Haemophilus influenzae Carriage among Healthy Children in Portugal, 2015-2019

Detalhes bibliográficos
Autor(a) principal: Bajanca-Lavado, Maria Paula
Data de Publicação: 2022
Outros Autores: Cavaco, Luís, Fernandes, Mariana, Touret, Tiago, Candeias, Catarina, Simões, Alexandra S., Sá-Leão, Raquel
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.18/8375
Resumo: This article belongs to the Special Issue Haemophilus influenzae: New Insights in Epidemiology of Disease.
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spelling Haemophilus influenzae Carriage among Healthy Children in Portugal, 2015-2019Haemophilus influenzaeSerotypeMLSTCarriageSurveillanceAntimicrobial ResistanceCapsuleWhole Genome SequencingDay-care CenterPortugalInfecções RespiratóriasThis article belongs to the Special Issue Haemophilus influenzae: New Insights in Epidemiology of Disease.Haemophilus influenzae is an important cause of mucosal and invasive infections and a common colonizer of the upper respiratory tract. As there are no recent data on H. influenzae carriage in Portugal, we aimed to characterize carriage samples and investigate possible parallelisms with disease isolates. Between 2016–2019, 1524 nasopharyngeal samples were obtained from children (0–6 years) attending day-care. H. influenzae were serotyped and screened for β-lactamase production. Strains producing β-lactamase and/or those that were encapsulated were further characterized by antibiotype; encapsulated strains were also investigated for MLST and the presence of antimicrobial resistance and virulence genes (extracted from whole genome sequencing). The overall carriage rate was 84.1%. Most isolates (96.7%) were nonencapsulated. Encapsulated strains were of serotypes f (1.8%), e (1.1%), a (0.3%), and b (0.1%). MLST showed clonality within serotypes. Although the lineages were the same as those that were described among disease isolates, colonization isolates had fewer virulence determinants. Overall, 7.5% of the isolates were β-lactamase positive; one isolate had blaTEM-82, which has not been previously described in H. influenzae. A single isolate, which was identified as H. parainfluenzae, had an incomplete f-like cap locus. In conclusion, circulation of serotype b is residual. The few encapsulated strains are genetically related to disease-causing isolates. Thus, surveillance of H. influenzae carriage should be maintained.This work was partially supported by projects LISBOA-01-0145-FEDER (Microbiologia Molecular, Estrutural e Celular, funded by FEDER through COMPETE2020—Programa Operacional Competitividade e Internacionalização), LISBOA-01-0145-FEDER-016417 (ONEIDA co-funded by Fundos Europeus Estruturais e de Investimento, Programa Operacional Regional Lisboa 2020 and Fundação para a Ciência e a Tecnologia (FCT)), WI230921 from Pfizer Portugal (to RSL), and National Institute of Health, Lisbon, Portugal. CC was supported by grant from Fundação para a Ciência e a Tecnologia, Portugal.MDPIRepositório Científico do Instituto Nacional de SaúdeBajanca-Lavado, Maria PaulaCavaco, LuísFernandes, MarianaTouret, TiagoCandeias, CatarinaSimões, Alexandra S.Sá-Leão, Raquel2022-12-02T15:47:32Z2022-10-042022-10-04T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.18/8375engMicroorganisms. 2022 Oct 4;10(10):1964. doi: 10.3390/microorganisms101019642076-260710.3390/microorganisms10101964info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-20T15:42:31Zoai:repositorio.insa.pt:10400.18/8375Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:42:59.868908Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Haemophilus influenzae Carriage among Healthy Children in Portugal, 2015-2019
title Haemophilus influenzae Carriage among Healthy Children in Portugal, 2015-2019
spellingShingle Haemophilus influenzae Carriage among Healthy Children in Portugal, 2015-2019
Bajanca-Lavado, Maria Paula
Haemophilus influenzae
Serotype
MLST
Carriage
Surveillance
Antimicrobial Resistance
Capsule
Whole Genome Sequencing
Day-care Center
Portugal
Infecções Respiratórias
title_short Haemophilus influenzae Carriage among Healthy Children in Portugal, 2015-2019
title_full Haemophilus influenzae Carriage among Healthy Children in Portugal, 2015-2019
title_fullStr Haemophilus influenzae Carriage among Healthy Children in Portugal, 2015-2019
title_full_unstemmed Haemophilus influenzae Carriage among Healthy Children in Portugal, 2015-2019
title_sort Haemophilus influenzae Carriage among Healthy Children in Portugal, 2015-2019
author Bajanca-Lavado, Maria Paula
author_facet Bajanca-Lavado, Maria Paula
Cavaco, Luís
Fernandes, Mariana
Touret, Tiago
Candeias, Catarina
Simões, Alexandra S.
Sá-Leão, Raquel
author_role author
author2 Cavaco, Luís
Fernandes, Mariana
Touret, Tiago
Candeias, Catarina
Simões, Alexandra S.
Sá-Leão, Raquel
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório Científico do Instituto Nacional de Saúde
dc.contributor.author.fl_str_mv Bajanca-Lavado, Maria Paula
Cavaco, Luís
Fernandes, Mariana
Touret, Tiago
Candeias, Catarina
Simões, Alexandra S.
Sá-Leão, Raquel
dc.subject.por.fl_str_mv Haemophilus influenzae
Serotype
MLST
Carriage
Surveillance
Antimicrobial Resistance
Capsule
Whole Genome Sequencing
Day-care Center
Portugal
Infecções Respiratórias
topic Haemophilus influenzae
Serotype
MLST
Carriage
Surveillance
Antimicrobial Resistance
Capsule
Whole Genome Sequencing
Day-care Center
Portugal
Infecções Respiratórias
description This article belongs to the Special Issue Haemophilus influenzae: New Insights in Epidemiology of Disease.
publishDate 2022
dc.date.none.fl_str_mv 2022-12-02T15:47:32Z
2022-10-04
2022-10-04T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.18/8375
url http://hdl.handle.net/10400.18/8375
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Microorganisms. 2022 Oct 4;10(10):1964. doi: 10.3390/microorganisms10101964
2076-2607
10.3390/microorganisms10101964
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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