Microbiota fingerprints within the oral cavity of cetaceans as indicators for population biomonitoring

Detalhes bibliográficos
Autor(a) principal: Soares-Castro, Pedro
Data de Publicação: 2019
Outros Autores: Araújo-Rodrigues, Helena, Godoy-Vitorino, Filipa, Ferreira, Marisa, Covelo, Pablo, López, Alfredo, Vingada, José, Eira, Catarina, Santos, P. M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/73224
Resumo: The composition of mammalian microbiota has been related with the host health status. In this study, we assessed the oral microbiome of 3 cetacean species most commonly found stranded in Iberian Atlantic waters (Delphinus delphis, Stenella coeruleoalba and Phocoena phocoena), using 16S rDNA-amplicon metabarcoding. All oral microbiomes were dominated by Proteobacteria, Firmicutes, Bacteroidetes and Fusobacteria bacteria, which were also predominant in the oral cavity of Tursiops truncatus. A Constrained Canonical Analysis (CCA) showed that the major factors shaping the composition of 38 oral microbiomes (p-value < 0.05) were: (i) animal species and (ii) age class, segregating adults and juveniles. The correlation analysis also grouped the microbiomes by animal stranding location and health status. Similar discriminatory patterns were detected using the data from a previous study on Tursiops truncatus, indicating that this correlation approach may facilitate data comparisons between different studies on several cetacean species. This study identified a total of 15 bacterial genera and 27 OTUs discriminating between the observed CCA groups, which can be further explored as microbiota fingerprints to develop (i) specific diagnostic assays for cetacean population conservation and (ii) bio-monitoring approaches to assess the health of marine ecosystems from the Iberian Atlantic basin, using cetaceans as bioindicators.
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spelling Microbiota fingerprints within the oral cavity of cetaceans as indicators for population biomonitoringAnimalsBiological MonitoringCetaceaDNA Barcoding, TaxonomicMicrobiotaMouthCiências Naturais::Ciências BiológicasScience & TechnologyThe composition of mammalian microbiota has been related with the host health status. In this study, we assessed the oral microbiome of 3 cetacean species most commonly found stranded in Iberian Atlantic waters (Delphinus delphis, Stenella coeruleoalba and Phocoena phocoena), using 16S rDNA-amplicon metabarcoding. All oral microbiomes were dominated by Proteobacteria, Firmicutes, Bacteroidetes and Fusobacteria bacteria, which were also predominant in the oral cavity of Tursiops truncatus. A Constrained Canonical Analysis (CCA) showed that the major factors shaping the composition of 38 oral microbiomes (p-value < 0.05) were: (i) animal species and (ii) age class, segregating adults and juveniles. The correlation analysis also grouped the microbiomes by animal stranding location and health status. Similar discriminatory patterns were detected using the data from a previous study on Tursiops truncatus, indicating that this correlation approach may facilitate data comparisons between different studies on several cetacean species. This study identified a total of 15 bacterial genera and 27 OTUs discriminating between the observed CCA groups, which can be further explored as microbiota fingerprints to develop (i) specific diagnostic assays for cetacean population conservation and (ii) bio-monitoring approaches to assess the health of marine ecosystems from the Iberian Atlantic basin, using cetaceans as bioindicators.The authors would like to acknowledge to Jorg Becker and Joao Sobral for the sequencing services provided at the Instituto Gulbenkian de Ciencia. This work was supported by the project CetSenti RECI/AAGGLO/0470/2012 (FCOMP-01-0124-FEDER-027472), by the strategic program UID/BIA/04050/2013 (POCI-01-0145-FEDER-007569), by the GenomePT project (POCI-01-0145-FEDER-022184), supported by COMPETE 2020 -Operational Programme for Competitiveness and Internationalisation (POCI), Lisboa Portugal Regional Operational Programme (Lisboa2020), Algarve Portugal Regional Operational Programme (CRESC Algarve2020), under the PORTUGAL 2020 Partnership Agreement, funded by national funds through the Fundacao para a Ciencia e a Tecnologia (FCT) I.P. and the European Regional Development Fund (ERDF), through the COMPETE2020-Programa Operacional Competitividade e Internacionalizacao (POCI). The work was supported as well through CESAM UID/AMB/50017/2013 co-funded by the FCT/MEC and FEDER within PT2020 and Compete 2020 (C.E.), FCT grants SFRH/BD/30240/2006 (M.F.), and SFRH/BPD/82407/2011 (A.L.) and NIH RCMI Grant No. 2U54MD007600 (F.G.V.).Nature ResearchUniversidade do MinhoSoares-Castro, PedroAraújo-Rodrigues, HelenaGodoy-Vitorino, FilipaFerreira, MarisaCovelo, PabloLópez, AlfredoVingada, JoséEira, CatarinaSantos, P. M.20192019-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/73224engSoares-Castro, P., Araújo-Rodrigues, H., Godoy-Vitorino, F. et al. Microbiota fingerprints within the oral cavity of cetaceans as indicators for population biomonitoring. Sci Rep 9, 13679 (2019). https://doi.org/10.1038/s41598-019-50139-72045-232210.1038/s41598-019-50139-731548611https://www.nature.com/articles/s41598-019-50139-7info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:09:42Zoai:repositorium.sdum.uminho.pt:1822/73224Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:01:11.095566Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Microbiota fingerprints within the oral cavity of cetaceans as indicators for population biomonitoring
title Microbiota fingerprints within the oral cavity of cetaceans as indicators for population biomonitoring
spellingShingle Microbiota fingerprints within the oral cavity of cetaceans as indicators for population biomonitoring
Soares-Castro, Pedro
Animals
Biological Monitoring
Cetacea
DNA Barcoding, Taxonomic
Microbiota
Mouth
Ciências Naturais::Ciências Biológicas
Science & Technology
title_short Microbiota fingerprints within the oral cavity of cetaceans as indicators for population biomonitoring
title_full Microbiota fingerprints within the oral cavity of cetaceans as indicators for population biomonitoring
title_fullStr Microbiota fingerprints within the oral cavity of cetaceans as indicators for population biomonitoring
title_full_unstemmed Microbiota fingerprints within the oral cavity of cetaceans as indicators for population biomonitoring
title_sort Microbiota fingerprints within the oral cavity of cetaceans as indicators for population biomonitoring
author Soares-Castro, Pedro
author_facet Soares-Castro, Pedro
Araújo-Rodrigues, Helena
Godoy-Vitorino, Filipa
Ferreira, Marisa
Covelo, Pablo
López, Alfredo
Vingada, José
Eira, Catarina
Santos, P. M.
author_role author
author2 Araújo-Rodrigues, Helena
Godoy-Vitorino, Filipa
Ferreira, Marisa
Covelo, Pablo
López, Alfredo
Vingada, José
Eira, Catarina
Santos, P. M.
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Soares-Castro, Pedro
Araújo-Rodrigues, Helena
Godoy-Vitorino, Filipa
Ferreira, Marisa
Covelo, Pablo
López, Alfredo
Vingada, José
Eira, Catarina
Santos, P. M.
dc.subject.por.fl_str_mv Animals
Biological Monitoring
Cetacea
DNA Barcoding, Taxonomic
Microbiota
Mouth
Ciências Naturais::Ciências Biológicas
Science & Technology
topic Animals
Biological Monitoring
Cetacea
DNA Barcoding, Taxonomic
Microbiota
Mouth
Ciências Naturais::Ciências Biológicas
Science & Technology
description The composition of mammalian microbiota has been related with the host health status. In this study, we assessed the oral microbiome of 3 cetacean species most commonly found stranded in Iberian Atlantic waters (Delphinus delphis, Stenella coeruleoalba and Phocoena phocoena), using 16S rDNA-amplicon metabarcoding. All oral microbiomes were dominated by Proteobacteria, Firmicutes, Bacteroidetes and Fusobacteria bacteria, which were also predominant in the oral cavity of Tursiops truncatus. A Constrained Canonical Analysis (CCA) showed that the major factors shaping the composition of 38 oral microbiomes (p-value < 0.05) were: (i) animal species and (ii) age class, segregating adults and juveniles. The correlation analysis also grouped the microbiomes by animal stranding location and health status. Similar discriminatory patterns were detected using the data from a previous study on Tursiops truncatus, indicating that this correlation approach may facilitate data comparisons between different studies on several cetacean species. This study identified a total of 15 bacterial genera and 27 OTUs discriminating between the observed CCA groups, which can be further explored as microbiota fingerprints to develop (i) specific diagnostic assays for cetacean population conservation and (ii) bio-monitoring approaches to assess the health of marine ecosystems from the Iberian Atlantic basin, using cetaceans as bioindicators.
publishDate 2019
dc.date.none.fl_str_mv 2019
2019-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/73224
url http://hdl.handle.net/1822/73224
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Soares-Castro, P., Araújo-Rodrigues, H., Godoy-Vitorino, F. et al. Microbiota fingerprints within the oral cavity of cetaceans as indicators for population biomonitoring. Sci Rep 9, 13679 (2019). https://doi.org/10.1038/s41598-019-50139-7
2045-2322
10.1038/s41598-019-50139-7
31548611
https://www.nature.com/articles/s41598-019-50139-7
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Nature Research
publisher.none.fl_str_mv Nature Research
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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