VISCERAL INFECTION OF PUMPKINSEED SUNFISH (Lepomis gibbosus) WITH Posthodiplostomum minimum centrarchi – ULTRASTRUCTURAL STUDY

Detalhes bibliográficos
Autor(a) principal: Ramos, Paula
Data de Publicação: 2012
Outros Autores: Pereira da Fonseca, Isabel, Branco, Sandra, Alexandre-Pires, Graça
Tipo de documento: Artigo de conferência
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10174/5348
Resumo: VISCERAL INFECTION OF PUMPKINSEED SUNFISH (Lepomis gibbosus) WITH Posthodiplostomum minimum centrarchi – ULTRASTRUCTURAL STUDY P. Ramos1., I. Pereira da Fonseca2, S. Branco3, G. Alexandre-Pires 2 1 Unidade de Aquacultura, INRB, IP/IPIMAR, Av. de Brasília, Lisboa, Portugal 2 Centro de Investigação Interdisciplinar em Sanidade Animal, FMV, UTL, Av. Universidade Técnica, 1300-477 Lisboa, Portugal 3 Instituto de Ciências Agrárias e Ambientais Mediterrânicas (ICAAM). Universidade de Évora Introduction: Heart, kidneys, liver and spleen of freshwater fish Lepomis gibbosus were found infected by Posthodiplostomum minimum centrarchi metacercariae. Visceral organs contain macroscopically visible white cysts. Ultrastructural studies of the cysts, parasites and infected tissues were performed in order to access tissues damage and a better characterization of the parasite. Material and Methods: Visceral samples obtained from twenty specimens of fresh pumpkinseed sunfish captured in a dam located at the centre of Portugal were submitted to Scanning Electronic Microscopy (SEM) techniques and histological routine methods. Results: Histological examination of the samples revealed numerous cysts; empty cysts and cysts with parasites present in the parenchyma of spleen, liver and kidneys; and pericardial region of the ventricle. They were surrounded by a cellular inflammatory response by the accumulation of macrophages, eosinophils and polymorphonuclear neutrophils. Melanomacrophage centres (MMC) were diffusely scattered through spleen and liver. The cyst shows two well discernable walls, the inner wall of the parasite origin and the outer fibroblastic capsule of host origin. A huge amount of calcareous concretions filled and surrounding the metarcercariae. Conclusion: Although the host reaction to these parasites is a minimal response, the proliferation of MMC was associated with the infection. The metacercariae frequently twisted upon themselves in histological sections, so that discerning the relationship among their internal organs is often difficult. In this work, SEM technique was used to show specific three-dimensional morphological details of the key features used to identify trematodes parasites by optical microscopy, like the oral and ventral suckers attached to the host, their position and relative size, being complementary to the understanding of the histopathological effects inflicted to host tissues.
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spelling VISCERAL INFECTION OF PUMPKINSEED SUNFISH (Lepomis gibbosus) WITH Posthodiplostomum minimum centrarchi – ULTRASTRUCTURAL STUDYPUMPKINSEED SUNFISHPosthodiplostomum minimum centrarchiVISCERAL INFECTION OF PUMPKINSEED SUNFISH (Lepomis gibbosus) WITH Posthodiplostomum minimum centrarchi – ULTRASTRUCTURAL STUDY P. Ramos1., I. Pereira da Fonseca2, S. Branco3, G. Alexandre-Pires 2 1 Unidade de Aquacultura, INRB, IP/IPIMAR, Av. de Brasília, Lisboa, Portugal 2 Centro de Investigação Interdisciplinar em Sanidade Animal, FMV, UTL, Av. Universidade Técnica, 1300-477 Lisboa, Portugal 3 Instituto de Ciências Agrárias e Ambientais Mediterrânicas (ICAAM). Universidade de Évora Introduction: Heart, kidneys, liver and spleen of freshwater fish Lepomis gibbosus were found infected by Posthodiplostomum minimum centrarchi metacercariae. Visceral organs contain macroscopically visible white cysts. Ultrastructural studies of the cysts, parasites and infected tissues were performed in order to access tissues damage and a better characterization of the parasite. Material and Methods: Visceral samples obtained from twenty specimens of fresh pumpkinseed sunfish captured in a dam located at the centre of Portugal were submitted to Scanning Electronic Microscopy (SEM) techniques and histological routine methods. Results: Histological examination of the samples revealed numerous cysts; empty cysts and cysts with parasites present in the parenchyma of spleen, liver and kidneys; and pericardial region of the ventricle. They were surrounded by a cellular inflammatory response by the accumulation of macrophages, eosinophils and polymorphonuclear neutrophils. Melanomacrophage centres (MMC) were diffusely scattered through spleen and liver. The cyst shows two well discernable walls, the inner wall of the parasite origin and the outer fibroblastic capsule of host origin. A huge amount of calcareous concretions filled and surrounding the metarcercariae. Conclusion: Although the host reaction to these parasites is a minimal response, the proliferation of MMC was associated with the infection. The metacercariae frequently twisted upon themselves in histological sections, so that discerning the relationship among their internal organs is often difficult. In this work, SEM technique was used to show specific three-dimensional morphological details of the key features used to identify trematodes parasites by optical microscopy, like the oral and ventral suckers attached to the host, their position and relative size, being complementary to the understanding of the histopathological effects inflicted to host tissues.Bulgarian Journal of Veterinary Medicine2012-10-15T11:13:42Z2012-10-152012-07-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjecthttp://hdl.handle.net/10174/5348http://hdl.handle.net/10174/5348engBJVM,15,Suppl.1,2012,pag.87naonaosimpramos@ipma.ptndsmbb@uevora.ptnd384Ramos, PaulaPereira da Fonseca, IsabelBranco, SandraAlexandre-Pires, Graçainfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-03T18:43:54Zoai:dspace.uevora.pt:10174/5348Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:00:18.777697Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv VISCERAL INFECTION OF PUMPKINSEED SUNFISH (Lepomis gibbosus) WITH Posthodiplostomum minimum centrarchi – ULTRASTRUCTURAL STUDY
title VISCERAL INFECTION OF PUMPKINSEED SUNFISH (Lepomis gibbosus) WITH Posthodiplostomum minimum centrarchi – ULTRASTRUCTURAL STUDY
spellingShingle VISCERAL INFECTION OF PUMPKINSEED SUNFISH (Lepomis gibbosus) WITH Posthodiplostomum minimum centrarchi – ULTRASTRUCTURAL STUDY
Ramos, Paula
PUMPKINSEED SUNFISH
Posthodiplostomum minimum centrarchi
title_short VISCERAL INFECTION OF PUMPKINSEED SUNFISH (Lepomis gibbosus) WITH Posthodiplostomum minimum centrarchi – ULTRASTRUCTURAL STUDY
title_full VISCERAL INFECTION OF PUMPKINSEED SUNFISH (Lepomis gibbosus) WITH Posthodiplostomum minimum centrarchi – ULTRASTRUCTURAL STUDY
title_fullStr VISCERAL INFECTION OF PUMPKINSEED SUNFISH (Lepomis gibbosus) WITH Posthodiplostomum minimum centrarchi – ULTRASTRUCTURAL STUDY
title_full_unstemmed VISCERAL INFECTION OF PUMPKINSEED SUNFISH (Lepomis gibbosus) WITH Posthodiplostomum minimum centrarchi – ULTRASTRUCTURAL STUDY
title_sort VISCERAL INFECTION OF PUMPKINSEED SUNFISH (Lepomis gibbosus) WITH Posthodiplostomum minimum centrarchi – ULTRASTRUCTURAL STUDY
author Ramos, Paula
author_facet Ramos, Paula
Pereira da Fonseca, Isabel
Branco, Sandra
Alexandre-Pires, Graça
author_role author
author2 Pereira da Fonseca, Isabel
Branco, Sandra
Alexandre-Pires, Graça
author2_role author
author
author
dc.contributor.author.fl_str_mv Ramos, Paula
Pereira da Fonseca, Isabel
Branco, Sandra
Alexandre-Pires, Graça
dc.subject.por.fl_str_mv PUMPKINSEED SUNFISH
Posthodiplostomum minimum centrarchi
topic PUMPKINSEED SUNFISH
Posthodiplostomum minimum centrarchi
description VISCERAL INFECTION OF PUMPKINSEED SUNFISH (Lepomis gibbosus) WITH Posthodiplostomum minimum centrarchi – ULTRASTRUCTURAL STUDY P. Ramos1., I. Pereira da Fonseca2, S. Branco3, G. Alexandre-Pires 2 1 Unidade de Aquacultura, INRB, IP/IPIMAR, Av. de Brasília, Lisboa, Portugal 2 Centro de Investigação Interdisciplinar em Sanidade Animal, FMV, UTL, Av. Universidade Técnica, 1300-477 Lisboa, Portugal 3 Instituto de Ciências Agrárias e Ambientais Mediterrânicas (ICAAM). Universidade de Évora Introduction: Heart, kidneys, liver and spleen of freshwater fish Lepomis gibbosus were found infected by Posthodiplostomum minimum centrarchi metacercariae. Visceral organs contain macroscopically visible white cysts. Ultrastructural studies of the cysts, parasites and infected tissues were performed in order to access tissues damage and a better characterization of the parasite. Material and Methods: Visceral samples obtained from twenty specimens of fresh pumpkinseed sunfish captured in a dam located at the centre of Portugal were submitted to Scanning Electronic Microscopy (SEM) techniques and histological routine methods. Results: Histological examination of the samples revealed numerous cysts; empty cysts and cysts with parasites present in the parenchyma of spleen, liver and kidneys; and pericardial region of the ventricle. They were surrounded by a cellular inflammatory response by the accumulation of macrophages, eosinophils and polymorphonuclear neutrophils. Melanomacrophage centres (MMC) were diffusely scattered through spleen and liver. The cyst shows two well discernable walls, the inner wall of the parasite origin and the outer fibroblastic capsule of host origin. A huge amount of calcareous concretions filled and surrounding the metarcercariae. Conclusion: Although the host reaction to these parasites is a minimal response, the proliferation of MMC was associated with the infection. The metacercariae frequently twisted upon themselves in histological sections, so that discerning the relationship among their internal organs is often difficult. In this work, SEM technique was used to show specific three-dimensional morphological details of the key features used to identify trematodes parasites by optical microscopy, like the oral and ventral suckers attached to the host, their position and relative size, being complementary to the understanding of the histopathological effects inflicted to host tissues.
publishDate 2012
dc.date.none.fl_str_mv 2012-10-15T11:13:42Z
2012-10-15
2012-07-01T00:00:00Z
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dc.identifier.uri.fl_str_mv http://hdl.handle.net/10174/5348
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dc.relation.none.fl_str_mv BJVM,15,Suppl.1,2012,pag.87
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pramos@ipma.pt
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dc.publisher.none.fl_str_mv Bulgarian Journal of Veterinary Medicine
publisher.none.fl_str_mv Bulgarian Journal of Veterinary Medicine
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