A new approach to bacterial colony morphotyping by matrix-assisted laser desorption ionization time of flight-based mass spectrometry

Detalhes bibliográficos
Autor(a) principal: Sousa, Ana Margarida
Data de Publicação: 2013
Outros Autores: Nunes-Miranda, J. D., Reboiro-Jato, M., Fdez-Riverola, Florentino, Lourenço, Anália, Pereira, Maria Olívia, Capelo, José Luis
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/25064
Resumo: Matrix assisted laser desorption ionization time of flight mass spectrometry has been explored as a tool to bacterial colony morphotyping.To this end,four colony morphotypes of Pseudomonas aeruginosa and four of Staphylococcus aureus were analysed using intact bacteria.Results suggest that mass spectrometry of intact bacteria could,in some extent,be used to complement the classical morphological classification of bacteria.
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spelling A new approach to bacterial colony morphotyping by matrix-assisted laser desorption ionization time of flight-based mass spectrometryBacteriaMALDIMorphotypeClassificationKeywords Bacteria MALDI Morphotype ClassificationScience & TechnologyMatrix assisted laser desorption ionization time of flight mass spectrometry has been explored as a tool to bacterial colony morphotyping.To this end,four colony morphotypes of Pseudomonas aeruginosa and four of Staphylococcus aureus were analysed using intact bacteria.Results suggest that mass spectrometry of intact bacteria could,in some extent,be used to complement the classical morphological classification of bacteria.Financial support from IBB-CEB and Fundacao para a Ciencia e Tecnologia (FCT) and European Community fund FEDER, through Program COMPETE, in the ambit of the FCT project "PTDC/SAU-SAP/113196/2009/ FCOMP-01-0124-FEDER-016012" and Ana Margarida Sousa PhD grant (SFRH/BD/72551/2010), is gratefully acknowledged. Authors also thank Portugal-Spain cooperation action sponsored by the Foundation of Portuguese Universities [E 48/11] and the Spanish Ministry of Science and Innovation [AIB2010PT-00353].ElsevierElsevier BVUniversidade do MinhoSousa, Ana MargaridaNunes-Miranda, J. D.Reboiro-Jato, M.Fdez-Riverola, FlorentinoLourenço, AnáliaPereira, Maria OlíviaCapelo, José Luis20132013-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/25064eng0039-914010.1016/j.talanta.2013.04.05824148379info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:21:03Zoai:repositorium.sdum.uminho.pt:1822/25064Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:14:14.060411Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv A new approach to bacterial colony morphotyping by matrix-assisted laser desorption ionization time of flight-based mass spectrometry
title A new approach to bacterial colony morphotyping by matrix-assisted laser desorption ionization time of flight-based mass spectrometry
spellingShingle A new approach to bacterial colony morphotyping by matrix-assisted laser desorption ionization time of flight-based mass spectrometry
Sousa, Ana Margarida
Bacteria
MALDI
Morphotype
Classification
Keywords Bacteria MALDI Morphotype Classification
Science & Technology
title_short A new approach to bacterial colony morphotyping by matrix-assisted laser desorption ionization time of flight-based mass spectrometry
title_full A new approach to bacterial colony morphotyping by matrix-assisted laser desorption ionization time of flight-based mass spectrometry
title_fullStr A new approach to bacterial colony morphotyping by matrix-assisted laser desorption ionization time of flight-based mass spectrometry
title_full_unstemmed A new approach to bacterial colony morphotyping by matrix-assisted laser desorption ionization time of flight-based mass spectrometry
title_sort A new approach to bacterial colony morphotyping by matrix-assisted laser desorption ionization time of flight-based mass spectrometry
author Sousa, Ana Margarida
author_facet Sousa, Ana Margarida
Nunes-Miranda, J. D.
Reboiro-Jato, M.
Fdez-Riverola, Florentino
Lourenço, Anália
Pereira, Maria Olívia
Capelo, José Luis
author_role author
author2 Nunes-Miranda, J. D.
Reboiro-Jato, M.
Fdez-Riverola, Florentino
Lourenço, Anália
Pereira, Maria Olívia
Capelo, José Luis
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Sousa, Ana Margarida
Nunes-Miranda, J. D.
Reboiro-Jato, M.
Fdez-Riverola, Florentino
Lourenço, Anália
Pereira, Maria Olívia
Capelo, José Luis
dc.subject.por.fl_str_mv Bacteria
MALDI
Morphotype
Classification
Keywords Bacteria MALDI Morphotype Classification
Science & Technology
topic Bacteria
MALDI
Morphotype
Classification
Keywords Bacteria MALDI Morphotype Classification
Science & Technology
description Matrix assisted laser desorption ionization time of flight mass spectrometry has been explored as a tool to bacterial colony morphotyping.To this end,four colony morphotypes of Pseudomonas aeruginosa and four of Staphylococcus aureus were analysed using intact bacteria.Results suggest that mass spectrometry of intact bacteria could,in some extent,be used to complement the classical morphological classification of bacteria.
publishDate 2013
dc.date.none.fl_str_mv 2013
2013-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/25064
url http://hdl.handle.net/1822/25064
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0039-9140
10.1016/j.talanta.2013.04.058
24148379
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
Elsevier BV
publisher.none.fl_str_mv Elsevier
Elsevier BV
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
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instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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