The hCOMET project: International database comparison of results with the comet assay in human biomonitoring. Baseline frequency of DNA damage and effect of main confounders
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2021 |
| Outros Autores: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/10400.18/7838 |
Resumo: | Review |
| id |
RCAP_daa1a50c790183c01594a6481f9ca73e |
|---|---|
| oai_identifier_str |
oai:repositorio.insa.pt:10400.18/7838 |
| network_acronym_str |
RCAP |
| network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| repository_id_str |
7160 |
| spelling |
The hCOMET project: International database comparison of results with the comet assay in human biomonitoring. Baseline frequency of DNA damage and effect of main confoundersEnvironmental GenotoxicityDNA DamageComet AssayHuman BiomonitoringPooled AnalysisBiomarkersGenotoxicidade AmbientalGenómica Funcional e EstruturalReviewThe alkaline comet assay, or single cell gel electrophoresis, is one of the most popular methods for assessing DNA damage in human population. One of the open issues concerning this assay is the identification of those factors that can explain the large inter-individual and inter-laboratory variation. International collaborative initiatives such as the hCOMET project - a COST Action launched in 2016 - represent a valuable tool to meet this challenge. The aims of hCOMET were to establish reference values for the level of DNA damage in humans, to investigate the effect of host factors, lifestyle and exposure to genotoxic agents, and to compare different sources of assay variability. A database of 19,320 subjects was generated, pooling data from 105 studies run by 44 laboratories in 26 countries between 1999 and 2019. A mixed random effect log-linear model, in parallel with a classic meta-analysis, was applied to take into account the extensive heterogeneity of data, due to descriptor, specimen and protocol variability. As a result of this analysis interquartile intervals of DNA strand breaks (which includes alkali-labile sites) were reported for tail intensity, tail length, and tail moment (comet assay descriptors). A small variation by age was reported in some datasets, suggesting higher DNA damage in oldest age-classes, while no effect could be shown for sex or smoking habit, although the lack of data on heavy smokers has still to be considered. Finally, highly significant differences in DNA damage were found for most exposures investigated in specific studies. In conclusion, these data, which confirm that DNA damage measured by the comet assay is an excellent biomarker of exposure in several conditions, may contribute to improving the quality of study design and to the standardization of results of the comet assay in human populations.Highlights: The alkaline comet assay is a popular methods for assessing DNA damage in humans; A database of 19,320 subjects from 44 laboratories in 26 countries was analysed; A range of measures estimating baseline DNA damage is provided for %T, TL, and TM; None or limited effect was found for sex, age, and major confounding factors; The comet assay efficiently detected specific genotoxic exposures.COST Action hCOMET CA15132, supported by COST (European Cooperation in Science and Technology www.cost.eu)ElsevierRepositório Científico do Instituto Nacional de SaúdeMilić, MirtaCeppi, MarcelloBruzzone, MarcoAzqueta, AmayaBrunborg, GunnarGodschalk, RogerKoppen, GudrunLangie, SabineMøller, PeterTeixeira, João PauloAlija, AvdullaAnderson, DianaAndrade, VanessaAndreoli, CristinaAsllani, FisnikBangkoglu, Ezgi EyluelBarančoková, MagdalenaBasaran, NursenBoutet-Robinet, ElisaBuschini, AnnamariaCavallo, DeliaCosta Pereira, CristianaCosta, CarlaCosta, SolangeDa Silva, JulianaDel Boˊ, CristianDimitrijević Srećković, VesnaDjelić, NinoslavDobrzyńska, MalgorzataDuračková, ZdenkaDvořáková, MonikaGajski, GoranGalati, SerenaGarcía Lima, OmarGiovannelli, LisaGoroshinskaya, Irina A.Grindel, AnnemarieGutzkow, Kristine B.Hernández, AlbaHernández, CarlosHolven, Kirsten B.Ibero-Baraibar, IdoiaOttestad, IngerKadioglu, ElaKažimirová, AlenaKuznetsova, ElenaLadeira, CarinaLaffon, BlancaLamonaca, PalmaLebailly, PierreLouro, HenriquetaMandina Cardoso, TaniaMarcon, FrancescaMarcos, RicardMoretti, MassimoMoretti, SilviaNajafzadeh, MojganNemeth, ZsuzsannaNeri, MonicaNovotna, BozenaOrlow, IrenePaduchova, ZuzanaPastor, SusanaPerdry, HervéSpremo-Potparević, BiljanaRamadhani, DwiRiso, PatriziaRohr, PaulaRojas, EmilioRossner, PavelSafar, AnnaSardas, SemraSilva, Maria JoãoSirota, NikolaySmolkova, BozenaStaruchova, MartaStetina, RudolfStopper, HelgaSurikova, Ekaterina I.Ulven, Stine M.Ursini, Cinzia LuciaValdiglesias, VanessaValverde, MaharaVodicka, PavelVolkovova, KatarinaWagner, Karl-HeinzŽivković, LadaDušinská, MariaCollins, Andrew R.Bonassi, Stefano2022-01-10T15:32:27Z2021-02-062021-02-06T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.18/7838engMutat Res Rev Mutat Res. Jan-Jun 2021;787:108371. doi: 10.1016/j.mrrev.2021.108371. Epub 2021 Feb 6. Review1383-574210.1016/j.mrrev.2021.108371info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-20T15:42:11Zoai:repositorio.insa.pt:10400.18/7838Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:42:24.394779Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
The hCOMET project: International database comparison of results with the comet assay in human biomonitoring. Baseline frequency of DNA damage and effect of main confounders |
| title |
The hCOMET project: International database comparison of results with the comet assay in human biomonitoring. Baseline frequency of DNA damage and effect of main confounders |
| spellingShingle |
The hCOMET project: International database comparison of results with the comet assay in human biomonitoring. Baseline frequency of DNA damage and effect of main confounders Milić, Mirta Environmental Genotoxicity DNA Damage Comet Assay Human Biomonitoring Pooled Analysis Biomarkers Genotoxicidade Ambiental Genómica Funcional e Estrutural |
| title_short |
The hCOMET project: International database comparison of results with the comet assay in human biomonitoring. Baseline frequency of DNA damage and effect of main confounders |
| title_full |
The hCOMET project: International database comparison of results with the comet assay in human biomonitoring. Baseline frequency of DNA damage and effect of main confounders |
| title_fullStr |
The hCOMET project: International database comparison of results with the comet assay in human biomonitoring. Baseline frequency of DNA damage and effect of main confounders |
| title_full_unstemmed |
The hCOMET project: International database comparison of results with the comet assay in human biomonitoring. Baseline frequency of DNA damage and effect of main confounders |
| title_sort |
The hCOMET project: International database comparison of results with the comet assay in human biomonitoring. Baseline frequency of DNA damage and effect of main confounders |
| author |
Milić, Mirta |
| author_facet |
Milić, Mirta Ceppi, Marcello Bruzzone, Marco Azqueta, Amaya Brunborg, Gunnar Godschalk, Roger Koppen, Gudrun Langie, Sabine Møller, Peter Teixeira, João Paulo Alija, Avdulla Anderson, Diana Andrade, Vanessa Andreoli, Cristina Asllani, Fisnik Bangkoglu, Ezgi Eyluel Barančoková, Magdalena Basaran, Nursen Boutet-Robinet, Elisa Buschini, Annamaria Cavallo, Delia Costa Pereira, Cristiana Costa, Carla Costa, Solange Da Silva, Juliana Del Boˊ, Cristian Dimitrijević Srećković, Vesna Djelić, Ninoslav Dobrzyńska, Malgorzata Duračková, Zdenka Dvořáková, Monika Gajski, Goran Galati, Serena García Lima, Omar Giovannelli, Lisa Goroshinskaya, Irina A. Grindel, Annemarie Gutzkow, Kristine B. Hernández, Alba Hernández, Carlos Holven, Kirsten B. Ibero-Baraibar, Idoia Ottestad, Inger Kadioglu, Ela Kažimirová, Alena Kuznetsova, Elena Ladeira, Carina Laffon, Blanca Lamonaca, Palma Lebailly, Pierre Louro, Henriqueta Mandina Cardoso, Tania Marcon, Francesca Marcos, Ricard Moretti, Massimo Moretti, Silvia Najafzadeh, Mojgan Nemeth, Zsuzsanna Neri, Monica Novotna, Bozena Orlow, Irene Paduchova, Zuzana Pastor, Susana Perdry, Hervé Spremo-Potparević, Biljana Ramadhani, Dwi Riso, Patrizia Rohr, Paula Rojas, Emilio Rossner, Pavel Safar, Anna Sardas, Semra Silva, Maria João Sirota, Nikolay Smolkova, Bozena Staruchova, Marta Stetina, Rudolf Stopper, Helga Surikova, Ekaterina I. Ulven, Stine M. Ursini, Cinzia Lucia Valdiglesias, Vanessa Valverde, Mahara Vodicka, Pavel Volkovova, Katarina Wagner, Karl-Heinz Živković, Lada Dušinská, Maria Collins, Andrew R. Bonassi, Stefano |
| author_role |
author |
| author2 |
Ceppi, Marcello Bruzzone, Marco Azqueta, Amaya Brunborg, Gunnar Godschalk, Roger Koppen, Gudrun Langie, Sabine Møller, Peter Teixeira, João Paulo Alija, Avdulla Anderson, Diana Andrade, Vanessa Andreoli, Cristina Asllani, Fisnik Bangkoglu, Ezgi Eyluel Barančoková, Magdalena Basaran, Nursen Boutet-Robinet, Elisa Buschini, Annamaria Cavallo, Delia Costa Pereira, Cristiana Costa, Carla Costa, Solange Da Silva, Juliana Del Boˊ, Cristian Dimitrijević Srećković, Vesna Djelić, Ninoslav Dobrzyńska, Malgorzata Duračková, Zdenka Dvořáková, Monika Gajski, Goran Galati, Serena García Lima, Omar Giovannelli, Lisa Goroshinskaya, Irina A. Grindel, Annemarie Gutzkow, Kristine B. Hernández, Alba Hernández, Carlos Holven, Kirsten B. Ibero-Baraibar, Idoia Ottestad, Inger Kadioglu, Ela Kažimirová, Alena Kuznetsova, Elena Ladeira, Carina Laffon, Blanca Lamonaca, Palma Lebailly, Pierre Louro, Henriqueta Mandina Cardoso, Tania Marcon, Francesca Marcos, Ricard Moretti, Massimo Moretti, Silvia Najafzadeh, Mojgan Nemeth, Zsuzsanna Neri, Monica Novotna, Bozena Orlow, Irene Paduchova, Zuzana Pastor, Susana Perdry, Hervé Spremo-Potparević, Biljana Ramadhani, Dwi Riso, Patrizia Rohr, Paula Rojas, Emilio Rossner, Pavel Safar, Anna Sardas, Semra Silva, Maria João Sirota, Nikolay Smolkova, Bozena Staruchova, Marta Stetina, Rudolf Stopper, Helga Surikova, Ekaterina I. Ulven, Stine M. Ursini, Cinzia Lucia Valdiglesias, Vanessa Valverde, Mahara Vodicka, Pavel Volkovova, Katarina Wagner, Karl-Heinz Živković, Lada Dušinská, Maria Collins, Andrew R. Bonassi, Stefano |
| author2_role |
author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Repositório Científico do Instituto Nacional de Saúde |
| dc.contributor.author.fl_str_mv |
Milić, Mirta Ceppi, Marcello Bruzzone, Marco Azqueta, Amaya Brunborg, Gunnar Godschalk, Roger Koppen, Gudrun Langie, Sabine Møller, Peter Teixeira, João Paulo Alija, Avdulla Anderson, Diana Andrade, Vanessa Andreoli, Cristina Asllani, Fisnik Bangkoglu, Ezgi Eyluel Barančoková, Magdalena Basaran, Nursen Boutet-Robinet, Elisa Buschini, Annamaria Cavallo, Delia Costa Pereira, Cristiana Costa, Carla Costa, Solange Da Silva, Juliana Del Boˊ, Cristian Dimitrijević Srećković, Vesna Djelić, Ninoslav Dobrzyńska, Malgorzata Duračková, Zdenka Dvořáková, Monika Gajski, Goran Galati, Serena García Lima, Omar Giovannelli, Lisa Goroshinskaya, Irina A. Grindel, Annemarie Gutzkow, Kristine B. Hernández, Alba Hernández, Carlos Holven, Kirsten B. Ibero-Baraibar, Idoia Ottestad, Inger Kadioglu, Ela Kažimirová, Alena Kuznetsova, Elena Ladeira, Carina Laffon, Blanca Lamonaca, Palma Lebailly, Pierre Louro, Henriqueta Mandina Cardoso, Tania Marcon, Francesca Marcos, Ricard Moretti, Massimo Moretti, Silvia Najafzadeh, Mojgan Nemeth, Zsuzsanna Neri, Monica Novotna, Bozena Orlow, Irene Paduchova, Zuzana Pastor, Susana Perdry, Hervé Spremo-Potparević, Biljana Ramadhani, Dwi Riso, Patrizia Rohr, Paula Rojas, Emilio Rossner, Pavel Safar, Anna Sardas, Semra Silva, Maria João Sirota, Nikolay Smolkova, Bozena Staruchova, Marta Stetina, Rudolf Stopper, Helga Surikova, Ekaterina I. Ulven, Stine M. Ursini, Cinzia Lucia Valdiglesias, Vanessa Valverde, Mahara Vodicka, Pavel Volkovova, Katarina Wagner, Karl-Heinz Živković, Lada Dušinská, Maria Collins, Andrew R. Bonassi, Stefano |
| dc.subject.por.fl_str_mv |
Environmental Genotoxicity DNA Damage Comet Assay Human Biomonitoring Pooled Analysis Biomarkers Genotoxicidade Ambiental Genómica Funcional e Estrutural |
| topic |
Environmental Genotoxicity DNA Damage Comet Assay Human Biomonitoring Pooled Analysis Biomarkers Genotoxicidade Ambiental Genómica Funcional e Estrutural |
| description |
Review |
| publishDate |
2021 |
| dc.date.none.fl_str_mv |
2021-02-06 2021-02-06T00:00:00Z 2022-01-10T15:32:27Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.18/7838 |
| url |
http://hdl.handle.net/10400.18/7838 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
Mutat Res Rev Mutat Res. Jan-Jun 2021;787:108371. doi: 10.1016/j.mrrev.2021.108371. Epub 2021 Feb 6. Review 1383-5742 10.1016/j.mrrev.2021.108371 |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.publisher.none.fl_str_mv |
Elsevier |
| publisher.none.fl_str_mv |
Elsevier |
| dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
| instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
| instacron_str |
RCAAP |
| institution |
RCAAP |
| reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
| repository.mail.fl_str_mv |
|
| _version_ |
1799132169478602752 |