A high throughput colorimetric assay of beta-1,3-D-glucans by Congo red dye

Detalhes bibliográficos
Autor(a) principal: Semedo, Madga Sofia Cardoso Nobre
Data de Publicação: 2015
Outros Autores: Karmali, Amin, Fonseca, Luís
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.21/5812
Resumo: Mushroom strains contain complex nutritional biomolecules with a wide spectrum of therapeutic and prophylactic properties. Among these compounds, β-d-glucans play an important role in immuno-modulating and anti-tumor activities. The present work involves a novel colorimetric assay method for β-1,3-d-glucans with a triple helix tertiary structure by using Congo red. The specific interaction that occurs between Congo red and β-1,3-d-glucan was detected by bathochromic shift from 488 to 516 nm (> 20 nm) in UV–Vis spectrophotometer. A micro- and high throughput method based on a 96-well microtiter plate was devised which presents several advantages over the published methods since it requires only 1.51 μg of polysaccharides in samples, greater sensitivity, speed, assay of many samples and very cheap. β-d-Glucans of several mushrooms (i.e., Coriolus versicolor, Ganoderma lucidum, Pleurotus ostreatus, Ganoderma carnosum, Hericium erinaceus, Lentinula edodes, Inonotus obliquus, Auricularia auricular, Polyporus umbellatus, Cordyseps sinensis, Agaricus blazei, Poria cocos) were isolated by using a sequence of several extractions with cold and boiling water, acidic and alkaline conditions and quantified by this microtiter plate method. FTIR spectroscopy was used to study the structural features of β-1,3-d-glucans in these mushroom samples as well as the specific interaction of these polysaccharides with Congo red. The effect of NaOH on triple helix conformation of β-1,3-d-glucans was investigated in several mushroom species.
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spelling A high throughput colorimetric assay of beta-1,3-D-glucans by Congo red dyeCongo red dyeMushroom strainsbeta-1,3-D-GlucanHigh-throughput assay96-Well microtiter plateTriple helix structureMushroom strains contain complex nutritional biomolecules with a wide spectrum of therapeutic and prophylactic properties. Among these compounds, β-d-glucans play an important role in immuno-modulating and anti-tumor activities. The present work involves a novel colorimetric assay method for β-1,3-d-glucans with a triple helix tertiary structure by using Congo red. The specific interaction that occurs between Congo red and β-1,3-d-glucan was detected by bathochromic shift from 488 to 516 nm (> 20 nm) in UV–Vis spectrophotometer. A micro- and high throughput method based on a 96-well microtiter plate was devised which presents several advantages over the published methods since it requires only 1.51 μg of polysaccharides in samples, greater sensitivity, speed, assay of many samples and very cheap. β-d-Glucans of several mushrooms (i.e., Coriolus versicolor, Ganoderma lucidum, Pleurotus ostreatus, Ganoderma carnosum, Hericium erinaceus, Lentinula edodes, Inonotus obliquus, Auricularia auricular, Polyporus umbellatus, Cordyseps sinensis, Agaricus blazei, Poria cocos) were isolated by using a sequence of several extractions with cold and boiling water, acidic and alkaline conditions and quantified by this microtiter plate method. FTIR spectroscopy was used to study the structural features of β-1,3-d-glucans in these mushroom samples as well as the specific interaction of these polysaccharides with Congo red. The effect of NaOH on triple helix conformation of β-1,3-d-glucans was investigated in several mushroom species.Elsevier B.V.RCIPLSemedo, Madga Sofia Cardoso NobreKarmali, AminFonseca, Luís2016-03-09T12:42:42Z2015-022015-02-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.21/5812engSEMEDO, M. C.; KARMALI, A.; FONSECA, L. - A high throughput colorimetric assay of beta-1,3-D-glucans by Congo red dye. Journal of Microbiological Methods. ISSN. 0167-7012. Vol. 109 (2015), pp. 140-1480167-701210.1016/j.mimet.2014.12.020metadata only accessinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-08-03T09:49:45Zoai:repositorio.ipl.pt:10400.21/5812Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:15:02.532151Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv A high throughput colorimetric assay of beta-1,3-D-glucans by Congo red dye
title A high throughput colorimetric assay of beta-1,3-D-glucans by Congo red dye
spellingShingle A high throughput colorimetric assay of beta-1,3-D-glucans by Congo red dye
Semedo, Madga Sofia Cardoso Nobre
Congo red dye
Mushroom strains
beta-1,3-D-Glucan
High-throughput assay
96-Well microtiter plate
Triple helix structure
title_short A high throughput colorimetric assay of beta-1,3-D-glucans by Congo red dye
title_full A high throughput colorimetric assay of beta-1,3-D-glucans by Congo red dye
title_fullStr A high throughput colorimetric assay of beta-1,3-D-glucans by Congo red dye
title_full_unstemmed A high throughput colorimetric assay of beta-1,3-D-glucans by Congo red dye
title_sort A high throughput colorimetric assay of beta-1,3-D-glucans by Congo red dye
author Semedo, Madga Sofia Cardoso Nobre
author_facet Semedo, Madga Sofia Cardoso Nobre
Karmali, Amin
Fonseca, Luís
author_role author
author2 Karmali, Amin
Fonseca, Luís
author2_role author
author
dc.contributor.none.fl_str_mv RCIPL
dc.contributor.author.fl_str_mv Semedo, Madga Sofia Cardoso Nobre
Karmali, Amin
Fonseca, Luís
dc.subject.por.fl_str_mv Congo red dye
Mushroom strains
beta-1,3-D-Glucan
High-throughput assay
96-Well microtiter plate
Triple helix structure
topic Congo red dye
Mushroom strains
beta-1,3-D-Glucan
High-throughput assay
96-Well microtiter plate
Triple helix structure
description Mushroom strains contain complex nutritional biomolecules with a wide spectrum of therapeutic and prophylactic properties. Among these compounds, β-d-glucans play an important role in immuno-modulating and anti-tumor activities. The present work involves a novel colorimetric assay method for β-1,3-d-glucans with a triple helix tertiary structure by using Congo red. The specific interaction that occurs between Congo red and β-1,3-d-glucan was detected by bathochromic shift from 488 to 516 nm (> 20 nm) in UV–Vis spectrophotometer. A micro- and high throughput method based on a 96-well microtiter plate was devised which presents several advantages over the published methods since it requires only 1.51 μg of polysaccharides in samples, greater sensitivity, speed, assay of many samples and very cheap. β-d-Glucans of several mushrooms (i.e., Coriolus versicolor, Ganoderma lucidum, Pleurotus ostreatus, Ganoderma carnosum, Hericium erinaceus, Lentinula edodes, Inonotus obliquus, Auricularia auricular, Polyporus umbellatus, Cordyseps sinensis, Agaricus blazei, Poria cocos) were isolated by using a sequence of several extractions with cold and boiling water, acidic and alkaline conditions and quantified by this microtiter plate method. FTIR spectroscopy was used to study the structural features of β-1,3-d-glucans in these mushroom samples as well as the specific interaction of these polysaccharides with Congo red. The effect of NaOH on triple helix conformation of β-1,3-d-glucans was investigated in several mushroom species.
publishDate 2015
dc.date.none.fl_str_mv 2015-02
2015-02-01T00:00:00Z
2016-03-09T12:42:42Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.21/5812
url http://hdl.handle.net/10400.21/5812
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv SEMEDO, M. C.; KARMALI, A.; FONSECA, L. - A high throughput colorimetric assay of beta-1,3-D-glucans by Congo red dye. Journal of Microbiological Methods. ISSN. 0167-7012. Vol. 109 (2015), pp. 140-148
0167-7012
10.1016/j.mimet.2014.12.020
dc.rights.driver.fl_str_mv metadata only access
info:eu-repo/semantics/openAccess
rights_invalid_str_mv metadata only access
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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