Sequential bioprocessing of Ulva rigida to produce lignocellulolytic enzymes and to improve its nutritional value as aquaculture feed

• Autor(a) principal: Fernandes, Helena
• Data de Publicação: 2019
• Outros Autores: Salgado, José Manuel, Martins, Nicole, Peres, Helena, Oliva-Teles, Aires, Belo, Isabel
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
• Texto Completo: http://hdl.handle.net/1822/59403
• topic: Ulva rigida; Solid-state fermentation; Enzymatic hydrolysis; Xylanase; Cellulase; Science & Technology

The macroalgae aquaculture industry has grown up in the last years, and new applications for macroalgae should be considered. In this work, sequential biological treatments as solid-state fermentation (SSF) by Aspergillus ibericus and enzymatic hydrolysis (EH) were applied to washed and unwashed Ulva rigida. SSF of unwashed macroalgae showed higher xylanase (359.8 U/g), cellulase (73.07 U/g) and β-glucosidase (14.9 U/g) activities per dry mass of macroalgae. After SSF, two strategies to carry out EH were assayed. The best process was SSF followed by EH by simply adding a buffer. The non-starch polysaccharides content was reduced by 93.2 %, achieving a glucan conversion of 98 %. In addition, the antioxidant activity was improved 2.8-fold and the protein concentration of macroalgae extracts increased from 16.9 % to 29.8 % (w/w). These biological treatments allowed to increase macroalgae value as feedstuff with potential for use in aquafeeds.