In vitro antifungal effect of EDTA disodium salt in tested black aspergilli

Detalhes bibliográficos
Autor(a) principal: Abrunhosa, Luís
Data de Publicação: 2008
Outros Autores: Venâncio, Armando
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/7974
Resumo: The antifungal effect of Na2EDTA on an Aspergillus carbonarius, an A. ibericus, an ochratoxigenic A. niger and a non-ochratoxigenic A. niger strain was studied. Also, the effect of Na2EDTA on the production of ochratoxin A by A. carbonarius and the ochratoxigenic A. niger was evaluated. The poisoned food technique was used with CYA medium supplemented with 0, 1 and 10 mmol L-1 of Na2EDTA. The colony diameters were recorded daily and the amount of ochratoxin A produced was quantified every two days. Significant reductions of growth rates were observed in the presence of Na2EDTA being the calculated EC50 of 2.1 mmol L-1 for A. carbonarius, 0.9 mmol L-1 for A. ibericus, 2.0 mmol L-1 for the ochratoxigenic A. niger and 4.1 mmol L-1 for the non-ochratoxigenic A. niger. Furthermore, 10 mmol L-1 Na2EDTA delayed the production of ochratoxin A and reduced the levels in approximately 99% during 8 days. Na2EDTA is frequently used in the food industry and in agriculture agrochemicals and its effects on ochratoxigenic black aspergilli is not well known. This study showed that Na2EDTA can significantly reduce the growth rates of tested fungi and its ochratoxin A production.
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spelling In vitro antifungal effect of EDTA disodium salt in tested black aspergilliAspergrlIus carbonariusAspergiIlus ibericusAspergillus nigerOchratoxin AEDTAFungicideAspergillus carbonariusAspergillus ibericusThe antifungal effect of Na2EDTA on an Aspergillus carbonarius, an A. ibericus, an ochratoxigenic A. niger and a non-ochratoxigenic A. niger strain was studied. Also, the effect of Na2EDTA on the production of ochratoxin A by A. carbonarius and the ochratoxigenic A. niger was evaluated. The poisoned food technique was used with CYA medium supplemented with 0, 1 and 10 mmol L-1 of Na2EDTA. The colony diameters were recorded daily and the amount of ochratoxin A produced was quantified every two days. Significant reductions of growth rates were observed in the presence of Na2EDTA being the calculated EC50 of 2.1 mmol L-1 for A. carbonarius, 0.9 mmol L-1 for A. ibericus, 2.0 mmol L-1 for the ochratoxigenic A. niger and 4.1 mmol L-1 for the non-ochratoxigenic A. niger. Furthermore, 10 mmol L-1 Na2EDTA delayed the production of ochratoxin A and reduced the levels in approximately 99% during 8 days. Na2EDTA is frequently used in the food industry and in agriculture agrochemicals and its effects on ochratoxigenic black aspergilli is not well known. This study showed that Na2EDTA can significantly reduce the growth rates of tested fungi and its ochratoxin A production.Fundação para a Ciência e a Tecnologia (FCT)Academic Journals Inc.Universidade do MinhoAbrunhosa, LuísVenâncio, Armando20082008-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/7974eng"Asian Journal of Biochemistry". ISSN 1815-9923. 3:3 (2008) 178-181.1815-992310.3923/ajb.2008.176.181info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:21:26Zoai:repositorium.sdum.uminho.pt:1822/7974Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:14:44.241841Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv In vitro antifungal effect of EDTA disodium salt in tested black aspergilli
title In vitro antifungal effect of EDTA disodium salt in tested black aspergilli
spellingShingle In vitro antifungal effect of EDTA disodium salt in tested black aspergilli
Abrunhosa, Luís
AspergrlIus carbonarius
AspergiIlus ibericus
Aspergillus niger
Ochratoxin A
EDTA
Fungicide
Aspergillus carbonarius
Aspergillus ibericus
title_short In vitro antifungal effect of EDTA disodium salt in tested black aspergilli
title_full In vitro antifungal effect of EDTA disodium salt in tested black aspergilli
title_fullStr In vitro antifungal effect of EDTA disodium salt in tested black aspergilli
title_full_unstemmed In vitro antifungal effect of EDTA disodium salt in tested black aspergilli
title_sort In vitro antifungal effect of EDTA disodium salt in tested black aspergilli
author Abrunhosa, Luís
author_facet Abrunhosa, Luís
Venâncio, Armando
author_role author
author2 Venâncio, Armando
author2_role author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Abrunhosa, Luís
Venâncio, Armando
dc.subject.por.fl_str_mv AspergrlIus carbonarius
AspergiIlus ibericus
Aspergillus niger
Ochratoxin A
EDTA
Fungicide
Aspergillus carbonarius
Aspergillus ibericus
topic AspergrlIus carbonarius
AspergiIlus ibericus
Aspergillus niger
Ochratoxin A
EDTA
Fungicide
Aspergillus carbonarius
Aspergillus ibericus
description The antifungal effect of Na2EDTA on an Aspergillus carbonarius, an A. ibericus, an ochratoxigenic A. niger and a non-ochratoxigenic A. niger strain was studied. Also, the effect of Na2EDTA on the production of ochratoxin A by A. carbonarius and the ochratoxigenic A. niger was evaluated. The poisoned food technique was used with CYA medium supplemented with 0, 1 and 10 mmol L-1 of Na2EDTA. The colony diameters were recorded daily and the amount of ochratoxin A produced was quantified every two days. Significant reductions of growth rates were observed in the presence of Na2EDTA being the calculated EC50 of 2.1 mmol L-1 for A. carbonarius, 0.9 mmol L-1 for A. ibericus, 2.0 mmol L-1 for the ochratoxigenic A. niger and 4.1 mmol L-1 for the non-ochratoxigenic A. niger. Furthermore, 10 mmol L-1 Na2EDTA delayed the production of ochratoxin A and reduced the levels in approximately 99% during 8 days. Na2EDTA is frequently used in the food industry and in agriculture agrochemicals and its effects on ochratoxigenic black aspergilli is not well known. This study showed that Na2EDTA can significantly reduce the growth rates of tested fungi and its ochratoxin A production.
publishDate 2008
dc.date.none.fl_str_mv 2008
2008-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/7974
url http://hdl.handle.net/1822/7974
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv "Asian Journal of Biochemistry". ISSN 1815-9923. 3:3 (2008) 178-181.
1815-9923
10.3923/ajb.2008.176.181
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Academic Journals Inc.
publisher.none.fl_str_mv Academic Journals Inc.
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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