Selection of thermophilic and thermotolerant fungi for the production of cellulases and xylanases under solid-state fermentation
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Microbiology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300032 |
Resumo: | Twenty-seven thermophilic and thermotolerant fungal strains were isolated from soil, decaying organic matter and sugarcane piles based on their ability to grow at 45ºC on medium containing corn straw and cardboard as carbon sources. These fungi were identified in the genera Aspergillus, Thermomyces, Myceliophthora, Thermomucor and Candida. The majority of the isolated strains produced xylanase and cellulases under solid state fermentation (SSF). The highest cellulase and xylanase productions were obtained by the cultivation of the strains identified as Aspergillus fumigatus M.7.1 and Myceliophthora thermophila M.7.7. The enzymes from these strains exhibited maximum activity at pH 5.0 and at 60 and 70ºC. The endo-glucanase from A. fumigatus was stable from 40ºC to 65ºC and both endo-glucanase and xylanase from M. thermophila were stable in this temperature range when in absence of substrate. The enzymes were stable from pH 4.0 to 9.0. |
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Brazilian Journal of Microbiology |
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Selection of thermophilic and thermotolerant fungi for the production of cellulases and xylanases under solid-state fermentationThermophilic fungisolid-state fermentationxylanasecellulaseTwenty-seven thermophilic and thermotolerant fungal strains were isolated from soil, decaying organic matter and sugarcane piles based on their ability to grow at 45ºC on medium containing corn straw and cardboard as carbon sources. These fungi were identified in the genera Aspergillus, Thermomyces, Myceliophthora, Thermomucor and Candida. The majority of the isolated strains produced xylanase and cellulases under solid state fermentation (SSF). The highest cellulase and xylanase productions were obtained by the cultivation of the strains identified as Aspergillus fumigatus M.7.1 and Myceliophthora thermophila M.7.7. The enzymes from these strains exhibited maximum activity at pH 5.0 and at 60 and 70ºC. The endo-glucanase from A. fumigatus was stable from 40ºC to 65ºC and both endo-glucanase and xylanase from M. thermophila were stable in this temperature range when in absence of substrate. The enzymes were stable from pH 4.0 to 9.0.Sociedade Brasileira de Microbiologia2012-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300032Brazilian Journal of Microbiology v.43 n.3 2012reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822012000300032info:eu-repo/semantics/openAccessMoretti,Marcia M. S.Bocchini-Martins,Daniela A.Silva,Roberto DaRodrigues,AndréSette,Lara D.Gomes,Elenieng2012-11-26T00:00:00Zoai:scielo:S1517-83822012000300032Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2012-11-26T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false |
dc.title.none.fl_str_mv |
Selection of thermophilic and thermotolerant fungi for the production of cellulases and xylanases under solid-state fermentation |
title |
Selection of thermophilic and thermotolerant fungi for the production of cellulases and xylanases under solid-state fermentation |
spellingShingle |
Selection of thermophilic and thermotolerant fungi for the production of cellulases and xylanases under solid-state fermentation Moretti,Marcia M. S. Thermophilic fungi solid-state fermentation xylanase cellulase |
title_short |
Selection of thermophilic and thermotolerant fungi for the production of cellulases and xylanases under solid-state fermentation |
title_full |
Selection of thermophilic and thermotolerant fungi for the production of cellulases and xylanases under solid-state fermentation |
title_fullStr |
Selection of thermophilic and thermotolerant fungi for the production of cellulases and xylanases under solid-state fermentation |
title_full_unstemmed |
Selection of thermophilic and thermotolerant fungi for the production of cellulases and xylanases under solid-state fermentation |
title_sort |
Selection of thermophilic and thermotolerant fungi for the production of cellulases and xylanases under solid-state fermentation |
author |
Moretti,Marcia M. S. |
author_facet |
Moretti,Marcia M. S. Bocchini-Martins,Daniela A. Silva,Roberto Da Rodrigues,André Sette,Lara D. Gomes,Eleni |
author_role |
author |
author2 |
Bocchini-Martins,Daniela A. Silva,Roberto Da Rodrigues,André Sette,Lara D. Gomes,Eleni |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Moretti,Marcia M. S. Bocchini-Martins,Daniela A. Silva,Roberto Da Rodrigues,André Sette,Lara D. Gomes,Eleni |
dc.subject.por.fl_str_mv |
Thermophilic fungi solid-state fermentation xylanase cellulase |
topic |
Thermophilic fungi solid-state fermentation xylanase cellulase |
description |
Twenty-seven thermophilic and thermotolerant fungal strains were isolated from soil, decaying organic matter and sugarcane piles based on their ability to grow at 45ºC on medium containing corn straw and cardboard as carbon sources. These fungi were identified in the genera Aspergillus, Thermomyces, Myceliophthora, Thermomucor and Candida. The majority of the isolated strains produced xylanase and cellulases under solid state fermentation (SSF). The highest cellulase and xylanase productions were obtained by the cultivation of the strains identified as Aspergillus fumigatus M.7.1 and Myceliophthora thermophila M.7.7. The enzymes from these strains exhibited maximum activity at pH 5.0 and at 60 and 70ºC. The endo-glucanase from A. fumigatus was stable from 40ºC to 65ºC and both endo-glucanase and xylanase from M. thermophila were stable in this temperature range when in absence of substrate. The enzymes were stable from pH 4.0 to 9.0. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-09-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300032 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300032 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1517-83822012000300032 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
dc.source.none.fl_str_mv |
Brazilian Journal of Microbiology v.43 n.3 2012 reponame:Brazilian Journal of Microbiology instname:Sociedade Brasileira de Microbiologia (SBM) instacron:SBM |
instname_str |
Sociedade Brasileira de Microbiologia (SBM) |
instacron_str |
SBM |
institution |
SBM |
reponame_str |
Brazilian Journal of Microbiology |
collection |
Brazilian Journal of Microbiology |
repository.name.fl_str_mv |
Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM) |
repository.mail.fl_str_mv |
bjm@sbmicrobiologia.org.br||mbmartin@usp.br |
_version_ |
1752122204724658176 |