Nickel nanoparticles induces cytotoxicity, cell morphology and oxidative stress in bluegill sunfish (BF-2) cells
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10773/37428 |
Resumo: | The rationale of the current study was to assess the suitability of BF-2 cell line as a model to assess nanotoxicity in the caudal fin cells of bluegill sunfish in vitro. The current study investigates the potential toxicity, morphological changes and oxidative stress of nickel nanoparticles (Ni NPs) in bluegill sunfish cells (BF-2) using mitochondrial, neutral red uptake and lactate dehydrogenase assays. Results indicated a concentration dependent cytotoxic effect after 24 h in mitochondrial, lysosomal and lactate dehydrogenase activities. BF-2 cells morphology was altered when exposed to 30 μg ml−1 concentrations of Ni NPs for 24 h. Dose dependent increase of oxidative stress was evidenced in BF-2 cells when exposed to Ni NPs, showed significant escalation in peroxidation of lipids (LPO), protein carbonyl (PC), glutathione sulfo-transferase (GST) and glutathione peroxidase (GPX) as compared to their experimental controls. However, the catalase (CAT) and total glutathione content (TGSH) was found to decrease dose dependently in BF-2 cells exposed to Ni NPs. The current study demonstrated that BF-2 cells may serve as a sensitive indicator for aquatic contaminant evaluations in toxicological research. |
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Nickel nanoparticles induces cytotoxicity, cell morphology and oxidative stress in bluegill sunfish (BF-2) cellsBlue gill sunfishNickel nanoparticlesCytotoxicityMorphologyOxidative stressThe rationale of the current study was to assess the suitability of BF-2 cell line as a model to assess nanotoxicity in the caudal fin cells of bluegill sunfish in vitro. The current study investigates the potential toxicity, morphological changes and oxidative stress of nickel nanoparticles (Ni NPs) in bluegill sunfish cells (BF-2) using mitochondrial, neutral red uptake and lactate dehydrogenase assays. Results indicated a concentration dependent cytotoxic effect after 24 h in mitochondrial, lysosomal and lactate dehydrogenase activities. BF-2 cells morphology was altered when exposed to 30 μg ml−1 concentrations of Ni NPs for 24 h. Dose dependent increase of oxidative stress was evidenced in BF-2 cells when exposed to Ni NPs, showed significant escalation in peroxidation of lipids (LPO), protein carbonyl (PC), glutathione sulfo-transferase (GST) and glutathione peroxidase (GPX) as compared to their experimental controls. However, the catalase (CAT) and total glutathione content (TGSH) was found to decrease dose dependently in BF-2 cells exposed to Ni NPs. The current study demonstrated that BF-2 cells may serve as a sensitive indicator for aquatic contaminant evaluations in toxicological research.Elsevier2023-04-27T15:38:02Z2019-07-31T00:00:00Z2019-07-31info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10773/37428eng0169-433210.1016/j.apsusc.2019.03.255Poornavaishnavi, ChekuriGowthami, ReddivarySrikanth, KoigooraBramhachari, P.V.Venkatramaiah, N.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-05-06T04:45:23Zoai:ria.ua.pt:10773/37428Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-05-06T04:45:23Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Nickel nanoparticles induces cytotoxicity, cell morphology and oxidative stress in bluegill sunfish (BF-2) cells |
title |
Nickel nanoparticles induces cytotoxicity, cell morphology and oxidative stress in bluegill sunfish (BF-2) cells |
spellingShingle |
Nickel nanoparticles induces cytotoxicity, cell morphology and oxidative stress in bluegill sunfish (BF-2) cells Poornavaishnavi, Chekuri Blue gill sunfish Nickel nanoparticles Cytotoxicity Morphology Oxidative stress |
title_short |
Nickel nanoparticles induces cytotoxicity, cell morphology and oxidative stress in bluegill sunfish (BF-2) cells |
title_full |
Nickel nanoparticles induces cytotoxicity, cell morphology and oxidative stress in bluegill sunfish (BF-2) cells |
title_fullStr |
Nickel nanoparticles induces cytotoxicity, cell morphology and oxidative stress in bluegill sunfish (BF-2) cells |
title_full_unstemmed |
Nickel nanoparticles induces cytotoxicity, cell morphology and oxidative stress in bluegill sunfish (BF-2) cells |
title_sort |
Nickel nanoparticles induces cytotoxicity, cell morphology and oxidative stress in bluegill sunfish (BF-2) cells |
author |
Poornavaishnavi, Chekuri |
author_facet |
Poornavaishnavi, Chekuri Gowthami, Reddivary Srikanth, Koigoora Bramhachari, P.V. Venkatramaiah, N. |
author_role |
author |
author2 |
Gowthami, Reddivary Srikanth, Koigoora Bramhachari, P.V. Venkatramaiah, N. |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Poornavaishnavi, Chekuri Gowthami, Reddivary Srikanth, Koigoora Bramhachari, P.V. Venkatramaiah, N. |
dc.subject.por.fl_str_mv |
Blue gill sunfish Nickel nanoparticles Cytotoxicity Morphology Oxidative stress |
topic |
Blue gill sunfish Nickel nanoparticles Cytotoxicity Morphology Oxidative stress |
description |
The rationale of the current study was to assess the suitability of BF-2 cell line as a model to assess nanotoxicity in the caudal fin cells of bluegill sunfish in vitro. The current study investigates the potential toxicity, morphological changes and oxidative stress of nickel nanoparticles (Ni NPs) in bluegill sunfish cells (BF-2) using mitochondrial, neutral red uptake and lactate dehydrogenase assays. Results indicated a concentration dependent cytotoxic effect after 24 h in mitochondrial, lysosomal and lactate dehydrogenase activities. BF-2 cells morphology was altered when exposed to 30 μg ml−1 concentrations of Ni NPs for 24 h. Dose dependent increase of oxidative stress was evidenced in BF-2 cells when exposed to Ni NPs, showed significant escalation in peroxidation of lipids (LPO), protein carbonyl (PC), glutathione sulfo-transferase (GST) and glutathione peroxidase (GPX) as compared to their experimental controls. However, the catalase (CAT) and total glutathione content (TGSH) was found to decrease dose dependently in BF-2 cells exposed to Ni NPs. The current study demonstrated that BF-2 cells may serve as a sensitive indicator for aquatic contaminant evaluations in toxicological research. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-07-31T00:00:00Z 2019-07-31 2023-04-27T15:38:02Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10773/37428 |
url |
http://hdl.handle.net/10773/37428 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
0169-4332 10.1016/j.apsusc.2019.03.255 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
mluisa.alvim@gmail.com |
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1817543854563262464 |