 10.1038/srep23454 2045-2322 10.1038/srep23454 27006284 http://www.nature.com/articles/srep23454 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Nature Publishing Group |
publisher.none.fl_str_mv |
Nature Publishing Group |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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