Nickel nanoparticles induces cytotoxicity, cell morphology and oxidative stress in bluegill sunfish (BF-2) cells

Detalhes bibliográficos
Autor(a) principal: Poornavaishnavi, Chekuri
Data de Publicação: 2019
Outros Autores: Gowthami, Reddivary, Srikanth, Koigoora, Bramhachari, P.V., Venkatramaiah, N.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10773/37428
Resumo: The rationale of the current study was to assess the suitability of BF-2 cell line as a model to assess nanotoxicity in the caudal fin cells of bluegill sunfish in vitro. The current study investigates the potential toxicity, morphological changes and oxidative stress of nickel nanoparticles (Ni NPs) in bluegill sunfish cells (BF-2) using mitochondrial, neutral red uptake and lactate dehydrogenase assays. Results indicated a concentration dependent cytotoxic effect after 24 h in mitochondrial, lysosomal and lactate dehydrogenase activities. BF-2 cells morphology was altered when exposed to 30 μg ml−1 concentrations of Ni NPs for 24 h. Dose dependent increase of oxidative stress was evidenced in BF-2 cells when exposed to Ni NPs, showed significant escalation in peroxidation of lipids (LPO), protein carbonyl (PC), glutathione sulfo-transferase (GST) and glutathione peroxidase (GPX) as compared to their experimental controls. However, the catalase (CAT) and total glutathione content (TGSH) was found to decrease dose dependently in BF-2 cells exposed to Ni NPs. The current study demonstrated that BF-2 cells may serve as a sensitive indicator for aquatic contaminant evaluations in toxicological research.
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spelling Nickel nanoparticles induces cytotoxicity, cell morphology and oxidative stress in bluegill sunfish (BF-2) cellsBlue gill sunfishNickel nanoparticlesCytotoxicityMorphologyOxidative stressThe rationale of the current study was to assess the suitability of BF-2 cell line as a model to assess nanotoxicity in the caudal fin cells of bluegill sunfish in vitro. The current study investigates the potential toxicity, morphological changes and oxidative stress of nickel nanoparticles (Ni NPs) in bluegill sunfish cells (BF-2) using mitochondrial, neutral red uptake and lactate dehydrogenase assays. Results indicated a concentration dependent cytotoxic effect after 24 h in mitochondrial, lysosomal and lactate dehydrogenase activities. BF-2 cells morphology was altered when exposed to 30 μg ml−1 concentrations of Ni NPs for 24 h. Dose dependent increase of oxidative stress was evidenced in BF-2 cells when exposed to Ni NPs, showed significant escalation in peroxidation of lipids (LPO), protein carbonyl (PC), glutathione sulfo-transferase (GST) and glutathione peroxidase (GPX) as compared to their experimental controls. However, the catalase (CAT) and total glutathione content (TGSH) was found to decrease dose dependently in BF-2 cells exposed to Ni NPs. The current study demonstrated that BF-2 cells may serve as a sensitive indicator for aquatic contaminant evaluations in toxicological research.Elsevier2023-04-27T15:38:02Z2019-07-31T00:00:00Z2019-07-31info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10773/37428eng0169-433210.1016/j.apsusc.2019.03.255Poornavaishnavi, ChekuriGowthami, ReddivarySrikanth, KoigooraBramhachari, P.V.Venkatramaiah, N.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-05-06T04:45:23Zoai:ria.ua.pt:10773/37428Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-05-06T04:45:23Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Nickel nanoparticles induces cytotoxicity, cell morphology and oxidative stress in bluegill sunfish (BF-2) cells
title Nickel nanoparticles induces cytotoxicity, cell morphology and oxidative stress in bluegill sunfish (BF-2) cells
spellingShingle Nickel nanoparticles induces cytotoxicity, cell morphology and oxidative stress in bluegill sunfish (BF-2) cells
Poornavaishnavi, Chekuri
Blue gill sunfish
Nickel nanoparticles
Cytotoxicity
Morphology
Oxidative stress
title_short Nickel nanoparticles induces cytotoxicity, cell morphology and oxidative stress in bluegill sunfish (BF-2) cells
title_full Nickel nanoparticles induces cytotoxicity, cell morphology and oxidative stress in bluegill sunfish (BF-2) cells
title_fullStr Nickel nanoparticles induces cytotoxicity, cell morphology and oxidative stress in bluegill sunfish (BF-2) cells
title_full_unstemmed Nickel nanoparticles induces cytotoxicity, cell morphology and oxidative stress in bluegill sunfish (BF-2) cells
title_sort Nickel nanoparticles induces cytotoxicity, cell morphology and oxidative stress in bluegill sunfish (BF-2) cells
author Poornavaishnavi, Chekuri
author_facet Poornavaishnavi, Chekuri
Gowthami, Reddivary
Srikanth, Koigoora
Bramhachari, P.V.
Venkatramaiah, N.
author_role author
author2 Gowthami, Reddivary
Srikanth, Koigoora
Bramhachari, P.V.
Venkatramaiah, N.
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Poornavaishnavi, Chekuri
Gowthami, Reddivary
Srikanth, Koigoora
Bramhachari, P.V.
Venkatramaiah, N.
dc.subject.por.fl_str_mv Blue gill sunfish
Nickel nanoparticles
Cytotoxicity
Morphology
Oxidative stress
topic Blue gill sunfish
Nickel nanoparticles
Cytotoxicity
Morphology
Oxidative stress
description The rationale of the current study was to assess the suitability of BF-2 cell line as a model to assess nanotoxicity in the caudal fin cells of bluegill sunfish in vitro. The current study investigates the potential toxicity, morphological changes and oxidative stress of nickel nanoparticles (Ni NPs) in bluegill sunfish cells (BF-2) using mitochondrial, neutral red uptake and lactate dehydrogenase assays. Results indicated a concentration dependent cytotoxic effect after 24 h in mitochondrial, lysosomal and lactate dehydrogenase activities. BF-2 cells morphology was altered when exposed to 30 μg ml−1 concentrations of Ni NPs for 24 h. Dose dependent increase of oxidative stress was evidenced in BF-2 cells when exposed to Ni NPs, showed significant escalation in peroxidation of lipids (LPO), protein carbonyl (PC), glutathione sulfo-transferase (GST) and glutathione peroxidase (GPX) as compared to their experimental controls. However, the catalase (CAT) and total glutathione content (TGSH) was found to decrease dose dependently in BF-2 cells exposed to Ni NPs. The current study demonstrated that BF-2 cells may serve as a sensitive indicator for aquatic contaminant evaluations in toxicological research.
publishDate 2019
dc.date.none.fl_str_mv 2019-07-31T00:00:00Z
2019-07-31
2023-04-27T15:38:02Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10773/37428
url http://hdl.handle.net/10773/37428
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0169-4332
10.1016/j.apsusc.2019.03.255
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv mluisa.alvim@gmail.com
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